Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes

Detalhes bibliográficos
Autor(a) principal: Silva, David S. da
Data de Publicação: 2021
Outros Autores: Silva Filho, Heriberto F. da, Cioffi, Marcelo B, Oliveira, Edivaldo Herculano Corrêa de, Gomes, Anderson J. B
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4309
Resumo: With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.
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spelling Silva, David S. daSilva Filho, Heriberto F. daCioffi, Marcelo BOliveira, Edivaldo Herculano Corrêa deGomes, Anderson J. B2021-05-24T13:05:43Z2021-05-24T13:05:43Z2021SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021.1424-859Xhttps://patua.iec.gov.br/handle/iec/430910.1159/000515831With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.Instituto Federal do Pará - Abaetetuba. Laboratório de Biologia Molecular, Evolução e Microbiologia. Abaetetuba, PA, Brazil.Universidade Federal do Pará. Laboratório de Biologia Celular e Helmintologia. Belém, PA, Brazil.Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto Ciência Exatas Naturais. Belém, PA, Brazil.Instituto Federal do Pará - Abaetetuba. Laboratório de Biologia Molecular, Evolução e Microbiologia. Abaetetuba, PA, Brazil.engKarger PublishersComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnfíbios / anatomia & histologiaCariótipoMarcadores Genéticos / genéticaHeterocromatina / genéticaRegião Organizadora do Nucléolo / genéticaAnálise CitogenéticaRepetições de Microssatélites / genéticaSequências Repetitivas de Ácido Nucleico / genéticainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdfComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/b85a352c-b7e8-4c29-8489-fd8fbca691dd/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/f812d79b-5db0-494c-bce4-20f2c27ca66d/download11832eea31b16df8613079d742d61793MD52TEXTComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.txtComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/e93bb25c-e06d-44fb-8dc4-21bd0fb952b4/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.jpgComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/bd20d26d-b26b-49e9-91f9-3a3ce64707f1/download71859d578212107f7f8c49a4ce09d9eeMD56iec/43092022-10-20 21:23:24.483oai:patua.iec.gov.br:iec/4309https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:23:24Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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
dc.title.pt_BR.fl_str_mv Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
title Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
spellingShingle Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
Silva, David S. da
Anfíbios / anatomia & histologia
Cariótipo
Marcadores Genéticos / genética
Heterocromatina / genética
Região Organizadora do Nucléolo / genética
Análise Citogenética
Repetições de Microssatélites / genética
Sequências Repetitivas de Ácido Nucleico / genética
title_short Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
title_full Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
title_fullStr Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
title_full_unstemmed Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
title_sort Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
author Silva, David S. da
author_facet Silva, David S. da
Silva Filho, Heriberto F. da
Cioffi, Marcelo B
Oliveira, Edivaldo Herculano Corrêa de
Gomes, Anderson J. B
author_role author
author2 Silva Filho, Heriberto F. da
Cioffi, Marcelo B
Oliveira, Edivaldo Herculano Corrêa de
Gomes, Anderson J. B
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, David S. da
Silva Filho, Heriberto F. da
Cioffi, Marcelo B
Oliveira, Edivaldo Herculano Corrêa de
Gomes, Anderson J. B
dc.subject.decsPrimary.pt_BR.fl_str_mv Anfíbios / anatomia & histologia
Cariótipo
Marcadores Genéticos / genética
Heterocromatina / genética
Região Organizadora do Nucléolo / genética
Análise Citogenética
Repetições de Microssatélites / genética
Sequências Repetitivas de Ácido Nucleico / genética
topic Anfíbios / anatomia & histologia
Cariótipo
Marcadores Genéticos / genética
Heterocromatina / genética
Região Organizadora do Nucléolo / genética
Análise Citogenética
Repetições de Microssatélites / genética
Sequências Repetitivas de Ácido Nucleico / genética
description With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-24T13:05:43Z
dc.date.available.fl_str_mv 2021-05-24T13:05:43Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4309
dc.identifier.issn.-.fl_str_mv 1424-859X
dc.identifier.doi.-.fl_str_mv 10.1159/000515831
identifier_str_mv SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021.
1424-859X
10.1159/000515831
url https://patua.iec.gov.br/handle/iec/4309
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Karger Publishers
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