Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum
Autor(a) principal: | |
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Data de Publicação: | 1984 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2776 |
Resumo: | Clones of an isolate of Plasmodium falciparum from Mae Sod (Thailand) were prepared by a dilution procedure. Some of the parasite cultures thus obtained have been typed for the following characters: (i) electrophoretic variants of three enzymes; (ii) susceptibility to chloroquine and pyrimethamine; (iii) antigen diversities recognized by ten strain-specific monoclonal antibodies; (iv) presence or absence of knobs on infected erythrocytes and (v) two-dimensional PAGE variants of seven proteins. Amongst the clones there was variation involving each of these five characters. At least seven different types of clones were found in ten cultures produced by dilution. The amount of phenotypic variation within a single isolate has thus been shown to be surprisingly great. Variations in drug susceptibility and antigens are considered to be particularly important in view of their relevance to anti-malarial treatments. |
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Thaithong, SodsriBeale, G. HFenton, BMcBride, JRosário, Virgílio E. doWalker, AWalliker, D2017-09-28T15:28:25Z2017-09-28T15:28:25Z1984THAITHONG, Sodsri et al. Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparumv. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 78, n. 2, p. 242-245, 1984.0035-9203https://patua.iec.gov.br/handle/iec/277610.1016/0035-9203(84)90287-6Clones of an isolate of Plasmodium falciparum from Mae Sod (Thailand) were prepared by a dilution procedure. Some of the parasite cultures thus obtained have been typed for the following characters: (i) electrophoretic variants of three enzymes; (ii) susceptibility to chloroquine and pyrimethamine; (iii) antigen diversities recognized by ten strain-specific monoclonal antibodies; (iv) presence or absence of knobs on infected erythrocytes and (v) two-dimensional PAGE variants of seven proteins. Amongst the clones there was variation involving each of these five characters. At least seven different types of clones were found in ten cultures produced by dilution. The amount of phenotypic variation within a single isolate has thus been shown to be surprisingly great. Variations in drug susceptibility and antigens are considered to be particularly important in view of their relevance to anti-malarial treatments.This work was supported by grants from the Wellcome Trust, UNDP/World Bank/WHO Special Programme, and Institute of Health Research, Chulalongkom University. We also thank Richard Fawcett for technical assistanceChulalongkorn University. Biology Department. Bangkok, Thailand.University of Edinburgh Scotland. Genetics Department. South Bridge, Edinburgh, Reino Unido.University of Edinburgh Scotland. Genetics Department. South Bridge, Edinburgh, Reino UnidoUniversity of Edinburgh. Department of Zoology. South Bridge, Edinburgh, Reino Unido.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.University of Edinburgh. Genetics Department. South Bridge, Edinburgh, Reino Unido.University of Edinburgh. Genetics Department. South Bridge, Edinburgh, Reino Unido.engOxford University PressClonal diversity in a single isolate of the malaria parasite Plasmodium falciparuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlasmodium falciparum / classificaçãoPlasmodium falciparum / enzimologiaPlasmodium falciparum / genéticaCélulas Clonais / classificaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdfClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdfapplication/pdf704945https://patua.iec.gov.br/bitstreams/064fe2a4-4240-4f39-a565-99d095287704/downloadaef825413e0fa85476c25b6f3274d277MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/e6466b2c-02c3-47b4-80d9-a1e450a25a2c/download52f1732ea66fbd1123abe39f5373b797MD52TEXTClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdf.txtClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdf.txtExtracted texttext/plain18975https://patua.iec.gov.br/bitstreams/a58749ec-955f-4c90-90d9-da5d80663e59/download430dc790ac3cc8b69f7ac6f0386b874fMD55THUMBNAILClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdf.jpgClonal diversity in a single isolate of the malaria parasite Plasmodium falciparum.pdf.jpgGenerated Thumbnailimage/jpeg6973https://patua.iec.gov.br/bitstreams/96573347-52d5-44b8-acf7-a18b7b8756ca/download97cb244be0bf23eea5345f25e6edcc73MD56iec/27762022-10-21 00:34:33.18oai:patua.iec.gov.br:iec/2776https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:34:33Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
title |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
spellingShingle |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum Thaithong, Sodsri Plasmodium falciparum / classificação Plasmodium falciparum / enzimologia Plasmodium falciparum / genética Células Clonais / classificação |
title_short |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
title_full |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
title_fullStr |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
title_full_unstemmed |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
title_sort |
Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum |
author |
Thaithong, Sodsri |
author_facet |
Thaithong, Sodsri Beale, G. H Fenton, B McBride, J Rosário, Virgílio E. do Walker, A Walliker, D |
author_role |
author |
author2 |
Beale, G. H Fenton, B McBride, J Rosário, Virgílio E. do Walker, A Walliker, D |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Thaithong, Sodsri Beale, G. H Fenton, B McBride, J Rosário, Virgílio E. do Walker, A Walliker, D |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Plasmodium falciparum / classificação Plasmodium falciparum / enzimologia Plasmodium falciparum / genética Células Clonais / classificação |
topic |
Plasmodium falciparum / classificação Plasmodium falciparum / enzimologia Plasmodium falciparum / genética Células Clonais / classificação |
description |
Clones of an isolate of Plasmodium falciparum from Mae Sod (Thailand) were prepared by a dilution procedure. Some of the parasite cultures thus obtained have been typed for the following characters: (i) electrophoretic variants of three enzymes; (ii) susceptibility to chloroquine and pyrimethamine; (iii) antigen diversities recognized by ten strain-specific monoclonal antibodies; (iv) presence or absence of knobs on infected erythrocytes and (v) two-dimensional PAGE variants of seven proteins. Amongst the clones there was variation involving each of these five characters. At least seven different types of clones were found in ten cultures produced by dilution. The amount of phenotypic variation within a single isolate has thus been shown to be surprisingly great. Variations in drug susceptibility and antigens are considered to be particularly important in view of their relevance to anti-malarial treatments. |
publishDate |
1984 |
dc.date.issued.fl_str_mv |
1984 |
dc.date.accessioned.fl_str_mv |
2017-09-28T15:28:25Z |
dc.date.available.fl_str_mv |
2017-09-28T15:28:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
THAITHONG, Sodsri et al. Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparumv. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 78, n. 2, p. 242-245, 1984. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2776 |
dc.identifier.issn.-.fl_str_mv |
0035-9203 |
dc.identifier.doi.-.fl_str_mv |
10.1016/0035-9203(84)90287-6 |
identifier_str_mv |
THAITHONG, Sodsri et al. Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparumv. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 78, n. 2, p. 242-245, 1984. 0035-9203 10.1016/0035-9203(84)90287-6 |
url |
https://patua.iec.gov.br/handle/iec/2776 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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