The inflammasome in leprosy skin lesions: an immunohistochemical evaluation

Detalhes bibliográficos
Autor(a) principal: Silva, Luciana Mota
Data de Publicação: 2018
Outros Autores: Sousa, Jorge Rodrigues de, Hirai, Kelly Emi, Dias Junior, Leônidas Braga, Furlaneto, Ismari Perini, Carneiro, Francisca Regina Oliveira, Aarão, Tinara Leila de Souza, Sotto, Mirian Nacagami, Quaresma, Juarez Antônio Simões
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3548
Resumo: Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease.
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spelling Silva, Luciana MotaSousa, Jorge Rodrigues deHirai, Kelly EmiDias Junior, Leônidas BragaFurlaneto, Ismari PeriniCarneiro, Francisca Regina OliveiraAarão, Tinara Leila de SouzaSotto, Mirian NacagamiQuaresma, Juarez Antônio Simões2018-12-21T18:44:10Z2018-12-21T18:44:10Z2018SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf.1178-6973https://patua.iec.gov.br/handle/iec/354810.2147/IDR.S172806Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease.CNPq/Brazil (grants numbers 302553/2015-0 and 116427/2016-7). Program of support for qualified production—PAPQ/UFPA.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.Federal Do Para University. Tropical Medicine Center. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil.University, São Paulo. School of Medicine. São Paulo, SP, Brazil / Sao Paulo University. Tropical Medicine Institute. São Paulo, Brazil.State University of Para. Center of Biological and Health Science. Belem, PA, Brazil / Federal Do Para University. Tropical Medicine Center. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Sao Paulo University. Tropical Medicine Institute. São Paulo, Brazil.engDove Medical PressThe inflammasome in leprosy skin lesions: an immunohistochemical evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHanseníaseMycobacterium leprae / patogenicidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdfThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdfapplication/pdf5177980https://patua.iec.gov.br/bitstreams/a346127c-d181-4302-905a-d874cbc79e6a/downloadd0c5013f9777605bcc8ab1f4621137a3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/6884215c-ad31-4e1b-8b8e-bc50db19e065/download52f1732ea66fbd1123abe39f5373b797MD52TEXTThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.txtThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.txtExtracted texttext/plain47490https://patua.iec.gov.br/bitstreams/0c85f9a7-24b8-4865-8065-86387da296f7/download03fc56e3f5b1d42d8b4db99a1eeb4517MD55THUMBNAILThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.jpgThe inflammasome in leprosy skin lesions: an immunohistochemical evaluation.pdf.jpgGenerated Thumbnailimage/jpeg5679https://patua.iec.gov.br/bitstreams/f6071eac-d0bc-4528-b57d-c76d1a33d00e/download4aaed7efaf6632b61d7073bf702c609fMD56iec/35482022-10-20 21:36:57.305oai:patua.iec.gov.br:iec/3548https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:36:57Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
spellingShingle The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
Silva, Luciana Mota
Hanseníase
Mycobacterium leprae / patogenicidade
title_short The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_full The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_fullStr The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_full_unstemmed The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
title_sort The inflammasome in leprosy skin lesions: an immunohistochemical evaluation
author Silva, Luciana Mota
author_facet Silva, Luciana Mota
Sousa, Jorge Rodrigues de
Hirai, Kelly Emi
Dias Junior, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
Aarão, Tinara Leila de Souza
Sotto, Mirian Nacagami
Quaresma, Juarez Antônio Simões
author_role author
author2 Sousa, Jorge Rodrigues de
Hirai, Kelly Emi
Dias Junior, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
Aarão, Tinara Leila de Souza
Sotto, Mirian Nacagami
Quaresma, Juarez Antônio Simões
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Luciana Mota
Sousa, Jorge Rodrigues de
Hirai, Kelly Emi
Dias Junior, Leônidas Braga
Furlaneto, Ismari Perini
Carneiro, Francisca Regina Oliveira
Aarão, Tinara Leila de Souza
Sotto, Mirian Nacagami
Quaresma, Juarez Antônio Simões
dc.subject.decsPrimary.pt_BR.fl_str_mv Hanseníase
Mycobacterium leprae / patogenicidade
topic Hanseníase
Mycobacterium leprae / patogenicidade
description Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis. Patients and methods: We evaluated the expression of inflammasome markers (nucleotidebinding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotidebinding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Results: The evaluated markers were most upregulated in LL lesions, followed by lesions of TT leprosy and I leprosy. Differences were statistically significant between the I leprosy and LL leprosy forms and between the I leprosy and TT leprosy forms. Positive and significant correlations were found between IL-18 and caspase 1 in LL (r=0.7516, P=0.0012) and TT leprosy (r=0.7366, P=0.0017). In I leprosy, correlations were detected between caspase 1 and IL-1β (r=0.6412, P=0.0182), NLRP1 and IL-18 (r=0.5585, P=0.473), NLRP3 and IL-18 (r=0.6873, P=0.0094), and NLRP1 and NLRP3 (r=0.8040, P=0.0009). Conclusion: The expression of inflammasome markers in LL lesions indicates the ineffectiveness of this protein complex in controlling the infection. Caspase 1 may be involved in the pyroptotic cell death in the lepromatous form of the disease. Inflammasomes may act together in the initial phase of I leprosy; this phenomenon may influence the clinical outcome of the disease.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-21T18:44:10Z
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dc.identifier.citation.fl_str_mv SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf.
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identifier_str_mv SILVA, Luciana Mota et al. The inflammasome in leprosy skin lesions: an immunohistochemical evaluation. Infection and Drug Resistance, v. 11, p. 2231-2240, 2018. DOI: https://doi.org/10.2147/IDR.S172806. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237140/pdf/idr-11-2231.pdf.
1178-6973
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