Molecular epidemiology of mycobacteria among herds in Marajó Island, Brazil, reveals strains genetically related and potential zoonotic risk of clinical relevance

• Autor(a) principal: Furlaneto, Ismari Perini
• Data de Publicação: 2019
• Outros Autores: Conceição, Marília Lima da, Conceição, Emilyn Costa, Lopes, Maria Luíza, Rodrigues, Yan Corrêa, Macelino, Beatriz Reis, Gomes, Harrison Magdinier, Suffys, Philip Noel, Guimarães, Ricardo José de Paula Souza e, Silva, Marlei Gomes da, Duarte, Rafael Silva, Francez, Loreno da Costa, Casseb, Alexandre do Rosário, Câmara, Volney de Magalhães, Pereira, Washington Luiz Assunção, Costa, Ana Roberta Fusco da, Lima, Karla Valéria Batista
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3977
• topic: Tuberculose Bovina; Mycobacterium bovis / patogenicidade; Epidemiologia Molecular; Bovinos; Ilha do Marajó (PA); Abate de Animais; Consumo de Alimentos

Mycobacterium bovis is the main causative agent of bovine tuberculosis (bTB) being among the animal-adapted Mycobacterium tuberculosis complex. Herds can also be infected with non-tuberculous mycobacteria (NTM) causing a negative effect on the economy and on animal and human health through zoonotic infections. Molecular tools are required for mycobacteria identification; thus, it is laborious to determine the epidemiological information of mycobacteria among herds. We aimed to describe the mycobacterial pathogens associated with cases of suspected bTB lesions in cattle/buffaloes slaughtered for consumption and to investigate bTB transmission. We evaluated 74 lesion samples from 48 animals (27 bovine/21 buffaloes) from 16 mapped farms. Positives samples from nested-PCR were cultured in Lowenstein-Jensen (LJ), 2% pyruvate (LJ + P), and 2% glycerol (LJ + G) media, followed by Ziehl-Neelsen (ZN) staining technique and partial gene sequencing (hsp65, rpoB, and 16S-rRNA). Spoligotyping and 24-MIRU-VNTR were performed. The LJ + P increased the chance of obtaining bacilli. The respiratory tract and the oral cavity were the most important infection route. In addition, the calcified part of the lesions suggested chronic bTB. Spoligotypes of M. bovis (SIT986/SB0885) differed from others found in South America, and the MIRU-VNTR 24 loci suggested that bTB was associated to highly related strains. The NTM species found are of clinical importance in humans.