Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime

• Autor(a) principal: Gélvez, Ana Patricia Cacua
• Data de Publicação: 2018
• Outros Autores: Farias, Luis Henrique Seabra, Pereira, Victor Soares, Silva, Inaldo Claudio Martins da, Costa, Ana Carolina, Dias, Carmen Gilda Barroso Tavares, Costa, Roseane Maria Ribeiro, Silva, Silvia Helena Marques da, Rodrigues, Ana Paula Drummond
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3064
• topic: Leishmania / efeitos de drogas; Nanopartículas / biossíntese; Leishmaniose Cutânea / tratamento farmacológico; Fármacos Dermatológicos; Tratamento Farmacológico

Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis. Materials & methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated. Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite1, nanocomposite2 and nanocomposite3, respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite2 and nanocomposite3, respectively. Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo.