Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3592 |
Resumo: | This study's purpose was to identify polymorphisms (SNP) in the goat β-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. |
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Maia, Flora Suzane ParenteCampelo, José Elivalto GuimarãesSarmento, José Lindemberg RochaSilva, Caio SantosMarques, José Ribamar FelipeAlves, Francisco Arimatéia dos SantosGuimarães, Rafaelle CassebSilva Filho, Ednaldo2019-02-06T17:32:00Z2019-02-06T17:32:00Z2018MAIA, Flora Suzane Parente et al. Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat. Parasite Immunology, v. xx. e12613, n. xx, p. xx, Dec. 2018.(Epub ahead of prin)0141-9838https://patua.iec.gov.br/handle/iec/359210.1111/pim.12613This study's purpose was to identify polymorphisms (SNP) in the goat β-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections.Federal University of Piauí. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil.Federal University of Piauí. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil.Federal University of Piauí. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil.Embrapa Eastern Amazon. Brazilian Agricultural Research Corporation. Belém, PA, Brazil.Embrapa Eastern Amazon. Brazilian Agricultural Research Corporation. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Federal Rural University of Amazon. Health and Animal Production Institute. Belém, PA, Brazil.Federal Rural University of Amazon. Health and Animal Production Institute. Belém, PA, Brazil.engWileyAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goatinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePolimorfismo Genético / genéticaInfecções por NematoidesInfecções por ProtozoáriosCabras / parasitologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdfAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdfapplication/pdf606869https://patua.iec.gov.br/bitstreams/fc25159c-0109-4fca-a748-a1a93e3b8606/download75ab4ee27c5228b186a77184166372d1MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/1e871740-5d56-4d15-84fc-47ecfc6cf316/download11832eea31b16df8613079d742d61793MD52TEXTAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdf.txtAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdf.txtExtracted texttext/plain29310https://patua.iec.gov.br/bitstreams/ddcb7a64-04f1-45de-9777-00abf072acfa/download66a05064e380f611c26eabc1b1cc1e9fMD57THUMBNAILAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdf.jpgAssociation of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat.pdf.jpgGenerated Thumbnailimage/jpeg5967https://patua.iec.gov.br/bitstreams/f8f36e4e-8941-4737-8689-b0221161e2a5/downloadebe9bf2ef35d4c53b4aa6bb89948ca21MD58iec/35922022-10-20 21:33:52.226oai:patua.iec.gov.br:iec/3592https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:33:52Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
title |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
spellingShingle |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat Maia, Flora Suzane Parente Polimorfismo Genético / genética Infecções por Nematoides Infecções por Protozoários Cabras / parasitologia |
title_short |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
title_full |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
title_fullStr |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
title_full_unstemmed |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
title_sort |
Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat |
author |
Maia, Flora Suzane Parente |
author_facet |
Maia, Flora Suzane Parente Campelo, José Elivalto Guimarães Sarmento, José Lindemberg Rocha Silva, Caio Santos Marques, José Ribamar Felipe Alves, Francisco Arimatéia dos Santos Guimarães, Rafaelle Casseb Silva Filho, Ednaldo |
author_role |
author |
author2 |
Campelo, José Elivalto Guimarães Sarmento, José Lindemberg Rocha Silva, Caio Santos Marques, José Ribamar Felipe Alves, Francisco Arimatéia dos Santos Guimarães, Rafaelle Casseb Silva Filho, Ednaldo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Maia, Flora Suzane Parente Campelo, José Elivalto Guimarães Sarmento, José Lindemberg Rocha Silva, Caio Santos Marques, José Ribamar Felipe Alves, Francisco Arimatéia dos Santos Guimarães, Rafaelle Casseb Silva Filho, Ednaldo |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Polimorfismo Genético / genética Infecções por Nematoides Infecções por Protozoários Cabras / parasitologia |
topic |
Polimorfismo Genético / genética Infecções por Nematoides Infecções por Protozoários Cabras / parasitologia |
description |
This study's purpose was to identify polymorphisms (SNP) in the goat β-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha©). Polymorphism 2046, in turn, showed a significant association with Famacha© degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the β-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-02-06T17:32:00Z |
dc.date.available.fl_str_mv |
2019-02-06T17:32:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MAIA, Flora Suzane Parente et al. Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat. Parasite Immunology, v. xx. e12613, n. xx, p. xx, Dec. 2018.(Epub ahead of prin) |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3592 |
dc.identifier.issn.-.fl_str_mv |
0141-9838 |
dc.identifier.doi.-.fl_str_mv |
10.1111/pim.12613 |
identifier_str_mv |
MAIA, Flora Suzane Parente et al. Association of polymorphisms of the β-defensin 1 gene with nematode and protozoan infection traits in goat. Parasite Immunology, v. xx. e12613, n. xx, p. xx, Dec. 2018.(Epub ahead of prin) 0141-9838 10.1111/pim.12613 |
url |
https://patua.iec.gov.br/handle/iec/3592 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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