The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3548 |
Resumo: | Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease. |
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Silva, Luciana MotaSousa, Jorge Rodrigues deHirai, Kelly EmiDias Junior, Leônidas BragaFurlaneto, Ismari PeriniCarneiro, Francisca Regina OliveiraAarão, Tinara Leila de SouzaSotto, Mirian NacagamiQuaresma, Juarez Antônio Simões2018-12-21T18:44:10Z2018-12-21T18:44:10Z2018SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf.1178-6973https://patua.iec.gov.br/handle/iec/354810.2147/IDR.S172806Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease.CNPq/Brazil (grants numbers 302553/2015-0 and 116427/2016-7). Program of support for qualified production—PAPQ/UFPA.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.Federal Do Para University. Tropical Medicine Center. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.University, São Paulo. School of Medicine. São Paulo, SP, Brazil / Sao Paulo University. Tropical Medicine Institute. São Paulo, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil / Federal Do Para University. Tropical Medicine Center. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Sao Paulo University. Tropical Medicine Institute. São Paulo, Brazil.engDove Medical PressThe inflammasome in leprosy skin lesions: an immunohistochemical evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHanseníaseMycobacterium leprae / patogenicidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdfThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdfapplication/pdf5177980https://patua.iec.gov.br/bitstreams/a346127c-d181-4302-905a-d874cbc79e6a/downloadd0c5013f9777605bcc8ab1f4621137a3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/6884215c-ad31-4e1b-8b8e-bc50db19e065/download52f1732ea66fbd1123abe39f5373b797MD52TEXTThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.txtThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.txtExtracted texttext/plain47490https://patua.iec.gov.br/bitstreams/0c85f9a7-24b8-4865-8065-86387da296f7/download03fc56e3f5b1d42d8b4db99a1eeb4517MD55THUMBNAILThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.jpgThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.jpgGenerated Thumbnailimage/jpeg5679https://patua.iec.gov.br/bitstreams/f6071eac-d0bc-4528-b57d-c76d1a33d00e/download4aaed7efaf6632b61d7073bf702c609fMD56iec/35482022-10-20 21:36:57.305oai:patua.iec.gov.br:iec/3548https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:36:57Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
title |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
spellingShingle |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation Silva, Luciana Mota Hanseníase Mycobacterium leprae / patogenicidade |
title_short |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
title_full |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
title_fullStr |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
title_full_unstemmed |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
title_sort |
The inflammasome in leprosy skin lesions: an immunohistochemical evaluation |
author |
Silva, Luciana Mota |
author_facet |
Silva, Luciana Mota Sousa, Jorge Rodrigues de Hirai, Kelly Emi Dias Junior, Leônidas Braga Furlaneto, Ismari Perini Carneiro, Francisca Regina Oliveira Aarão, Tinara Leila de Souza Sotto, Mirian Nacagami Quaresma, Juarez Antônio Simões |
author_role |
author |
author2 |
Sousa, Jorge Rodrigues de Hirai, Kelly Emi Dias Junior, Leônidas Braga Furlaneto, Ismari Perini Carneiro, Francisca Regina Oliveira Aarão, Tinara Leila de Souza Sotto, Mirian Nacagami Quaresma, Juarez Antônio Simões |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Luciana Mota Sousa, Jorge Rodrigues de Hirai, Kelly Emi Dias Junior, Leônidas Braga Furlaneto, Ismari Perini Carneiro, Francisca Regina Oliveira Aarão, Tinara Leila de Souza Sotto, Mirian Nacagami Quaresma, Juarez Antônio Simões |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Hanseníase Mycobacterium leprae / patogenicidade |
topic |
Hanseníase Mycobacterium leprae / patogenicidade |
description |
Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-12-21T18:44:10Z |
dc.date.available.fl_str_mv |
2018-12-21T18:44:10Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3548 |
dc.identifier.issn.-.fl_str_mv |
1178-6973 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.2147/IDR.S172806 |
identifier_str_mv |
SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf. 1178-6973 10.2147/IDR.S172806 |
url |
https://patua.iec.gov.br/handle/iec/3548 |
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eng |
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eng |
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Dove Medical Press |
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Dove Medical Press |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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