Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/384 |
Resumo: | We investigated whether the morphology of microglia in the molecular layer of the dentate gyrus (DG-Mol) or in the lacunosum molecular layer of CA1 (CA1-LMol) was correlated with spatial learning and memory in the capuchin monkey (Cebus apella). Learning and memory was tested in 4 monkeys with visuo-spatial, paired associated learning (PAL) tasks from the Cambridge battery of neuropsychological tests. After testing, monkeys were sacrificed, and hippocampi were sectioned. We specifically immunolabeled microglia with an antibody against the adapter binding, ionized calcium protein. Microglia were selected from the middle and outer thirds of the DG-Mol (n = 268) and the CA1-LMol (n = 185) for three-dimensional reconstructions created with Neurolucida and Neuroexplorer software. Cluster and discriminant analyses, based on microglial morphometric parameters, identified two major morphological microglia phenotypes (types I and II) found in both the CA1-LMol and DG-Mol of all individuals. Compared to type II, type I microglia were significantly smaller, thinner, more tortuous and ramified, and less complex (lower fractal dimensions). PAL performance was both linearly and nonlinearly correlated with type Imicroglial morphological features from the rostral and caudal DG-Mol, but not with microglia from the CA1-LMol. These differences in microglial morphology and correlations with PAL performance were consistent with previous proposals of hippocampal regional contributions for spatial learning and memory. Our results suggested that at least two morphological microglial phenotypes provided distinct physiological roles to learning-associated activity in the rostral and caudal DG-Mol of the monkey brain. |
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Santos Filho, CarlosLima, Camila M. deFôro, César A. ROliveira, Marcus A. deMagalhães, Nara G. MDiniz, Cristovam GuerreiroDiniz, Daniel GuerreiroVasconcelos, Pedro Fernando da CostaDiniz, Cristovam Wanderley Picanço2016-01-26T11:22:40Z2016-01-26T11:22:40Z2014SANTOS FILHO, Carlos et al. Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer. Journal of Chemical Neuroanatomy, v. 61/62, p. 176-188, Nov. 2014.1873-6300https://patua.iec.gov.br/handle/iec/38410.1016/j.jchemneu.2014.10.001We investigated whether the morphology of microglia in the molecular layer of the dentate gyrus (DG-Mol) or in the lacunosum molecular layer of CA1 (CA1-LMol) was correlated with spatial learning and memory in the capuchin monkey (Cebus apella). Learning and memory was tested in 4 monkeys with visuo-spatial, paired associated learning (PAL) tasks from the Cambridge battery of neuropsychological tests. After testing, monkeys were sacrificed, and hippocampi were sectioned. We specifically immunolabeled microglia with an antibody against the adapter binding, ionized calcium protein. Microglia were selected from the middle and outer thirds of the DG-Mol (n = 268) and the CA1-LMol (n = 185) for three-dimensional reconstructions created with Neurolucida and Neuroexplorer software. Cluster and discriminant analyses, based on microglial morphometric parameters, identified two major morphological microglia phenotypes (types I and II) found in both the CA1-LMol and DG-Mol of all individuals. Compared to type II, type I microglia were significantly smaller, thinner, more tortuous and ramified, and less complex (lower fractal dimensions). PAL performance was both linearly and nonlinearly correlated with type Imicroglial morphological features from the rostral and caudal DG-Mol, but not with microglia from the CA1-LMol. These differences in microglial morphology and correlations with PAL performance were consistent with previous proposals of hippocampal regional contributions for spatial learning and memory. Our results suggested that at least two morphological microglial phenotypes provided distinct physiological roles to learning-associated activity in the rostral and caudal DG-Mol of the monkey brain.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Universidade Federal do Pará - Instituto de Ciências Biológicas - Hospital Universitário João de Barros Barreto - Laboratório de Investigações em Neurodegeneração e Infecção, Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Hospital Universitário João de Barros Barreto. Laboratório de Investigações em Neurodegeneração e Infecção. 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dc.title.pt_BR.fl_str_mv |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
title |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
spellingShingle |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer Santos Filho, Carlos Microglia / citologia Giro Denteado Primatas Aprendizagem |
title_short |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
title_full |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
title_fullStr |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
title_full_unstemmed |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
title_sort |
Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer |
author |
Santos Filho, Carlos |
author_facet |
Santos Filho, Carlos Lima, Camila M. de Fôro, César A. R Oliveira, Marcus A. de Magalhães, Nara G. M Diniz, Cristovam Guerreiro Diniz, Daniel Guerreiro Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author_role |
author |
author2 |
Lima, Camila M. de Fôro, César A. R Oliveira, Marcus A. de Magalhães, Nara G. M Diniz, Cristovam Guerreiro Diniz, Daniel Guerreiro Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos Filho, Carlos Lima, Camila M. de Fôro, César A. R Oliveira, Marcus A. de Magalhães, Nara G. M Diniz, Cristovam Guerreiro Diniz, Daniel Guerreiro Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Microglia / citologia Giro Denteado Primatas Aprendizagem |
topic |
Microglia / citologia Giro Denteado Primatas Aprendizagem |
description |
We investigated whether the morphology of microglia in the molecular layer of the dentate gyrus (DG-Mol) or in the lacunosum molecular layer of CA1 (CA1-LMol) was correlated with spatial learning and memory in the capuchin monkey (Cebus apella). Learning and memory was tested in 4 monkeys with visuo-spatial, paired associated learning (PAL) tasks from the Cambridge battery of neuropsychological tests. After testing, monkeys were sacrificed, and hippocampi were sectioned. We specifically immunolabeled microglia with an antibody against the adapter binding, ionized calcium protein. Microglia were selected from the middle and outer thirds of the DG-Mol (n = 268) and the CA1-LMol (n = 185) for three-dimensional reconstructions created with Neurolucida and Neuroexplorer software. Cluster and discriminant analyses, based on microglial morphometric parameters, identified two major morphological microglia phenotypes (types I and II) found in both the CA1-LMol and DG-Mol of all individuals. Compared to type II, type I microglia were significantly smaller, thinner, more tortuous and ramified, and less complex (lower fractal dimensions). PAL performance was both linearly and nonlinearly correlated with type Imicroglial morphological features from the rostral and caudal DG-Mol, but not with microglia from the CA1-LMol. These differences in microglial morphology and correlations with PAL performance were consistent with previous proposals of hippocampal regional contributions for spatial learning and memory. Our results suggested that at least two morphological microglial phenotypes provided distinct physiological roles to learning-associated activity in the rostral and caudal DG-Mol of the monkey brain. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2016-01-26T11:22:40Z |
dc.date.available.fl_str_mv |
2016-01-26T11:22:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS FILHO, Carlos et al. Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer. Journal of Chemical Neuroanatomy, v. 61/62, p. 176-188, Nov. 2014. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/384 |
dc.identifier.issn.-.fl_str_mv |
1873-6300 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.jchemneu.2014.10.001 |
identifier_str_mv |
SANTOS FILHO, Carlos et al. Visuospatial learning and memory in the Cebus apella and microglial morphology in the molecular layer of the dentate gyrus and CA1 lacunosum molecular layer. Journal of Chemical Neuroanatomy, v. 61/62, p. 176-188, Nov. 2014. 1873-6300 10.1016/j.jchemneu.2014.10.001 |
url |
https://patua.iec.gov.br/handle/iec/384 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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