Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3128 |
Resumo: | The Human T-cell Lymphotropic Virus (HTLV-1) is a Deltaretrovírus that was first isolated in the 1970s, and associated with Adult T-cell Leucemia-Lymphoma (ATLL), and subsequently to Tropical Spastic Paraparesis-Myelopathy (TSP/HAM). The genetic diversity of the virus varies among geographic regions, although its mutation rate is very low (approximately 1% per thousand years) in comparison with other viruses. The present study determined the genetic diversity of HTLV-1 in the metropolitan region of Belém, in northern Brazil. Blood samples were obtained from patients at the UFPA Tropical Medicine Nucleus between January 2010 and December 2013. The DNA was extracted and the PX region of the HTLV was amplified using nested PCR. The positive samples were then digested using the Taq1 enzyme for the identification and differentiation of the HTLV-1 and HTLV-2. The 5’LTR region of the positive HTLV-1 samples were amplified by nested PCR, and then sequenced genetically. The phylogenetic analysis of the samples was based on the maximum likelihood method and the evolutionary profile was analyzed by the Bayesian approach. Overall, 78 samples tested positive for HTLV-1, and 44 were analyzed here. The aA (cosmopolitan-transcontinental) subtype was recorded in all the samples. The following evolutionary rates were recorded for the different subtypes–a: 2.10−3, b: 2.69. 10−2, c: 6.23. 10−2, d: 3.08. 10−2, e: 6. 10−2, f: 1.78. 10−3, g: 2.2. 10−2 mutations per site per year. The positive HTLV-1 samples tested in the present study were characterized by their low genetic diversity and high degree of stability. |
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Nobre, Akim Felipe SantosAlmeida, Danilo de SouzaFerreira, Louise CantoFerreira, Deimy LimaSousa Júnior, Edivaldo CostaViana, Maria de Nazaré do Socorro de AlmeidaSilva, Ingrid ChristianePinheiro, Bruna TelesFerrari, Stephen FrancisLinhares, Alexandre da CostaIshikawa, Edna Aoba YassuiSousa, Rita Catarina MedeirosSousa, Maisa Silvia de2018-04-09T18:17:21Z2018-04-09T18:17:21Z2018NOBRE, Akim Felipe Santos et al. Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin. PLoS One, v. 13, n. 3, p. e0194184, Mar. 2018. DOI: https://doi.org/10.1371/journal.pone.0194184. Disponível em: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0194184&type=printable.1932-6203https://patua.iec.gov.br/handle/iec/312810.1371/journal.pone.0194184The Human T-cell Lymphotropic Virus (HTLV-1) is a Deltaretrovírus that was first isolated in the 1970s, and associated with Adult T-cell Leucemia-Lymphoma (ATLL), and subsequently to Tropical Spastic Paraparesis-Myelopathy (TSP/HAM). The genetic diversity of the virus varies among geographic regions, although its mutation rate is very low (approximately 1% per thousand years) in comparison with other viruses. The present study determined the genetic diversity of HTLV-1 in the metropolitan region of Belém, in northern Brazil. Blood samples were obtained from patients at the UFPA Tropical Medicine Nucleus between January 2010 and December 2013. The DNA was extracted and the PX region of the HTLV was amplified using nested PCR. The positive samples were then digested using the Taq1 enzyme for the identification and differentiation of the HTLV-1 and HTLV-2. The 5’LTR region of the positive HTLV-1 samples were amplified by nested PCR, and then sequenced genetically. The phylogenetic analysis of the samples was based on the maximum likelihood method and the evolutionary profile was analyzed by the Bayesian approach. Overall, 78 samples tested positive for HTLV-1, and 44 were analyzed here. The aA (cosmopolitan-transcontinental) subtype was recorded in all the samples. The following evolutionary rates were recorded for the different subtypes–a: 2.10−3, b: 2.69. 10−2, c: 6.23. 10−2, d: 3.08. 10−2, e: 6. 10−2, f: 1.78. 10−3, g: 2.2. 10−2 mutations per site per year. The positive HTLV-1 samples tested in the present study were characterized by their low genetic diversity and high degree of stability.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal de Sergipe. Sergipe, SE, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.engPublic Library of ScienceLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírus 1 Linfotrópico T Humano / isolamento & purificaçãoVariação Genética / genéticaDeltaretrovirusRegião Norte (BR)Belém (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdfLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdfapplication/pdf9528217https://patua.iec.gov.br/bitstreams/c997a5b1-2085-49cc-b27b-d5a618531d83/downloaddedd6980cbf31e9686064bab8535015fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/d6a54d96-d5ef-4c0c-af44-5a0e4551c9f5/download52f1732ea66fbd1123abe39f5373b797MD52TEXTLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdf.txtLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdf.txtExtracted texttext/plain33612https://patua.iec.gov.br/bitstreams/69f4aae4-3301-40a1-bc4c-be7626c6f182/download32bbc0833d60b6a405d275fd99c33cfcMD55THUMBNAILLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdf.jpgLow genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin.pdf.jpgGenerated Thumbnailimage/jpeg5923https://patua.iec.gov.br/bitstreams/16b9c6a2-424b-4026-b1d6-03530b84b483/download1c2280a1afedf89efed0c6c38af3598fMD56iec/31282023-04-03 14:08:25.35oai:patua.iec.gov.br:iec/3128https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-04-03T14:08:25Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
title |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
spellingShingle |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin Nobre, Akim Felipe Santos Vírus 1 Linfotrópico T Humano / isolamento & purificação Variação Genética / genética Deltaretrovirus Região Norte (BR) Belém (PA) |
title_short |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
title_full |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
title_fullStr |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
title_full_unstemmed |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
title_sort |
Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin |
author |
Nobre, Akim Felipe Santos |
author_facet |
Nobre, Akim Felipe Santos Almeida, Danilo de Souza Ferreira, Louise Canto Ferreira, Deimy Lima Sousa Júnior, Edivaldo Costa Viana, Maria de Nazaré do Socorro de Almeida Silva, Ingrid Christiane Pinheiro, Bruna Teles Ferrari, Stephen Francis Linhares, Alexandre da Costa Ishikawa, Edna Aoba Yassui Sousa, Rita Catarina Medeiros Sousa, Maisa Silvia de |
author_role |
author |
author2 |
Almeida, Danilo de Souza Ferreira, Louise Canto Ferreira, Deimy Lima Sousa Júnior, Edivaldo Costa Viana, Maria de Nazaré do Socorro de Almeida Silva, Ingrid Christiane Pinheiro, Bruna Teles Ferrari, Stephen Francis Linhares, Alexandre da Costa Ishikawa, Edna Aoba Yassui Sousa, Rita Catarina Medeiros Sousa, Maisa Silvia de |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nobre, Akim Felipe Santos Almeida, Danilo de Souza Ferreira, Louise Canto Ferreira, Deimy Lima Sousa Júnior, Edivaldo Costa Viana, Maria de Nazaré do Socorro de Almeida Silva, Ingrid Christiane Pinheiro, Bruna Teles Ferrari, Stephen Francis Linhares, Alexandre da Costa Ishikawa, Edna Aoba Yassui Sousa, Rita Catarina Medeiros Sousa, Maisa Silvia de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Vírus 1 Linfotrópico T Humano / isolamento & purificação Variação Genética / genética Deltaretrovirus Região Norte (BR) Belém (PA) |
topic |
Vírus 1 Linfotrópico T Humano / isolamento & purificação Variação Genética / genética Deltaretrovirus Região Norte (BR) Belém (PA) |
description |
The Human T-cell Lymphotropic Virus (HTLV-1) is a Deltaretrovírus that was first isolated in the 1970s, and associated with Adult T-cell Leucemia-Lymphoma (ATLL), and subsequently to Tropical Spastic Paraparesis-Myelopathy (TSP/HAM). The genetic diversity of the virus varies among geographic regions, although its mutation rate is very low (approximately 1% per thousand years) in comparison with other viruses. The present study determined the genetic diversity of HTLV-1 in the metropolitan region of Belém, in northern Brazil. Blood samples were obtained from patients at the UFPA Tropical Medicine Nucleus between January 2010 and December 2013. The DNA was extracted and the PX region of the HTLV was amplified using nested PCR. The positive samples were then digested using the Taq1 enzyme for the identification and differentiation of the HTLV-1 and HTLV-2. The 5’LTR region of the positive HTLV-1 samples were amplified by nested PCR, and then sequenced genetically. The phylogenetic analysis of the samples was based on the maximum likelihood method and the evolutionary profile was analyzed by the Bayesian approach. Overall, 78 samples tested positive for HTLV-1, and 44 were analyzed here. The aA (cosmopolitan-transcontinental) subtype was recorded in all the samples. The following evolutionary rates were recorded for the different subtypes–a: 2.10−3, b: 2.69. 10−2, c: 6.23. 10−2, d: 3.08. 10−2, e: 6. 10−2, f: 1.78. 10−3, g: 2.2. 10−2 mutations per site per year. The positive HTLV-1 samples tested in the present study were characterized by their low genetic diversity and high degree of stability. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-09T18:17:21Z |
dc.date.available.fl_str_mv |
2018-04-09T18:17:21Z |
dc.date.issued.fl_str_mv |
2018 |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOBRE, Akim Felipe Santos et al. Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin. PLoS One, v. 13, n. 3, p. e0194184, Mar. 2018. DOI: https://doi.org/10.1371/journal.pone.0194184. Disponível em: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0194184&type=printable. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3128 |
dc.identifier.issn.-.fl_str_mv |
1932-6203 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pone.0194184 |
identifier_str_mv |
NOBRE, Akim Felipe Santos et al. Low genetic diversity of the Human T-cell Lymphotropic Virus (HTLV-1) in an endemic area of the Brazilian Amazon basin. PLoS One, v. 13, n. 3, p. e0194184, Mar. 2018. DOI: https://doi.org/10.1371/journal.pone.0194184. Disponível em: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0194184&type=printable. 1932-6203 10.1371/journal.pone.0194184 |
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https://patua.iec.gov.br/handle/iec/3128 |
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Public Library of Science |
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Public Library of Science |
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