Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4329 |
Resumo: | Background: Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods: Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 μM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 μM MeHg were tested by measuring glutathione (GSH) level. Results: It was observed that cell viability decreased significantly after exposure to 2.5 and 5 μM of MeHg, but the metabolic viability only decreased significantly at 5 μM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 μM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells. |
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Souza-Rodrigues, Renata Duarte dePuty, BrunaBonfim, LaísNogueira, Lygia SegaNascimento, Priscila CunhaBittencourt, Leonardo OliveiraCouto, Roberta Souza D'AlmeidaBarboza, Carlos Augusto GalvãoOliveira, Edivaldo Herculano Corrêa deMarques, Marcia MartinsLima, Rafael Rodrigues2021-06-21T14:33:59Z2021-06-21T14:33:59Z2021SOUZA-RODRIGUES, Renata Duarte de et al. Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ, v. 9, n. 11114, June 2021.2167-8359https://patua.iec.gov.br/handle/iec/432910.7717/peerj.11114Background: Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods: Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 μM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 μM MeHg were tested by measuring glutathione (GSH) level. Results: It was observed that cell viability decreased significantly after exposure to 2.5 and 5 μM of MeHg, but the metabolic viability only decreased significantly at 5 μM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 μM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells.Federal University of Pará. Institute of Arts Sciences. Belém, PA, Brazil / University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil..University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.Federal University of Pará. Faculty of Dentistry. Belém, PA, Brazil.Federal University of Rio Grande do Norte. Department of Morphology. Natal, RN, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.Ibirapuera University. School of Dentistry. Graduation Program. São Paulo, SP, Brazil.University of Pará. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Belém, PA, Brazil.engPeerJMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findingsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePolpa Dentária / patologiaCompostos de Metilmercúrio / toxicidadeAmálgama Dentário / toxicidadeEstresse Oxidativoinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTEXTMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdf.txtMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdf.txtExtracted texttext/plain58779https://patua.iec.gov.br/bitstreams/1320f9a9-e307-4285-99e2-bce83c3c234d/downloadf08431e96b45f44b4ebdfa0e055e87ebMD55THUMBNAILMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdf.jpgMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdf.jpgGenerated Thumbnailimage/jpeg6077https://patua.iec.gov.br/bitstreams/bf977832-0ffe-4cf8-9e31-6e7143038db8/downloadf95063e9db978da2c3323f4a82565908MD56ORIGINALMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdfMethylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings.pdfapplication/pdf1866832https://patua.iec.gov.br/bitstreams/7fbee89a-1ad3-4efd-b69d-c5489715a113/download85f416e8aeb6d511b248273a43256752MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
title |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
spellingShingle |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings Souza-Rodrigues, Renata Duarte de Polpa Dentária / patologia Compostos de Metilmercúrio / toxicidade Amálgama Dentário / toxicidade Estresse Oxidativo |
title_short |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
title_full |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
title_fullStr |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
title_full_unstemmed |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
title_sort |
Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
author |
Souza-Rodrigues, Renata Duarte de |
author_facet |
Souza-Rodrigues, Renata Duarte de Puty, Bruna Bonfim, Laís Nogueira, Lygia Sega Nascimento, Priscila Cunha Bittencourt, Leonardo Oliveira Couto, Roberta Souza D'Almeida Barboza, Carlos Augusto Galvão Oliveira, Edivaldo Herculano Corrêa de Marques, Marcia Martins Lima, Rafael Rodrigues |
author_role |
author |
author2 |
Puty, Bruna Bonfim, Laís Nogueira, Lygia Sega Nascimento, Priscila Cunha Bittencourt, Leonardo Oliveira Couto, Roberta Souza D'Almeida Barboza, Carlos Augusto Galvão Oliveira, Edivaldo Herculano Corrêa de Marques, Marcia Martins Lima, Rafael Rodrigues |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza-Rodrigues, Renata Duarte de Puty, Bruna Bonfim, Laís Nogueira, Lygia Sega Nascimento, Priscila Cunha Bittencourt, Leonardo Oliveira Couto, Roberta Souza D'Almeida Barboza, Carlos Augusto Galvão Oliveira, Edivaldo Herculano Corrêa de Marques, Marcia Martins Lima, Rafael Rodrigues |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Polpa Dentária / patologia Compostos de Metilmercúrio / toxicidade Amálgama Dentário / toxicidade Estresse Oxidativo |
topic |
Polpa Dentária / patologia Compostos de Metilmercúrio / toxicidade Amálgama Dentário / toxicidade Estresse Oxidativo |
description |
Background: Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods: Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 μM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 μM MeHg were tested by measuring glutathione (GSH) level. Results: It was observed that cell viability decreased significantly after exposure to 2.5 and 5 μM of MeHg, but the metabolic viability only decreased significantly at 5 μM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 μM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-06-21T14:33:59Z |
dc.date.available.fl_str_mv |
2021-06-21T14:33:59Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA-RODRIGUES, Renata Duarte de et al. Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ, v. 9, n. 11114, June 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4329 |
dc.identifier.issn.-.fl_str_mv |
2167-8359 |
dc.identifier.doi.-.fl_str_mv |
10.7717/peerj.11114 |
identifier_str_mv |
SOUZA-RODRIGUES, Renata Duarte de et al. Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings. PeerJ, v. 9, n. 11114, June 2021. 2167-8359 10.7717/peerj.11114 |
url |
https://patua.iec.gov.br/handle/iec/4329 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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PeerJ |
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PeerJ |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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