Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.

Detalhes bibliográficos
Autor(a) principal: Shi, Ya Ping
Data de Publicação: 1993
Outros Autores: Udhayakumar, Venkatachalam, Alpers, Michael P, Póvoa, Marinete Marins, Oloo, Aggrey J, Ruebush, Trenton K, Lal, Altaf A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2610
Resumo: Synthetic peptides and human serum or plasma samples from regions of Brazil, Papua New Guinea, and Kenya in which malaria is endemic were used to identify B-cell epitopes localized outside the repeat region of the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum. In agreement with recent observations, our results confirm the presence of two non-repeat-region-based B-cell epitopes of the CS protein. Of these two epitopes, only the region I epitope (KPKHKKLKQPGDGNP) was previously shown to be recognized by human sera. In this study, we show that human immune sera from malarious regions recognize another B-cell epitope, ENANANNAV, that resides carboxyl to the repeat region. The present study reveals that (i) the repeat-sequence (NANP)-based B-cell epitope of the CS protein is an immunogenic but not immunodominant epitope; (ii) the natural expression of antibody responses to the two non-repeat-region-based B-cell epitopes of the CS protein varies in different populations in which malaria is endemic; (iii) although the host immune responses to the non-repeat-region-based B-cell epitopes increase as a function of host age, this increase is not statistically significant for the region I epitope but is significant for the other epitope; and (iv) the Th1R T-cell site but not the Th2R or Th3R T-cell site induces an antibody response in the human host. This study confirms the immunogenic potential of non-repeat-region-based B-cell epitopes and suggests that antibody pressures may also contribute to the maintenance of the antigenic diversity of the CS protein.
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spelling Shi, Ya PingUdhayakumar, VenkatachalamAlpers, Michael PPóvoa, Marinete MarinsOloo, Aggrey JRuebush, Trenton KLal, Altaf A2017-06-12T18:46:11Z2017-06-12T18:46:11Z1993SHI, Ya Ping et al. Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein. Infection and Immunity, v. 61, n. 6. p. 2425-2433, June 1993.0019-9567https://patua.iec.gov.br/handle/iec/2610Synthetic peptides and human serum or plasma samples from regions of Brazil, Papua New Guinea, and Kenya in which malaria is endemic were used to identify B-cell epitopes localized outside the repeat region of the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum. In agreement with recent observations, our results confirm the presence of two non-repeat-region-based B-cell epitopes of the CS protein. Of these two epitopes, only the region I epitope (KPKHKKLKQPGDGNP) was previously shown to be recognized by human sera. In this study, we show that human immune sera from malarious regions recognize another B-cell epitope, ENANANNAV, that resides carboxyl to the repeat region. The present study reveals that (i) the repeat-sequence (NANP)-based B-cell epitope of the CS protein is an immunogenic but not immunodominant epitope; (ii) the natural expression of antibody responses to the two non-repeat-region-based B-cell epitopes of the CS protein varies in different populations in which malaria is endemic; (iii) although the host immune responses to the non-repeat-region-based B-cell epitopes increase as a function of host age, this increase is not statistically significant for the region I epitope but is significant for the other epitope; and (iv) the Th1R T-cell site but not the Th2R or Th3R T-cell site induces an antibody response in the human host. This study confirms the immunogenic potential of non-repeat-region-based B-cell epitopes and suggests that antibody pressures may also contribute to the maintenance of the antigenic diversity of the CS protein.Centers for Disease Control.Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta, Georgia, USA.Centers for Disease Control.Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta, Georgia, USA.Papua New Guinea Institute of Medical Research. Goroka, Papua New GuineaMinistério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Kenya Medical Research Institute. Vector Biology and Control Research Center. Kisumu, Kenya.Centers for Disease Control.Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta, Georgia, USA / Kenya Medical Research Institute. Vector Biology and Control Research Center. Kisumu, Kenya.Centers for Disease Control.Division of Parasitic Diseases, National Center for Infectious Diseases. Malaria Branch. Atlanta, Georgia, USA.engAmerican Society for MicrobiologyNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária Falciparum / diagnósticoPlasmodium falciparumMaláriaAnticorpos / sangueFormação de Anticorposinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdfNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdfapplication/pdf1699912https://patua.iec.gov.br/bitstreams/8339535e-2ba2-495d-8b4e-b65b94b21775/download3b6d9a96b8ae427b584763d84a974be0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/478654ff-aa67-46ce-834a-9a2fe7c4b68e/download52f1732ea66fbd1123abe39f5373b797MD52TEXTNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdf.txtNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdf.txtExtracted texttext/plain45986https://patua.iec.gov.br/bitstreams/65b721e0-be6b-41cb-a9e6-bfaf1aae778b/downloadf8da81ceee3b8ef12e8704bc5d86bc44MD55THUMBNAILNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdf.jpgNatural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.pdf.jpgGenerated Thumbnailimage/jpeg7371https://patua.iec.gov.br/bitstreams/5513a224-d8f6-49ac-a446-43010f4e552c/downloadde6fe6402a97af1500ba32a9a93e2e75MD56iec/26102022-10-21 00:15:30.091oai:patua.iec.gov.br:iec/2610https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:15:30Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
title Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
spellingShingle Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
Shi, Ya Ping
Malária Falciparum / diagnóstico
Plasmodium falciparum
Malária
Anticorpos / sangue
Formação de Anticorpos
title_short Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
title_full Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
title_fullStr Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
title_full_unstemmed Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
title_sort Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein.
author Shi, Ya Ping
author_facet Shi, Ya Ping
Udhayakumar, Venkatachalam
Alpers, Michael P
Póvoa, Marinete Marins
Oloo, Aggrey J
Ruebush, Trenton K
Lal, Altaf A
author_role author
author2 Udhayakumar, Venkatachalam
Alpers, Michael P
Póvoa, Marinete Marins
Oloo, Aggrey J
Ruebush, Trenton K
Lal, Altaf A
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Shi, Ya Ping
Udhayakumar, Venkatachalam
Alpers, Michael P
Póvoa, Marinete Marins
Oloo, Aggrey J
Ruebush, Trenton K
Lal, Altaf A
dc.subject.decsPrimary.pt_BR.fl_str_mv Malária Falciparum / diagnóstico
Plasmodium falciparum
Malária
Anticorpos / sangue
Formação de Anticorpos
topic Malária Falciparum / diagnóstico
Plasmodium falciparum
Malária
Anticorpos / sangue
Formação de Anticorpos
description Synthetic peptides and human serum or plasma samples from regions of Brazil, Papua New Guinea, and Kenya in which malaria is endemic were used to identify B-cell epitopes localized outside the repeat region of the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum. In agreement with recent observations, our results confirm the presence of two non-repeat-region-based B-cell epitopes of the CS protein. Of these two epitopes, only the region I epitope (KPKHKKLKQPGDGNP) was previously shown to be recognized by human sera. In this study, we show that human immune sera from malarious regions recognize another B-cell epitope, ENANANNAV, that resides carboxyl to the repeat region. The present study reveals that (i) the repeat-sequence (NANP)-based B-cell epitope of the CS protein is an immunogenic but not immunodominant epitope; (ii) the natural expression of antibody responses to the two non-repeat-region-based B-cell epitopes of the CS protein varies in different populations in which malaria is endemic; (iii) although the host immune responses to the non-repeat-region-based B-cell epitopes increase as a function of host age, this increase is not statistically significant for the region I epitope but is significant for the other epitope; and (iv) the Th1R T-cell site but not the Th2R or Th3R T-cell site induces an antibody response in the human host. This study confirms the immunogenic potential of non-repeat-region-based B-cell epitopes and suggests that antibody pressures may also contribute to the maintenance of the antigenic diversity of the CS protein.
publishDate 1993
dc.date.issued.fl_str_mv 1993
dc.date.accessioned.fl_str_mv 2017-06-12T18:46:11Z
dc.date.available.fl_str_mv 2017-06-12T18:46:11Z
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dc.identifier.citation.fl_str_mv SHI, Ya Ping et al. Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein. Infection and Immunity, v. 61, n. 6. p. 2425-2433, June 1993.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2610
dc.identifier.issn.-.fl_str_mv 0019-9567
identifier_str_mv SHI, Ya Ping et al. Natural antibody responses against the non-repeat-sequence-based B-cell epitopes of the Plasmodium falciparum circumsporozoite protein. Infection and Immunity, v. 61, n. 6. p. 2425-2433, June 1993.
0019-9567
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