Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3659 |
Resumo: | The objective of the present work was to analyze the cytotoxic, antimicrobial activity and the action mechanism of the major component in of the Syzygium aromaticum essential oil obtained by supercritical CO 2 . In this work, gingival fibroblasts were exposed to the essential oil in different concentrations for one hour: 5 μL/ml, 7.5 μL/ml and 10 μL/ml. Culture medium was used as control. Cytotoxicity analysis was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ® ) method. The susceptibility was evaluated on three microorganisms Candida albicans, Escherichia coli and Staphylococcus aureus. The statistical analyses showed significant difference in cell viability for the concentration of 10 μL/mL, as compared to the control group. As a result, the plant extract had no cytotoxicity at concentrations below 10 μL/mL in human gingival fibroblasts. The interaction mode of eugenol, the major compound and main component responsible for the biological activity of the essential oil was evaluated. The molecular docking of eugenol with important proteins of the metabolic pathway of the microorganisms C. albicans, E. coli and S. aureus were performed. The results demonstrated that the compound is capable of interacting with catalytic residues of the enzymes and forming an energetically favorable system with such proteins. The results of binding free energy obtained demonstrate this capacity. For the eugenol-N-myristoyltransferase (C. albicans) system, the value of ∆G bind was −19.01 kcal/mol, for Enoyl reductase (E. Coli) ∆G bind was equal to −11.31 kcal/mol and for SarA (S. aureus) ∆G bind was −13.58 kcal/mol. |
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Oliveira, Mozaniel Santana deCruz, Jorddy Neves daMitre, Geovanni PereiraCosta, Wanessa Almeida daKataoka, Maria Sueli da SilvaSilva, Sebastião GomesAlves, Ana Cláudia Braga AmorasPinheiro, João de Jesus VianaSilva, Silvia Helena Marques daMenezes, Sílvio Augusto Fernandes deMenezes, Tatiany Oliveira de AlencarChaves Neto, Antônio Maia de JesusCarvalho Junior, Raul Nunes de2019-04-23T17:14:50Z2019-04-23T17:14:50Z2019OLIVEIRA, Mozaniel Santana de et al. Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent. Journal of Computational and Theoretical Nanoscience, v. 16, n. 2, p. 355-364, Feb. 2019. DOI: https://doi.org/10.1166/jctn.2019.81081546-1955https://patua.iec.gov.br/handle/iec/365910.1166/jctn.2019.8108The objective of the present work was to analyze the cytotoxic, antimicrobial activity and the action mechanism of the major component in of the Syzygium aromaticum essential oil obtained by supercritical CO 2 . In this work, gingival fibroblasts were exposed to the essential oil in different concentrations for one hour: 5 μL/ml, 7.5 μL/ml and 10 μL/ml. Culture medium was used as control. Cytotoxicity analysis was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ® ) method. The susceptibility was evaluated on three microorganisms Candida albicans, Escherichia coli and Staphylococcus aureus. The statistical analyses showed significant difference in cell viability for the concentration of 10 μL/mL, as compared to the control group. As a result, the plant extract had no cytotoxicity at concentrations below 10 μL/mL in human gingival fibroblasts. The interaction mode of eugenol, the major compound and main component responsible for the biological activity of the essential oil was evaluated. The molecular docking of eugenol with important proteins of the metabolic pathway of the microorganisms C. albicans, E. coli and S. aureus were performed. The results demonstrated that the compound is capable of interacting with catalytic residues of the enzymes and forming an energetically favorable system with such proteins. The results of binding free energy obtained demonstrate this capacity. For the eugenol-N-myristoyltransferase (C. albicans) system, the value of ∆G bind was −19.01 kcal/mol, for Enoyl reductase (E. Coli) ∆G bind was equal to −11.31 kcal/mol and for SarA (S. aureus) ∆G bind was −13.58 kcal/mol.Lambda Alpha International (1427204); Montana Tech (1662230);Federal University of Para. Faculty of Food Engineering. Program of Post-Graduation in Food Science and Technology. Belém, PA, Brazil.Federal University of Para. Laboratory of Preparation and Computation of Nanomaterials. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Cell Culture Laboratory. Belém, PA, Brazil.Federal University of Para. Program of Post-Graduation in Natural Resources Engineering. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Cell Culture Laboratory. Belém, PA, Brazil.Federal University of Para. Program of Post-Graduation in Chemistry. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Cell Culture Laboratory. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Cell Culture Laboratory. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.University Center of Pará State. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Cell Culture Laboratory. Belém, PA, Brazil.Federal University of Para. Laboratory of Preparation and Computation of Nanomaterials. Belém, PA, Brazil.Federal University of Para. Faculty of Food Engineering. Program of Post-Graduation in Food Science and Technology. Belém, PA, Brazil.engAmerican Scientific PublishersAntimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleÓleos Voláteis / químicaSyzygium / químicaEugenol / síntese químicaExtratos Vegetais / químicaTestes Imunológicos de Citotoxicidadeinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/71839182-ed66-4dd3-bd70-b9010888fc42/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/d9f8fdd9-57a4-4dd1-b255-857a08350259/download11832eea31b16df8613079d742d61793MD52TEXTAntimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent.pdf.txtAntimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/58a66022-62c3-47b0-afb2-e006f6c9f70b/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/c85bda0b-d27a-424c-a2dc-814e70509379/downloade1c06d85ae7b8b032bef47e42e4c08f9MD57THUMBNAILAntimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent.pdf.jpgAntimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/160fa030-256a-4c43-b2dd-aacd9cd63003/download71859d578212107f7f8c49a4ce09d9eeMD56Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/7168fcc8-67cc-47d8-9a07-22cf4e7921de/download71859d578212107f7f8c49a4ce09d9eeMD58iec/36592023-06-19 13:24:54.75oai:patua.iec.gov.br:iec/3659https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-19T13:24:54Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
title |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
spellingShingle |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent Oliveira, Mozaniel Santana de Óleos Voláteis / química Syzygium / química Eugenol / síntese química Extratos Vegetais / química Testes Imunológicos de Citotoxicidade |
title_short |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
title_full |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
title_fullStr |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
title_full_unstemmed |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
title_sort |
Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent |
author |
Oliveira, Mozaniel Santana de |
author_facet |
Oliveira, Mozaniel Santana de Cruz, Jorddy Neves da Mitre, Geovanni Pereira Costa, Wanessa Almeida da Kataoka, Maria Sueli da Silva Silva, Sebastião Gomes Alves, Ana Cláudia Braga Amoras Pinheiro, João de Jesus Viana Silva, Silvia Helena Marques da Menezes, Sílvio Augusto Fernandes de Menezes, Tatiany Oliveira de Alencar Chaves Neto, Antônio Maia de Jesus Carvalho Junior, Raul Nunes de |
author_role |
author |
author2 |
Cruz, Jorddy Neves da Mitre, Geovanni Pereira Costa, Wanessa Almeida da Kataoka, Maria Sueli da Silva Silva, Sebastião Gomes Alves, Ana Cláudia Braga Amoras Pinheiro, João de Jesus Viana Silva, Silvia Helena Marques da Menezes, Sílvio Augusto Fernandes de Menezes, Tatiany Oliveira de Alencar Chaves Neto, Antônio Maia de Jesus Carvalho Junior, Raul Nunes de |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Mozaniel Santana de Cruz, Jorddy Neves da Mitre, Geovanni Pereira Costa, Wanessa Almeida da Kataoka, Maria Sueli da Silva Silva, Sebastião Gomes Alves, Ana Cláudia Braga Amoras Pinheiro, João de Jesus Viana Silva, Silvia Helena Marques da Menezes, Sílvio Augusto Fernandes de Menezes, Tatiany Oliveira de Alencar Chaves Neto, Antônio Maia de Jesus Carvalho Junior, Raul Nunes de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Óleos Voláteis / química Syzygium / química Eugenol / síntese química Extratos Vegetais / química Testes Imunológicos de Citotoxicidade |
topic |
Óleos Voláteis / química Syzygium / química Eugenol / síntese química Extratos Vegetais / química Testes Imunológicos de Citotoxicidade |
description |
The objective of the present work was to analyze the cytotoxic, antimicrobial activity and the action mechanism of the major component in of the Syzygium aromaticum essential oil obtained by supercritical CO 2 . In this work, gingival fibroblasts were exposed to the essential oil in different concentrations for one hour: 5 μL/ml, 7.5 μL/ml and 10 μL/ml. Culture medium was used as control. Cytotoxicity analysis was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ® ) method. The susceptibility was evaluated on three microorganisms Candida albicans, Escherichia coli and Staphylococcus aureus. The statistical analyses showed significant difference in cell viability for the concentration of 10 μL/mL, as compared to the control group. As a result, the plant extract had no cytotoxicity at concentrations below 10 μL/mL in human gingival fibroblasts. The interaction mode of eugenol, the major compound and main component responsible for the biological activity of the essential oil was evaluated. The molecular docking of eugenol with important proteins of the metabolic pathway of the microorganisms C. albicans, E. coli and S. aureus were performed. The results demonstrated that the compound is capable of interacting with catalytic residues of the enzymes and forming an energetically favorable system with such proteins. The results of binding free energy obtained demonstrate this capacity. For the eugenol-N-myristoyltransferase (C. albicans) system, the value of ∆G bind was −19.01 kcal/mol, for Enoyl reductase (E. Coli) ∆G bind was equal to −11.31 kcal/mol and for SarA (S. aureus) ∆G bind was −13.58 kcal/mol. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-04-23T17:14:50Z |
dc.date.available.fl_str_mv |
2019-04-23T17:14:50Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Mozaniel Santana de et al. Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent. Journal of Computational and Theoretical Nanoscience, v. 16, n. 2, p. 355-364, Feb. 2019. DOI: https://doi.org/10.1166/jctn.2019.8108 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3659 |
dc.identifier.issn.-.fl_str_mv |
1546-1955 |
dc.identifier.doi.-.fl_str_mv |
10.1166/jctn.2019.8108 |
identifier_str_mv |
OLIVEIRA, Mozaniel Santana de et al. Antimicrobial, cytotoxic activity of the syzygium aromaticum essential oil, molecular docking and dynamics molecular studies of its major chemical constituent. Journal of Computational and Theoretical Nanoscience, v. 16, n. 2, p. 355-364, Feb. 2019. DOI: https://doi.org/10.1166/jctn.2019.8108 1546-1955 10.1166/jctn.2019.8108 |
url |
https://patua.iec.gov.br/handle/iec/3659 |
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eng |
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eng |
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American Scientific Publishers |
publisher.none.fl_str_mv |
American Scientific Publishers |
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