Insights into Limnothrix sp. metabolism based on comparative genomics
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3836 |
Resumo: | Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism. |
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Lima, Alex Ranieri JerônimoSiqueira, Andrei SantosVasconcelos, Janaina Mota dePereira, James SiqueiraAzevedo, Juliana Simão Nina deMoraes, Pablo Henrique GonçalvesAguiar, Délia Cristina FigueiraLima, Clayton Pereira Silva deVianez Júnior, João Lídio da Silva GonçalvesNunes, Márcio Roberto TeixeiraXavier, Luciana PereiraDall’Agnol, Leonardo TeixeiraGonçalves, Evonnildo Costa2019-08-14T16:58:55Z2019-08-14T16:58:55Z2018LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf.1664-302Xhttps://patua.iec.gov.br/handle/iec/383610.3389/fmicb.2018.02811Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.This work was funded by Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (grants 556851/2009-9 and 554321/2010-6) and Fundação Amazônia Paraense (FAPESPA) (grant ICAAF 006/2012). This work was financially supported through grants from CAPES (Coordination for the Improvement of Higher Education Personnel, Brazil), PROPESP/UFPA (Pró-Reitoria de Pesquisa e Pós-Graduação/Universidade Federal do Pará, Brazil), FAPEMA (Maranhão State Research Foundation, Brazil), and Instituto Evandro Chagas (IEC).Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal Rural da Amazônia - Campus de Capanema. Laboratório de Biodiversidade Molecular. Capanema, PA, Brazil.Universidade Federal Rural da Amazônia - Campus de Capanema. Laboratório de Biodiversidade Molecular. Capanema, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Biotecnologia de Enzimas e Biotransformações. Belém, PA, Brazil.Universidade Federal do Maranhão. Grupo de Pesquisa em Biodiversidade, Bioprospecção e Biotecnologia. São Luís, MA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular. Belém, PA, Brazil.engFrontiers MediaInsights into Limnothrix sp. metabolism based on comparative genomicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCianobactérias / genéticaGenoma / genéticaAnálise de Sequência de DNALimnothrix sppMetabolismo / genéticaEcossistema AmazônicoCentrais Hidrelétricasinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALInsights into Limnothrix sp. metabolism based on comparative genomics.pdfInsights into Limnothrix sp. metabolism based on comparative genomics.pdfapplication/pdf7491049https://patua.iec.gov.br/bitstreams/002901df-ebd8-41d7-8fc7-4894ca92b672/download91bcb8e3c9910be09ff783bf17a227b9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/671fcb55-ba6f-40b7-b0c9-8ec799d619f6/download11832eea31b16df8613079d742d61793MD52TEXTInsights into Limnothrix sp. metabolism based on comparative genomics.pdf.txtInsights into Limnothrix sp. metabolism based on comparative genomics.pdf.txtExtracted texttext/plain89940https://patua.iec.gov.br/bitstreams/b1623573-6711-4bbc-90eb-37ff8b55f6cb/download6d63ca9b325f6cb9dadd5d5e34c1c18eMD55THUMBNAILInsights into Limnothrix sp. metabolism based on comparative genomics.pdf.jpgInsights into Limnothrix sp. metabolism based on comparative genomics.pdf.jpgGenerated Thumbnailimage/jpeg5568https://patua.iec.gov.br/bitstreams/9bd00c5d-3636-41ae-a07d-e83a8aef531c/download97ffc6865f6f93fe2db677c6043f06d3MD56iec/38362022-10-20 23:51:59.293oai:patua.iec.gov.br:iec/3836https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:51:59Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falsePGI+TGljZW7Dp2EgZGUgRGlzdHJpYnVpw6fDo28gTsOjbyBFeGNsdXNpdmE8L2I+DQoNClRvZG8gZGVwb3NpdGFudGUgZGUgbWF0ZXJpYWwgbm8gUGF0dcOhIGRldmUgY29uY2VkZXIgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIHVtYSBMaWNlbsOnYSBkZSBEaXN0cmlidWnDp8OjbyBOw6NvIEV4Y2x1c2l2YSBwYXJhIHRvcm5hciBlIG1hbnRlciBhY2Vzc8OtdmVpcyBvcyBzZXVzIGRvY3VtZW50b3MgZW0gZm9ybWF0byBkaWdpdGFsLiANCg0KQ29tIGEgY29uY2Vzc8OjbyBkZXNzYSBsaWNlbsOnYSBuw6NvIGV4Y2x1c2l2YSwgbyBkZXBvc2l0YW50ZSBjb250aW51YSBhIHJldGVyIHRvZG9zIG9zIGRpcmVpdG9zIGRlIGF1dG9yLg0KDQpBbyBhc3NpbmFyIGUgZW50cmVnYXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKQ0KDQphKSBEZWNsYXJhIHF1ZSBjb25oZWNlIGEgcG9sw610aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRlIHNldSBkb2N1bWVudG8uDQoNCmIpIENvbmNlZGUgYW8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIG8gZGlyZWl0byBuw6NvIGV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyIGUvb3UgZGlzdHJpYnVpciBubyA8Yj5QYXR1w6E8L2I+LCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgcG9yIHF1YWxxdWVyIG91dHJvIG1laW8uDQoNCmMpIERlY2xhcmEgcXVlIGF1dG9yaXphIG8gSW5zdGl0dXRvIEV2YW5kcm8gQ2hhZ2FzIGEgYXJxdWl2YXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRlc3NlIGRvY3VtZW50byBlIGNvbnZlcnTDqi1sbywgc2VtIGFsdGVyYXIgbyBzZXUgY29udGXDumRvLCBwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgZmljaGVpcm8sIG1laW8gb3Ugc3Vwb3J0ZSwgcGFyYSBlZmVpdG9zIGRlIHNlZ3VyYW7Dp2EsIHByZXNlcnZhw6fDo28gKGJhY2t1cCkgZSBhY2Vzc28uDQoNCmQpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCBlIHF1ZSBkZXTDqW0gbyBkaXJlaXRvIGRlIGNvbmNlZGVyIGEgdGVyY2Vpcm9zIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuDQoNCmUpIERlY2xhcmEgcXVlLCBubyBjYXNvIGRvIGRvY3VtZW50byBzdWJtZXRpZG8gY29udGVyIG1hdGVyaWFsIGRvIHF1YWwgbsOjbyBkZXTDqW0gb3MgZGlyZWl0b3MgZGUgYXV0b3IsIG9idGV2ZSBhIGF1dG9yaXphw6fDo28gaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhbyBJbnN0aXR1dG8gRXZhbmRybyBDaGFnYXMgb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIHV0aWxpesOhLWxvcyBsZWdhbG1lbnRlLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZSB0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBjb250ZcO6ZG8gZG8gZG9jdW1lbnRvIGVudHJlZ3VlLg0KDQpmKSBTRSBPIERPQ1VNRU5UTyBFTlRSRUdVRSBGT1IgQkFTRUFETyBFTSBUUkFCQUxITyBGSU5BTkNJQURPIE9VIEFQT0lBRE8gUE9SIE9VVFJBIElOU1RJVFVJw4fDg08gUVVFIE7Dg08gTyBJTlNUSVRVVE8gRVZBTkRSTyBDSEFHQVMsIERFQ0xBUkEgUVVFIENVTVBSSVUgUVVBSVNRVUVSIE9CUklHQcOHw5VFUyBFWElHSURBUyBQRUxPIFJFU1BFQ1RJVk8gQ09OVFJBVE8gT1UgQUNPUkRPLg0KDQpPIEluc3RpdHV0byBFdmFuZHJvIENoYWdhcyBpZGVudGlmaWNhcsOhIGNsYXJhbWVudGUgbyhzKSBub21lKHMpIGRvKHMpIGF1dG9yKGVzKSBkb3MgZGlyZWl0b3MgZG8gZG9jdW1lbnRvIGVudHJlZ3VlIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbyBhbMOpbSBkbyBwcmV2aXN0byBuYSBhbMOtbmVhIGIpLg== |
dc.title.pt_BR.fl_str_mv |
Insights into Limnothrix sp. metabolism based on comparative genomics |
title |
Insights into Limnothrix sp. metabolism based on comparative genomics |
spellingShingle |
Insights into Limnothrix sp. metabolism based on comparative genomics Lima, Alex Ranieri Jerônimo Cianobactérias / genética Genoma / genética Análise de Sequência de DNA Limnothrix spp Metabolismo / genética Ecossistema Amazônico Centrais Hidrelétricas |
title_short |
Insights into Limnothrix sp. metabolism based on comparative genomics |
title_full |
Insights into Limnothrix sp. metabolism based on comparative genomics |
title_fullStr |
Insights into Limnothrix sp. metabolism based on comparative genomics |
title_full_unstemmed |
Insights into Limnothrix sp. metabolism based on comparative genomics |
title_sort |
Insights into Limnothrix sp. metabolism based on comparative genomics |
author |
Lima, Alex Ranieri Jerônimo |
author_facet |
Lima, Alex Ranieri Jerônimo Siqueira, Andrei Santos Vasconcelos, Janaina Mota de Pereira, James Siqueira Azevedo, Juliana Simão Nina de Moraes, Pablo Henrique Gonçalves Aguiar, Délia Cristina Figueira Lima, Clayton Pereira Silva de Vianez Júnior, João Lídio da Silva Gonçalves Nunes, Márcio Roberto Teixeira Xavier, Luciana Pereira Dall’Agnol, Leonardo Teixeira Gonçalves, Evonnildo Costa |
author_role |
author |
author2 |
Siqueira, Andrei Santos Vasconcelos, Janaina Mota de Pereira, James Siqueira Azevedo, Juliana Simão Nina de Moraes, Pablo Henrique Gonçalves Aguiar, Délia Cristina Figueira Lima, Clayton Pereira Silva de Vianez Júnior, João Lídio da Silva Gonçalves Nunes, Márcio Roberto Teixeira Xavier, Luciana Pereira Dall’Agnol, Leonardo Teixeira Gonçalves, Evonnildo Costa |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Alex Ranieri Jerônimo Siqueira, Andrei Santos Vasconcelos, Janaina Mota de Pereira, James Siqueira Azevedo, Juliana Simão Nina de Moraes, Pablo Henrique Gonçalves Aguiar, Délia Cristina Figueira Lima, Clayton Pereira Silva de Vianez Júnior, João Lídio da Silva Gonçalves Nunes, Márcio Roberto Teixeira Xavier, Luciana Pereira Dall’Agnol, Leonardo Teixeira Gonçalves, Evonnildo Costa |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Cianobactérias / genética Genoma / genética Análise de Sequência de DNA Limnothrix spp Metabolismo / genética Ecossistema Amazônico Centrais Hidrelétricas |
topic |
Cianobactérias / genética Genoma / genética Análise de Sequência de DNA Limnothrix spp Metabolismo / genética Ecossistema Amazônico Centrais Hidrelétricas |
description |
Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-08-14T16:58:55Z |
dc.date.available.fl_str_mv |
2019-08-14T16:58:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3836 |
dc.identifier.issn.-.fl_str_mv |
1664-302X |
dc.identifier.doi.pt_BR.fl_str_mv |
10.3389/fmicb.2018.02811 |
identifier_str_mv |
LIMA, Alex Ranieri Jerônimo et al. Insights into Limnothrix sp. metabolism based on comparative genomics. Frontiers in Microbiology, v.9, n. 2811, Nov. 2018. DOI: https://doi.org/10.3389/fmicb.2018.02811. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6256058/pdf/fmicb-09-02811.pdf. 1664-302X 10.3389/fmicb.2018.02811 |
url |
https://patua.iec.gov.br/handle/iec/3836 |
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eng |
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eng |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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