Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections
Autor(a) principal: | |
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Data de Publicação: | 1983 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3198 |
Resumo: | Summary: Accurate diagnosis offatal Yellow Fever (YF) is important for surveillance and prevention of epidemics in regions where the virus is endemic. Immunoperoxidase (IP) staining for viral antigens offers a means to both document infection and investigate pathogenic mechanisms. We developed an indirect IP staining assay for YF, which was used to detect YF antigen in histologic sections of human liver from patients with fatal natural infections, and suckling mouse brains experimentally inoculated with an unattenuated neurotropic strain of YF virus. In human liver, specific IP staining was present in the cytoplasm of hepatocytes, but in mouse brain, specific IP staining was localized within nuclei of neurons. The distribution of IP staining corresponded to the location of degenerative morphologic changes within cells; however, IP staining was more widespread than expected on the basis of histopathologic lesions. Furthermore, the degree of inflammation was disproportionately mild compared to the extent of infection, i.e. distribution af YF antigen, both in human liver and suckling mouse brain. The results suggest hat the pathogenesis of YF in humans and suckling mice is overwhelming viral infection with minimal associated host immunological and / or inflammatory response in tissues |
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De La Monte, S. MLinhares, Alexandre da CostaRosa, Amélia Paes de Andrade Travassos daPinheiro, Francisco de Paula2018-07-10T16:43:57Z2018-07-10T16:43:57Z1983De LA MONTE, S. M. et al. Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections. Tropical and Geographical Medicine, v. 35, n. 3, p. 235-241, Sept. 1983.0041-3232https://patua.iec.gov.br/handle/iec/3198Summary: Accurate diagnosis offatal Yellow Fever (YF) is important for surveillance and prevention of epidemics in regions where the virus is endemic. Immunoperoxidase (IP) staining for viral antigens offers a means to both document infection and investigate pathogenic mechanisms. We developed an indirect IP staining assay for YF, which was used to detect YF antigen in histologic sections of human liver from patients with fatal natural infections, and suckling mouse brains experimentally inoculated with an unattenuated neurotropic strain of YF virus. In human liver, specific IP staining was present in the cytoplasm of hepatocytes, but in mouse brain, specific IP staining was localized within nuclei of neurons. The distribution of IP staining corresponded to the location of degenerative morphologic changes within cells; however, IP staining was more widespread than expected on the basis of histopathologic lesions. Furthermore, the degree of inflammation was disproportionately mild compared to the extent of infection, i.e. distribution af YF antigen, both in human liver and suckling mouse brain. The results suggest hat the pathogenesis of YF in humans and suckling mice is overwhelming viral infection with minimal associated host immunological and / or inflammatory response in tissuesThe Johns Hopkins Hospital. Department of Pathology. Baltimore, Maryland, USA.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.engImmunoperoxidase detection of Yellow Fever virus after natural and experimental infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCérebro / imunologiaAntígenos / análiseFígado / imunologiaFebre Amarela / diagnósticoVírus da Febre Amarela / imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdfImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdfapplication/pdf1136868https://patua.iec.gov.br/bitstreams/ca66d8fc-d52b-49ad-9a0a-44b76170b01e/download4cea4028ffce69f8869dec5fc4322ffaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/1926e18e-0f47-4603-a121-ab3ddcba02a4/download52f1732ea66fbd1123abe39f5373b797MD52TEXTImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdf.txtImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdf.txtExtracted texttext/plain20754https://patua.iec.gov.br/bitstreams/6e7e07b4-75e0-4b6d-aa95-ee6477c79599/download4a3c229a44bd413e7461dded066e1e4eMD55THUMBNAILImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdf.jpgImmunoperoxidase detection of Yellow Fever virus after natural and experimental infections.pdf.jpgGenerated Thumbnailimage/jpeg5994https://patua.iec.gov.br/bitstreams/d6c45030-e8f0-4847-b380-224c068e370a/download22f6a93746deea2e16c2529a85753fc8MD56iec/31982022-10-20 23:24:26.369oai:patua.iec.gov.br:iec/3198https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:24:26Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
title |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
spellingShingle |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections De La Monte, S. M Cérebro / imunologia Antígenos / análise Fígado / imunologia Febre Amarela / diagnóstico Vírus da Febre Amarela / imunologia |
title_short |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
title_full |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
title_fullStr |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
title_full_unstemmed |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
title_sort |
Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections |
author |
De La Monte, S. M |
author_facet |
De La Monte, S. M Linhares, Alexandre da Costa Rosa, Amélia Paes de Andrade Travassos da Pinheiro, Francisco de Paula |
author_role |
author |
author2 |
Linhares, Alexandre da Costa Rosa, Amélia Paes de Andrade Travassos da Pinheiro, Francisco de Paula |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
De La Monte, S. M Linhares, Alexandre da Costa Rosa, Amélia Paes de Andrade Travassos da Pinheiro, Francisco de Paula |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Cérebro / imunologia Antígenos / análise Fígado / imunologia Febre Amarela / diagnóstico Vírus da Febre Amarela / imunologia |
topic |
Cérebro / imunologia Antígenos / análise Fígado / imunologia Febre Amarela / diagnóstico Vírus da Febre Amarela / imunologia |
description |
Summary: Accurate diagnosis offatal Yellow Fever (YF) is important for surveillance and prevention of epidemics in regions where the virus is endemic. Immunoperoxidase (IP) staining for viral antigens offers a means to both document infection and investigate pathogenic mechanisms. We developed an indirect IP staining assay for YF, which was used to detect YF antigen in histologic sections of human liver from patients with fatal natural infections, and suckling mouse brains experimentally inoculated with an unattenuated neurotropic strain of YF virus. In human liver, specific IP staining was present in the cytoplasm of hepatocytes, but in mouse brain, specific IP staining was localized within nuclei of neurons. The distribution of IP staining corresponded to the location of degenerative morphologic changes within cells; however, IP staining was more widespread than expected on the basis of histopathologic lesions. Furthermore, the degree of inflammation was disproportionately mild compared to the extent of infection, i.e. distribution af YF antigen, both in human liver and suckling mouse brain. The results suggest hat the pathogenesis of YF in humans and suckling mice is overwhelming viral infection with minimal associated host immunological and / or inflammatory response in tissues |
publishDate |
1983 |
dc.date.issued.fl_str_mv |
1983 |
dc.date.accessioned.fl_str_mv |
2018-07-10T16:43:57Z |
dc.date.available.fl_str_mv |
2018-07-10T16:43:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
De LA MONTE, S. M. et al. Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections. Tropical and Geographical Medicine, v. 35, n. 3, p. 235-241, Sept. 1983. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3198 |
dc.identifier.issn.-.fl_str_mv |
0041-3232 |
identifier_str_mv |
De LA MONTE, S. M. et al. Immunoperoxidase detection of Yellow Fever virus after natural and experimental infections. Tropical and Geographical Medicine, v. 35, n. 3, p. 235-241, Sept. 1983. 0041-3232 |
url |
https://patua.iec.gov.br/handle/iec/3198 |
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