A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine

Detalhes bibliográficos
Autor(a) principal: Fonseca, Erica Lourenço
Data de Publicação: 2017
Outros Autores: Ramos, Nilceia da Veiga, Andrade, Bruno G. Nascimento, Morais, Lena Líllian Canto de Sá, Marin, Michel F. Abanto, Vicente, Ana Carolina P
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2879
Resumo: Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
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spelling Fonseca, Erica LourençoRamos, Nilceia da VeigaAndrade, Bruno G. NascimentoMorais, Lena Líllian Canto de SáMarin, Michel F. AbantoVicente, Ana Carolina P2017-11-24T14:14:08Z2017-11-24T14:14:08Z2017FONSECA, Erica Lourenço et al. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagnostic Microbiology and Infectious Disease, v. 87, n. 4, p. 315-317, Apr. 2017.0732-8893https://patua.iec.gov.br/handle/iec/287910.1016/j.diagmicrobio.2017.01.005Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.This work was supported by FAPERJ and PNPD-CAPES fellowships and CNPq grantFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.engElsevierA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleKlebsiella / genéticaInfecções por Klebsiella / microbiologiaKlebsiella pneumoniae / genéticabeta-Lactamases / genéticaDNA Bacteriano / genéticaTestes de Sensibilidade MicrobianaReação em Cadeia da Polimerase Multiplex / métodosClass A β-lactamaseIdentificação molecularinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/a1b9e385-f731-41f1-9f3c-2824e1e6defe/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdfA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdfapplication/pdf605645https://patua.iec.gov.br/bitstreams/3ba78fc6-74bc-471d-b8ad-8f3c787cf9d2/download18c203c206bf7e3a065a92c60024c96fMD53TEXTA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdf.txtA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdf.txtExtracted texttext/plain17723https://patua.iec.gov.br/bitstreams/1534ff3a-447d-4fe0-a922-408e1dc66a1d/download8ff76770dc229851fa4ca6044943e5e9MD56THUMBNAILA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdf.jpgA one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.pdf.jpgGenerated Thumbnailimage/jpeg5769https://patua.iec.gov.br/bitstreams/a5242883-9caa-42d7-814b-7996fd0403d0/download3014790d538881456847d400dadab865MD57iec/28792022-10-21 00:29:54.362oai:patua.iec.gov.br:iec/2879https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:29:54Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
title A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
spellingShingle A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
Fonseca, Erica Lourenço
Klebsiella / genética
Infecções por Klebsiella / microbiologia
Klebsiella pneumoniae / genética
beta-Lactamases / genética
DNA Bacteriano / genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase Multiplex / métodos
Class A β-lactamase
Identificação molecular
title_short A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
title_full A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
title_fullStr A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
title_full_unstemmed A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
title_sort A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
author Fonseca, Erica Lourenço
author_facet Fonseca, Erica Lourenço
Ramos, Nilceia da Veiga
Andrade, Bruno G. Nascimento
Morais, Lena Líllian Canto de Sá
Marin, Michel F. Abanto
Vicente, Ana Carolina P
author_role author
author2 Ramos, Nilceia da Veiga
Andrade, Bruno G. Nascimento
Morais, Lena Líllian Canto de Sá
Marin, Michel F. Abanto
Vicente, Ana Carolina P
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Erica Lourenço
Ramos, Nilceia da Veiga
Andrade, Bruno G. Nascimento
Morais, Lena Líllian Canto de Sá
Marin, Michel F. Abanto
Vicente, Ana Carolina P
dc.subject.decsPrimary.pt_BR.fl_str_mv Klebsiella / genética
Infecções por Klebsiella / microbiologia
Klebsiella pneumoniae / genética
beta-Lactamases / genética
DNA Bacteriano / genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase Multiplex / métodos
Class A β-lactamase
Identificação molecular
topic Klebsiella / genética
Infecções por Klebsiella / microbiologia
Klebsiella pneumoniae / genética
beta-Lactamases / genética
DNA Bacteriano / genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase Multiplex / métodos
Class A β-lactamase
Identificação molecular
description Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-24T14:14:08Z
dc.date.available.fl_str_mv 2017-11-24T14:14:08Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, Erica Lourenço et al. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagnostic Microbiology and Infectious Disease, v. 87, n. 4, p. 315-317, Apr. 2017.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2879
dc.identifier.issn.-.fl_str_mv 0732-8893
dc.identifier.doi.-.fl_str_mv 10.1016/j.diagmicrobio.2017.01.005
identifier_str_mv FONSECA, Erica Lourenço et al. A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagnostic Microbiology and Infectious Disease, v. 87, n. 4, p. 315-317, Apr. 2017.
0732-8893
10.1016/j.diagmicrobio.2017.01.005
url https://patua.iec.gov.br/handle/iec/2879
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
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