Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)

Detalhes bibliográficos
Autor(a) principal: Furo, Ivanete de Oliveira
Data de Publicação: 2020
Outros Autores: Krestschmer, Rafael, O'Brien, Patricia Caroline, Pereira, Jorge C, Garnero, Analía del Valle, Gunski, Ricardo José, O'Connor, Rebecca E, Griffin, Darren Karl, Gomes, Anderson José Baia, Ferguson-Smith, Malcom Andrew, Oliveira, Edivaldo Herculano Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4141
Resumo: Myiopsitta monachus is a small Neotropical parrot (Psittaciformes: Arini Tribe) from subtropical and temperate regions of South America. It has a diploid chromosome number 2n = 48, different from other members of the Arini Tribe that have usually 70 chromosomes. The species has the lowest 2n within the Arini Tribe. In this study, we combined comparative chromosome painting with probes generated from chromosomes of Gallus gallus and Leucopternis albicollis, and FISH with bacterial artificial chromosomes (BACs) selected from the genome library of G. gallus with the aim to shed light on the dynamics of genome reorganization in M. monachus in the phylogenetic context. The homology maps showed a great number of fissions in macrochromosomes, and many fusions between microchromosomes and fragments of macrochromosomes. Our phylogenetic analysis by Maximum Parsimony agree with molecular data, placing M. monachus in a basal position within the Arini Tribe, together with Amazona aestiva (short tailed species). In M. monachus many chromosome rearrangements were found to represent autopomorphic characters, indicating that after this species split as an independent branch, an intensive karyotype reorganization took place. In addition, our results show that M. monachus probes generated by flow cytometry provide novel cytogenetic tools for the detection of avian chromosome rearrangements, since this species presents breakpoints that have not been described in other species.
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spelling Furo, Ivanete de OliveiraKrestschmer, RafaelO'Brien, Patricia CarolinePereira, Jorge CGarnero, Analía del ValleGunski, Ricardo JoséO'Connor, Rebecca EGriffin, Darren KarlGomes, Anderson José BaiaFerguson-Smith, Malcom AndrewOliveira, Edivaldo Herculano Corrêa de2020-08-03T17:57:45Z2020-08-03T17:57:45Z2020FURO, Ivanete de Oliveira et al. Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae). Frontiers in Genetics, v. 11, n. 721, p. 1-15, July 2020.1664-8021https://patua.iec.gov.br/handle/iec/414110.3389/fgene.2020.00721Myiopsitta monachus is a small Neotropical parrot (Psittaciformes: Arini Tribe) from subtropical and temperate regions of South America. It has a diploid chromosome number 2n = 48, different from other members of the Arini Tribe that have usually 70 chromosomes. The species has the lowest 2n within the Arini Tribe. In this study, we combined comparative chromosome painting with probes generated from chromosomes of Gallus gallus and Leucopternis albicollis, and FISH with bacterial artificial chromosomes (BACs) selected from the genome library of G. gallus with the aim to shed light on the dynamics of genome reorganization in M. monachus in the phylogenetic context. The homology maps showed a great number of fissions in macrochromosomes, and many fusions between microchromosomes and fragments of macrochromosomes. Our phylogenetic analysis by Maximum Parsimony agree with molecular data, placing M. monachus in a basal position within the Arini Tribe, together with Amazona aestiva (short tailed species). In M. monachus many chromosome rearrangements were found to represent autopomorphic characters, indicating that after this species split as an independent branch, an intensive karyotype reorganization took place. In addition, our results show that M. monachus probes generated by flow cytometry provide novel cytogenetic tools for the detection of avian chromosome rearrangements, since this species presents breakpoints that have not been described in other species.This research was partially funded by a grant to EO from CNPq (307382/2019-2) and to MF-S from the Welcome Trust in support of the Cambridge Resource Center for Comparative Genomics, by the Biotechnology and Biological Sciences Research Council (BB/K008161/1) to the University of Kent, and by the project UIDB/CVT/00772/2020 funded by the Fundação para a Ciência e Tecnologia (FCT).Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / University of Cambridge. Cambridge Resource Centre for Comparative Genomics. Department of Veterinary Medicine. Cambridge, United Kingdom.Universidade Federal do Rio Grande do Sul. Departamento de Genética. Porto Alegre, RS, Brazil / University of Kent. School of Biosciences. Canterbury, United Kingdom.University of Cambridge. Cambridge Resource Centre for Comparative Genomics. Department of Veterinary Medicine. Cambridge, United Kingdom.University of Tràs-os-Montes and Alto Douro. Animal and Veterinary Research Centre. Vila Real, Portugal.Universidade Federal do Pampa. Laboratório de Diversidade Genética Animal. São Gabriel, RS, Brazil.University of Kent. School of Biosciences. Canterbury, United Kingdom.University of Kent. School of Biosciences. Canterbury, United Kingdom.Instituto Federal do Pará. Abaetetuba, PA, Brazil.University of Cambridge. Cambridge Resource Centre for Comparative Genomics. Department of Veterinary Medicine. Cambridge, United Kingdom.Universidade Federal do Pará. Instituto de Ciências Biológicas. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brazil.engFrontiers MediaChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePapagaios / anatomia & histologiaPapagaios / classificaçãoColoração Cromossômica / métodosCariótipoRearranjo Gênicoinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdfChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdfapplication/pdf5035973https://patua.iec.gov.br/bitstreams/702b52c7-4c45-4eea-84d3-15975d9da5b3/download03ca0e152a5f2bfb21f7c9a864ace35aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/b4a8097d-72db-4258-a6a3-4aaa15ed8b7d/download11832eea31b16df8613079d742d61793MD52TEXTChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdf.txtChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdf.txtExtracted texttext/plain56920https://patua.iec.gov.br/bitstreams/c54c1393-4b22-4d3f-953e-0ee15b606128/downloadf4ee5b05c5752371bebe4225d229fbe2MD55THUMBNAILChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdf.jpgChromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae).pdf.jpgGenerated Thumbnailimage/jpeg5599https://patua.iec.gov.br/bitstreams/70081d7c-df53-4cba-b3b1-41177d7e4ebf/downloada080cb1400b519512653bff86a99d96fMD56iec/41412022-10-21 00:34:20.381oai:patua.iec.gov.br:iec/4141https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:34:20Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
title Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
spellingShingle Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
Furo, Ivanete de Oliveira
Papagaios / anatomia & histologia
Papagaios / classificação
Coloração Cromossômica / métodos
Cariótipo
Rearranjo Gênico
title_short Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
title_full Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
title_fullStr Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
title_full_unstemmed Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
title_sort Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae)
author Furo, Ivanete de Oliveira
author_facet Furo, Ivanete de Oliveira
Krestschmer, Rafael
O'Brien, Patricia Caroline
Pereira, Jorge C
Garnero, Analía del Valle
Gunski, Ricardo José
O'Connor, Rebecca E
Griffin, Darren Karl
Gomes, Anderson José Baia
Ferguson-Smith, Malcom Andrew
Oliveira, Edivaldo Herculano Corrêa de
author_role author
author2 Krestschmer, Rafael
O'Brien, Patricia Caroline
Pereira, Jorge C
Garnero, Analía del Valle
Gunski, Ricardo José
O'Connor, Rebecca E
Griffin, Darren Karl
Gomes, Anderson José Baia
Ferguson-Smith, Malcom Andrew
Oliveira, Edivaldo Herculano Corrêa de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Furo, Ivanete de Oliveira
Krestschmer, Rafael
O'Brien, Patricia Caroline
Pereira, Jorge C
Garnero, Analía del Valle
Gunski, Ricardo José
O'Connor, Rebecca E
Griffin, Darren Karl
Gomes, Anderson José Baia
Ferguson-Smith, Malcom Andrew
Oliveira, Edivaldo Herculano Corrêa de
dc.subject.decsPrimary.pt_BR.fl_str_mv Papagaios / anatomia & histologia
Papagaios / classificação
Coloração Cromossômica / métodos
Cariótipo
Rearranjo Gênico
topic Papagaios / anatomia & histologia
Papagaios / classificação
Coloração Cromossômica / métodos
Cariótipo
Rearranjo Gênico
description Myiopsitta monachus is a small Neotropical parrot (Psittaciformes: Arini Tribe) from subtropical and temperate regions of South America. It has a diploid chromosome number 2n = 48, different from other members of the Arini Tribe that have usually 70 chromosomes. The species has the lowest 2n within the Arini Tribe. In this study, we combined comparative chromosome painting with probes generated from chromosomes of Gallus gallus and Leucopternis albicollis, and FISH with bacterial artificial chromosomes (BACs) selected from the genome library of G. gallus with the aim to shed light on the dynamics of genome reorganization in M. monachus in the phylogenetic context. The homology maps showed a great number of fissions in macrochromosomes, and many fusions between microchromosomes and fragments of macrochromosomes. Our phylogenetic analysis by Maximum Parsimony agree with molecular data, placing M. monachus in a basal position within the Arini Tribe, together with Amazona aestiva (short tailed species). In M. monachus many chromosome rearrangements were found to represent autopomorphic characters, indicating that after this species split as an independent branch, an intensive karyotype reorganization took place. In addition, our results show that M. monachus probes generated by flow cytometry provide novel cytogenetic tools for the detection of avian chromosome rearrangements, since this species presents breakpoints that have not been described in other species.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-03T17:57:45Z
dc.date.available.fl_str_mv 2020-08-03T17:57:45Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FURO, Ivanete de Oliveira et al. Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae). Frontiers in Genetics, v. 11, n. 721, p. 1-15, July 2020.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4141
dc.identifier.issn.-.fl_str_mv 1664-8021
dc.identifier.doi.-.fl_str_mv 10.3389/fgene.2020.00721
identifier_str_mv FURO, Ivanete de Oliveira et al. Chromosomal evolution in the phylogenetic context: a remarkable karyotype reorganization in neotropical Parrot Myiopsitta monachus (Psittacidae). Frontiers in Genetics, v. 11, n. 721, p. 1-15, July 2020.
1664-8021
10.3389/fgene.2020.00721
url https://patua.iec.gov.br/handle/iec/4141
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