Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil.

• Autor(a) principal: Pereira, H. G
• Data de Publicação: 1983
• Outros Autores: Azeredo, Rosete S, Leite, J. P. G, Candeias, J. A. N, Rácz, Maria L, Linhares, Alexandre da Costa, Gabbay, Yvone Benchimol, Trabulsi, J. R
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2731
• topic: Rotavírus; Eletroforese / métodos; Ensaio de Imunoadsorção Enzimática / métodos; Gastroenterite / microbiologia; Rotavírus / classificação; Rotavírus / genética; Rotavírus / isolamento e purificação; Infecções por Rotavírus / microbiologia; Rio de Janeiro (RJ); São Paulo (SP); Pará (PA)

Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86.8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positive samples, 18 (13.0%) were classified as subgroup 1, 94 (68.1%) as subgroup 2, and 26 (18.8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretic pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories.