Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4316 |
Resumo: | The Saffron finch (Sicalis flaveola), a semi-domestic species, is tolerant of human proximity and nesting in roof spaces. Considering the importance of cytogenomic approaches in revealing different aspects of genomic organization and evolution, we provide detailed cytogenetic data for S. flaveola, including the standard Giemsa karyotype, C- and G-banding, repetitive DNA mapping, and bacterial artificial chromosome (BAC) FISH. We also compared our results with the sister groups, Passeriformes and Psittaciformes, bringing new insights into the chromosome and genome evolution of birds. The results revealed contrasting rates of intrachromosomal changes, highlighting the role of SSR (simple short repetition probes) accumulation in the karyotype reorganization. The SSRs showed scattered hybridization, but brighter signals were observed in the microchromosomes and the short arms of Z chromosome in S. flaveola. BACs probes showed conservation of ancestral syntenies of macrochromosomes (except GGA1), as well as the tested microchromosomes. The comparison of our results with previous studies indicates that the great biological diversity observed in Passeriformes was not likely accompanied by interchromosomal changes. In addition, although repetitive sequences often act as hotspots of genome rearrangements, Passeriformes species showed a higher number of signals when compared with the sister group Psittaciformes, indicating that these sequences were not involved in the extensive karyotype reorganization seen in the latter. |
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Krestschmer, RafaelRodrigues, Benilson SilvaBarcellos, Suziane AlvesCosta, Alice LemosCioffi, Marcelo de BelloGarnero, Analía del ValleGunski, Ricardo JoséOliveira, Edivaldo Herculano Corrêa deGriffin, Darren Karl2021-05-31T20:26:34Z2021-05-31T20:26:34Z2021KRESTCHMER Rafael et al. Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves). Animals, v. 11, n. 1456, 2021.2076-2615https://patua.iec.gov.br/handle/iec/431610.3390/ani11051456The Saffron finch (Sicalis flaveola), a semi-domestic species, is tolerant of human proximity and nesting in roof spaces. Considering the importance of cytogenomic approaches in revealing different aspects of genomic organization and evolution, we provide detailed cytogenetic data for S. flaveola, including the standard Giemsa karyotype, C- and G-banding, repetitive DNA mapping, and bacterial artificial chromosome (BAC) FISH. We also compared our results with the sister groups, Passeriformes and Psittaciformes, bringing new insights into the chromosome and genome evolution of birds. The results revealed contrasting rates of intrachromosomal changes, highlighting the role of SSR (simple short repetition probes) accumulation in the karyotype reorganization. The SSRs showed scattered hybridization, but brighter signals were observed in the microchromosomes and the short arms of Z chromosome in S. flaveola. BACs probes showed conservation of ancestral syntenies of macrochromosomes (except GGA1), as well as the tested microchromosomes. The comparison of our results with previous studies indicates that the great biological diversity observed in Passeriformes was not likely accompanied by interchromosomal changes. In addition, although repetitive sequences often act as hotspots of genome rearrangements, Passeriformes species showed a higher number of signals when compared with the sister group Psittaciformes, indicating that these sequences were not involved in the extensive karyotype reorganization seen in the latter.This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. PDE 204792/2018-5, PDJ 151056/2020-0, and PQ 307382/2019-2), and the Biotechnology and Biological Sciences Research Council UK (BB/K008226/1)University of Kent. School of Biosciences. Canterbury, UK.Instituto Federal do Pará. Abaetetuba, PA, Brazil.Universidade Federal do Pampa. Laboratório de Diversidade Genética Animal. São Gabriel, RS, Brazil.Universidade Federal do Pampa. Laboratório de Diversidade Genética Animal. São Gabriel, RS, Brazil.Universidade Federal de São Carlos. Departamento de Genética e Evolução. Centro de Ciências Biológicas e da Saúde. Laboratório de Citogenética de Peixes. São Carlos , SP, Brasil.1Universidade Federal do Pampa. Laboratório de Diversidade Genética Animal. São Gabriel, RS, Brazil.Universidade Federal do Pampa. Laboratório de Diversidade Genética Animal. São Gabriel, RS, Brazil.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.University of Kent. School of Biosciences. Canterbury, UK.engMDPIKaryotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAves / anatomia & histologiaTentilhões / anatomia & histologiaPsittaciformes / anatomia & histologiaRearranjo Gênico / genéticaAnálise Citogenética / métodosCariótipoColoração Cromossômica / genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALKaryotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves).pdfKaryotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves).pdfapplication/pdf2643156https://patua.iec.gov.br/bitstreams/2b14d5e4-9a72-443e-8036-6988fcfb2b5b/download9020e01c2ae56d1ee8cc4aab65e1d74dMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
title |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
spellingShingle |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) Krestschmer, Rafael Aves / anatomia & histologia Tentilhões / anatomia & histologia Psittaciformes / anatomia & histologia Rearranjo Gênico / genética Análise Citogenética / métodos Cariótipo Coloração Cromossômica / genética |
title_short |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
title_full |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
title_fullStr |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
title_full_unstemmed |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
title_sort |
Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves) |
author |
Krestschmer, Rafael |
author_facet |
Krestschmer, Rafael Rodrigues, Benilson Silva Barcellos, Suziane Alves Costa, Alice Lemos Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José Oliveira, Edivaldo Herculano Corrêa de Griffin, Darren Karl |
author_role |
author |
author2 |
Rodrigues, Benilson Silva Barcellos, Suziane Alves Costa, Alice Lemos Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José Oliveira, Edivaldo Herculano Corrêa de Griffin, Darren Karl |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Krestschmer, Rafael Rodrigues, Benilson Silva Barcellos, Suziane Alves Costa, Alice Lemos Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José Oliveira, Edivaldo Herculano Corrêa de Griffin, Darren Karl |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Aves / anatomia & histologia Tentilhões / anatomia & histologia Psittaciformes / anatomia & histologia Rearranjo Gênico / genética Análise Citogenética / métodos Cariótipo |
topic |
Aves / anatomia & histologia Tentilhões / anatomia & histologia Psittaciformes / anatomia & histologia Rearranjo Gênico / genética Análise Citogenética / métodos Cariótipo Coloração Cromossômica / genética |
dc.subject.decsPrimary.pt-BR.fl_str_mv |
Coloração Cromossômica / genética |
description |
The Saffron finch (Sicalis flaveola), a semi-domestic species, is tolerant of human proximity and nesting in roof spaces. Considering the importance of cytogenomic approaches in revealing different aspects of genomic organization and evolution, we provide detailed cytogenetic data for S. flaveola, including the standard Giemsa karyotype, C- and G-banding, repetitive DNA mapping, and bacterial artificial chromosome (BAC) FISH. We also compared our results with the sister groups, Passeriformes and Psittaciformes, bringing new insights into the chromosome and genome evolution of birds. The results revealed contrasting rates of intrachromosomal changes, highlighting the role of SSR (simple short repetition probes) accumulation in the karyotype reorganization. The SSRs showed scattered hybridization, but brighter signals were observed in the microchromosomes and the short arms of Z chromosome in S. flaveola. BACs probes showed conservation of ancestral syntenies of macrochromosomes (except GGA1), as well as the tested microchromosomes. The comparison of our results with previous studies indicates that the great biological diversity observed in Passeriformes was not likely accompanied by interchromosomal changes. In addition, although repetitive sequences often act as hotspots of genome rearrangements, Passeriformes species showed a higher number of signals when compared with the sister group Psittaciformes, indicating that these sequences were not involved in the extensive karyotype reorganization seen in the latter. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-31T20:26:34Z |
dc.date.available.fl_str_mv |
2021-05-31T20:26:34Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
KRESTCHMER Rafael et al. Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves). Animals, v. 11, n. 1456, 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4316 |
dc.identifier.issn.-.fl_str_mv |
2076-2615 |
dc.identifier.doi.-.fl_str_mv |
10.3390/ani11051456 |
identifier_str_mv |
KRESTCHMER Rafael et al. Karyotype evolution and genomic organization of repetitive dnas in the saffron finch, Sicalis flaveola (Passeriformes, aves). Animals, v. 11, n. 1456, 2021. 2076-2615 10.3390/ani11051456 |
url |
https://patua.iec.gov.br/handle/iec/4316 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Instituto Evandro Chagas (IEC) |
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IEC |
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IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
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