Insights into Limnothrix sp. metabolism based on comparative genomics

Detalhes bibliográficos
Autor(a) principal: Lima, Alex Ranieri Jerônimo
Data de Publicação: 2018
Outros Autores: Siqueira, Andrei Santos, Vasconcelos, Janaina Mota de, Pereira, James Siqueira, Azevedo, Juliana Simão Nina de, Moraes, Pablo Henrique Gonçalves, Aguiar, Délia Cristina Figueira, Lima, Clayton Pereira Silva de, Vianez Júnior, João Lídio da Silva Gonçalves, Nunes, Márcio Roberto Teixeira, Xavier, Luciana Pereira, Dall’Agnol, Leonardo Teixeira, Gonçalves, Evonnildo Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3836
Resumo: Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.
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spelling Lima, Alex Ranieri JerônimoSiqueira, Andrei SantosVasconcelos, Janaina Mota dePereira, James SiqueiraAzevedo, Juliana Simão Nina deMoraes, Pablo Henrique GonçalvesAguiar, Délia Cristina FigueiraLima, Clayton Pereira Silva deVianez Júnior, João Lídio da Silva GonçalvesNunes, Márcio Roberto TeixeiraXavier, Luciana PereiraDall’Agnol, Leonardo TeixeiraGonçalves, Evonnildo Costa2019-08-14T16:58:55Z2019-08-14T16:58:55Z2018LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf.1664-302Xhttps://patua.iec.gov.br/handle/iec/383610.3389/fmicb.2018.02811Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.This work was funded by Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (grants 556851/2009-9 and 554321/2010-6) and Fundação Amazônia Paraense (FAPESPA) (grant ICAAF 006/2012). This work was financially supported through grants from CAPES (Coordination for the Improvement of Higher Education Personnel, Brazil), PROPESP/UFPA (Pró-Reitoria de Pesquisa e Pós-Graduação/Universidade Federal do Pará, Brazil), FAPEMA (Maranhão State Research Foundation, Brazil), and Instituto Evandro Chagas (IEC).Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal Rural da Amazônia - Campus de Capanema. Laboratório de Biodiversidade Molecular. Capanema, PA, Brazil.Universidade Federal Rural da Amazônia - Campus de Capanema. Laboratório de Biodiversidade Molecular. Capanema, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Biotecnologia de Enzimas e Biotransformações. Belém, PA, Brazil.Universidade Federal do Maranhão. Grupo de Pesquisa em Biodiversidade, Bioprospecção e Biotecnologia. São Luís, MA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. 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dc.title.pt_BR.fl_str_mv Insights into Limnothrix sp. metabolism based on comparative genomics
title Insights into Limnothrix sp. metabolism based on comparative genomics
spellingShingle Insights into Limnothrix sp. metabolism based on comparative genomics
Lima, Alex Ranieri Jerônimo
Cianobactérias / genética
Genoma / genética
Análise de Sequência de DNA
Limnothrix spp
Metabolismo / genética
Ecossistema Amazônico
Centrais Hidrelétricas
title_short Insights into Limnothrix sp. metabolism based on comparative genomics
title_full Insights into Limnothrix sp. metabolism based on comparative genomics
title_fullStr Insights into Limnothrix sp. metabolism based on comparative genomics
title_full_unstemmed Insights into Limnothrix sp. metabolism based on comparative genomics
title_sort Insights into Limnothrix sp. metabolism based on comparative genomics
author Lima, Alex Ranieri Jerônimo
author_facet Lima, Alex Ranieri Jerônimo
Siqueira, Andrei Santos
Vasconcelos, Janaina Mota de
Pereira, James Siqueira
Azevedo, Juliana Simão Nina de
Moraes, Pablo Henrique Gonçalves
Aguiar, Délia Cristina Figueira
Lima, Clayton Pereira Silva de
Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Márcio Roberto Teixeira
Xavier, Luciana Pereira
Dall’Agnol, Leonardo Teixeira
Gonçalves, Evonnildo Costa
author_role author
author2 Siqueira, Andrei Santos
Vasconcelos, Janaina Mota de
Pereira, James Siqueira
Azevedo, Juliana Simão Nina de
Moraes, Pablo Henrique Gonçalves
Aguiar, Délia Cristina Figueira
Lima, Clayton Pereira Silva de
Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Márcio Roberto Teixeira
Xavier, Luciana Pereira
Dall’Agnol, Leonardo Teixeira
Gonçalves, Evonnildo Costa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Alex Ranieri Jerônimo
Siqueira, Andrei Santos
Vasconcelos, Janaina Mota de
Pereira, James Siqueira
Azevedo, Juliana Simão Nina de
Moraes, Pablo Henrique Gonçalves
Aguiar, Délia Cristina Figueira
Lima, Clayton Pereira Silva de
Vianez Júnior, João Lídio da Silva Gonçalves
Nunes, Márcio Roberto Teixeira
Xavier, Luciana Pereira
Dall’Agnol, Leonardo Teixeira
Gonçalves, Evonnildo Costa
dc.subject.decsPrimary.pt_BR.fl_str_mv Cianobactérias / genética
Genoma / genética
Análise de Sequência de DNA
Limnothrix spp
Metabolismo / genética
Ecossistema Amazônico
Centrais Hidrelétricas
topic Cianobactérias / genética
Genoma / genética
Análise de Sequência de DNA
Limnothrix spp
Metabolismo / genética
Ecossistema Amazônico
Centrais Hidrelétricas
description Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-08-14T16:58:55Z
dc.date.available.fl_str_mv 2019-08-14T16:58:55Z
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dc.identifier.citation.fl_str_mv LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3836
dc.identifier.issn.-.fl_str_mv 1664-302X
dc.identifier.doi.pt_BR.fl_str_mv 10.3389/fmicb.2018.02811
identifier_str_mv LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf.
1664-302X
10.3389/fmicb.2018.02811
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