Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4309 |
Resumo: | With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences. |
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Silva, David S. daSilva Filho, Heriberto F. daCioffi, Marcelo BOliveira, Edivaldo Herculano Corrêa deGomes, Anderson J. B2021-05-24T13:05:43Z2021-05-24T13:05:43Z2021SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021.1424-859Xhttps://patua.iec.gov.br/handle/iec/430910.1159/000515831With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.Instituto Federal do Pará - Abaetetuba. Laboratório de Biologia Molecular, Evolução e Microbiologia. Abaetetuba, PA, Brazil.Universidade Federal do Pará. Laboratório de Biologia Celular e Helmintologia. Belém, PA, Brazil.Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto Ciência Exatas Naturais. Belém, PA, Brazil.Instituto Federal do Pará - Abaetetuba. Laboratório de Biologia Molecular, Evolução e Microbiologia. Abaetetuba, PA, Brazil.engKarger PublishersComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnfíbios / anatomia & histologiaCariótipoMarcadores Genéticos / genéticaHeterocromatina / genéticaRegião Organizadora do Nucléolo / genéticaAnálise CitogenéticaRepetições de Microssatélites / genéticaSequências Repetitivas de Ácido Nucleico / genéticainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdfComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/b85a352c-b7e8-4c29-8489-fd8fbca691dd/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/f812d79b-5db0-494c-bce4-20f2c27ca66d/download11832eea31b16df8613079d742d61793MD52TEXTComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.txtComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/e93bb25c-e06d-44fb-8dc4-21bd0fb952b4/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55THUMBNAILComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.jpgComparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/bd20d26d-b26b-49e9-91f9-3a3ce64707f1/download71859d578212107f7f8c49a4ce09d9eeMD56iec/43092022-10-20 21:23:24.483oai:patua.iec.gov.br:iec/4309https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:23:24Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
title |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
spellingShingle |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes Silva, David S. da Anfíbios / anatomia & histologia Cariótipo Marcadores Genéticos / genética Heterocromatina / genética Região Organizadora do Nucléolo / genética Análise Citogenética Repetições de Microssatélites / genética Sequências Repetitivas de Ácido Nucleico / genética |
title_short |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
title_full |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
title_fullStr |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
title_full_unstemmed |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
title_sort |
Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes |
author |
Silva, David S. da |
author_facet |
Silva, David S. da Silva Filho, Heriberto F. da Cioffi, Marcelo B Oliveira, Edivaldo Herculano Corrêa de Gomes, Anderson J. B |
author_role |
author |
author2 |
Silva Filho, Heriberto F. da Cioffi, Marcelo B Oliveira, Edivaldo Herculano Corrêa de Gomes, Anderson J. B |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, David S. da Silva Filho, Heriberto F. da Cioffi, Marcelo B Oliveira, Edivaldo Herculano Corrêa de Gomes, Anderson J. B |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Anfíbios / anatomia & histologia Cariótipo Marcadores Genéticos / genética Heterocromatina / genética Região Organizadora do Nucléolo / genética Análise Citogenética Repetições de Microssatélites / genética Sequências Repetitivas de Ácido Nucleico / genética |
topic |
Anfíbios / anatomia & histologia Cariótipo Marcadores Genéticos / genética Heterocromatina / genética Região Organizadora do Nucléolo / genética Análise Citogenética Repetições de Microssatélites / genética Sequências Repetitivas de Ácido Nucleico / genética |
description |
With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-24T13:05:43Z |
dc.date.available.fl_str_mv |
2021-05-24T13:05:43Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4309 |
dc.identifier.issn.-.fl_str_mv |
1424-859X |
dc.identifier.doi.-.fl_str_mv |
10.1159/000515831 |
identifier_str_mv |
SILVA, David S. da et al. Comparative cytogenetics in four Leptodactylus species (Amphibia, Anura, Leptodactylidae): evidence of inner chromosomal diversification in highly conserved karyotypes. Cytogenetic and Genome Research, v. 161, n. 1/2, May 2021. 1424-859X 10.1159/000515831 |
url |
https://patua.iec.gov.br/handle/iec/4309 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.publisher.none.fl_str_mv |
Karger Publishers |
publisher.none.fl_str_mv |
Karger Publishers |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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