Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Riccio, Evelyn Kety Pratt
Data de Publicação: 2005
Outros Autores: Zalis, Mariano Gustavo, Guedes, Helena Cristina Balthazar, Banic, Dalma Maria, Souza, José Maria de, Alecrim, Wilson, Camus, Daniel, Daniel-Ribeiro, Cláudio Tadeu, Cruz, Maria de Fátima Ferreira da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3576
Resumo: In this work we investigated the frequency of polymorphism in exon II of the gene encoding most of the amino-terminal region of the serine rich antigen (SERA) in Plasmodium falciparum field samples. The blood samples were colleted from P. falciparum infected individuals in three areas of the Brazilian Amazon. Two fragments have been characterized by polymerase chain reaction: one of 175 bp corresponding to the repeat region with 5 octamer units and one other of 199 bp related to the 6 repeat octamer units of SERA protein. The 199 bp fragment was the predominant one in all the studied areas. The higher frequency of this fragment has not been described before and could be explained by an immunological selection of the plasmodial population in the infected individuals under study. Since repeat motifs in the amino-terminal region of SERA contain epitopes recognized by parasite-inhibitor antibodies, data reported here suggest that the analysis of the polymorphism of P. falciparum isolates in different geographical areas is a preliminary stage before the final drawing of an universal vaccine against malaria can be reached.
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spelling Riccio, Evelyn Kety PrattZalis, Mariano GustavoGuedes, Helena Cristina BalthazarBanic, Dalma MariaSouza, José Maria deAlecrim, WilsonCamus, DanielDaniel-Ribeiro, Cláudio TadeuCruz, Maria de Fátima Ferreira da2019-01-23T17:12:01Z2019-01-23T17:12:01Z2005RICCIO, Evelyn Kety Pratt et al. Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil. Memórias do Instituto Oswaldo Cruz, v. 100, n. 1, 47-49, Feb. 2005. DOI: https://doi.org/10.1590/S0074-02762005000100009. Disponível em: https://www.scielo.br/j/mioc/a/ZRSxfRHdgjgrgbWNHnL7H6c/?format=pdf&lang=en.0074-0276https://patua.iec.gov.br/handle/iec/357610.1590/S0074-02762005000100009In this work we investigated the frequency of polymorphism in exon II of the gene encoding most of the amino-terminal region of the serine rich antigen (SERA) in Plasmodium falciparum field samples. The blood samples were colleted from P. falciparum infected individuals in three areas of the Brazilian Amazon. Two fragments have been characterized by polymerase chain reaction: one of 175 bp corresponding to the repeat region with 5 octamer units and one other of 199 bp related to the 6 repeat octamer units of SERA protein. The 199 bp fragment was the predominant one in all the studied areas. The higher frequency of this fragment has not been described before and could be explained by an immunological selection of the plasmodial population in the infected individuals under study. Since repeat motifs in the amino-terminal region of SERA contain epitopes recognized by parasite-inhibitor antibodies, data reported here suggest that the analysis of the polymorphism of P. falciparum isolates in different geographical areas is a preliminary stage before the final drawing of an universal vaccine against malaria can be reached.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da OMS para Pesquisa e Treinamento em Imunologia de Doenças Parasitárias. Departamento de Imunologia. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da OMS para Pesquisa e Treinamento em Imunologia de Doenças Parasitárias. Departamento de Imunologia. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Fundação de Medicina Tropical do Amazonas. Centro Universitário Nilton Lins. Manaus, AM, Brasil.Université de Lille. Faculte de Medecine. Laboratoire de Parasitologie-Mycologie. Lille, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da OMS para Pesquisa e Treinamento em Imunologia de Doenças Parasitárias. Departamento de Imunologia. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro Colaborador da OMS para Pesquisa e Treinamento em Imunologia de Doenças Parasitárias. Departamento de Imunologia. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.engInstituto Oswaldo CruzGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlasmodium falciparum / isolamento & purificaçãoPolimorfismo Genético / imunologiaMalária Falciparum / prevenção & controleExonucleasesinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdfGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdfapplication/pdf35558https://patua.iec.gov.br/bitstreams/0edde5a7-6169-485e-90b9-de85623da939/downloadf718ec1f06654849bcf38ff6c2af5416MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/6ef64aa5-b426-4563-9aa0-f52266bc1d6b/download11832eea31b16df8613079d742d61793MD52TEXTGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdf.txtGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdf.txtExtracted texttext/plain16778https://patua.iec.gov.br/bitstreams/9fb1b364-6663-4e3f-9081-b200fbbebba1/download6aaa5abc9c3beb9a028df70413367dcaMD55THUMBNAILGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdf.jpgGenetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil.pdf.jpgGenerated Thumbnailimage/jpeg6216https://patua.iec.gov.br/bitstreams/e4db5e02-ac33-468a-b2e2-19dc242f4a26/download4b8e6bc2e17d95430b473e6085321b94MD56iec/35762022-10-20 21:10:45.336oai:patua.iec.gov.br:iec/3576https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:10:45Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
title Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
spellingShingle Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
Riccio, Evelyn Kety Pratt
Plasmodium falciparum / isolamento & purificação
Polimorfismo Genético / imunologia
Malária Falciparum / prevenção & controle
Exonucleases
title_short Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
title_full Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
title_fullStr Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
title_full_unstemmed Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
title_sort Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil
author Riccio, Evelyn Kety Pratt
author_facet Riccio, Evelyn Kety Pratt
Zalis, Mariano Gustavo
Guedes, Helena Cristina Balthazar
Banic, Dalma Maria
Souza, José Maria de
Alecrim, Wilson
Camus, Daniel
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
author_role author
author2 Zalis, Mariano Gustavo
Guedes, Helena Cristina Balthazar
Banic, Dalma Maria
Souza, José Maria de
Alecrim, Wilson
Camus, Daniel
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Riccio, Evelyn Kety Pratt
Zalis, Mariano Gustavo
Guedes, Helena Cristina Balthazar
Banic, Dalma Maria
Souza, José Maria de
Alecrim, Wilson
Camus, Daniel
Daniel-Ribeiro, Cláudio Tadeu
Cruz, Maria de Fátima Ferreira da
dc.subject.decsPrimary.pt_BR.fl_str_mv Plasmodium falciparum / isolamento & purificação
Polimorfismo Genético / imunologia
Malária Falciparum / prevenção & controle
Exonucleases
topic Plasmodium falciparum / isolamento & purificação
Polimorfismo Genético / imunologia
Malária Falciparum / prevenção & controle
Exonucleases
description In this work we investigated the frequency of polymorphism in exon II of the gene encoding most of the amino-terminal region of the serine rich antigen (SERA) in Plasmodium falciparum field samples. The blood samples were colleted from P. falciparum infected individuals in three areas of the Brazilian Amazon. Two fragments have been characterized by polymerase chain reaction: one of 175 bp corresponding to the repeat region with 5 octamer units and one other of 199 bp related to the 6 repeat octamer units of SERA protein. The 199 bp fragment was the predominant one in all the studied areas. The higher frequency of this fragment has not been described before and could be explained by an immunological selection of the plasmodial population in the infected individuals under study. Since repeat motifs in the amino-terminal region of SERA contain epitopes recognized by parasite-inhibitor antibodies, data reported here suggest that the analysis of the polymorphism of P. falciparum isolates in different geographical areas is a preliminary stage before the final drawing of an universal vaccine against malaria can be reached.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-01-23T17:12:01Z
dc.date.available.fl_str_mv 2019-01-23T17:12:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv RICCIO, Evelyn Kety Pratt et al. Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil. Memórias do Instituto Oswaldo Cruz, v. 100, n. 1, 47-49, Feb. 2005. DOI: https://doi.org/10.1590/S0074-02762005000100009. Disponível em: https://www.scielo.br/j/mioc/a/ZRSxfRHdgjgrgbWNHnL7H6c/?format=pdf&lang=en.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3576
dc.identifier.issn.-.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0074-02762005000100009
identifier_str_mv RICCIO, Evelyn Kety Pratt et al. Genetic polymorphism of the serine rich antigen N-terminal region in Plasmodium falciparum field isolates from Brazil. Memórias do Instituto Oswaldo Cruz, v. 100, n. 1, 47-49, Feb. 2005. DOI: https://doi.org/10.1590/S0074-02762005000100009. Disponível em: https://www.scielo.br/j/mioc/a/ZRSxfRHdgjgrgbWNHnL7H6c/?format=pdf&lang=en.
0074-0276
10.1590/S0074-02762005000100009
url https://patua.iec.gov.br/handle/iec/3576
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instacron:IEC
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institution IEC
reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
collection Repositório Digital do Instituto Evandro Chagas (Patuá)
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