Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana

Detalhes bibliográficos
Autor(a) principal: Gomes, Margarete do Socorro Mendonça
Data de Publicação: 2016
Outros Autores: Vieira, José Luiz Fernandes, Cassiano, Gustavo Capatti, Musset, Lise, Legrand, Eric, Nacher, Mathieu, Couto, Vanja Suely Calvosa D’Almeida, Machado, Ricardo Luiz Dantas, Couto, Álvaro Augusto Ribeiro D’Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2511
Resumo: Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana.
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spelling Gomes, Margarete do Socorro MendonçaVieira, José Luiz FernandesCassiano, Gustavo CapattiMusset, LiseLegrand, EricNacher, MathieuCouto, Vanja Suely Calvosa D’AlmeidaMachado, Ricardo Luiz DantasCouto, Álvaro Augusto Ribeiro D’Almeida2017-02-14T11:06:19Z2017-02-14T11:06:19Z201620170214GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016.1678-9946https://patua.iec.gov.br/handle/iec/251110.1590/S1678-9946201658072Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana.The work reported in this manuscript was funded by the National Scientific and Technological Development in the Research Program for Health System Unique (CNPq PPSUS Public Notice No. 00077/2008/ MCT/CNPq/SETEC, Process No. 35.000.162/2009), and Government of the State of Amapá, and also, Central Public Health Laboratory (LACEN) of AmapáSecretaria de Estado da Saúde do Amapá. Laboratório Central. Macapá, AP, Brazil / Universidade Federal do Pará. Belém, PA, Brazil.Universidade Federal do Pará. Belém, PA, Brazil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, SP, Brazil.Secretaria de Estado da Saúde do Amapá. Macapá, AP, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Pará. Belém, PA, Brazil / Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, SP, Brazil.Universidade Federal do Pará. Belém, PA, Brazil.Institut Pasteur de la Guyane. Centre National de Référence du Paludisme. Laboratoire de Parasitologie. Cayenne, French Guiana.Institut Pasteur de la Guyane. Centre National de Référence du Paludisme. Laboratoire de Parasitologie. Cayenne, French Guiana.Université des Antilles et de la Guyane. Cayenne, French Guiana.engUniversidade de São PauloEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guianainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária / transmissãoPlasmodium vivaxMapeamento CromossômicoOiapoque (AP)Brasil (BR)Guiana Francesa (FR)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgGenerated Thumbnailimage/jpeg1587https://patua.iec.gov.br/bitstreams/43af4ce9-433d-494c-bc8f-d4ec290449fa/downloadfadac5c0aa7a0472ea5cfb10266abbdcMD55Evaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgGenerated Thumbnailimage/jpeg5468https://patua.iec.gov.br/bitstreams/9f2f7732-3f07-4104-a20e-e29890452a43/download870d0c3d032bc58291fa180e60e47025MD58ORIGINALEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdfEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdfapplication/pdf213767https://patua.iec.gov.br/bitstreams/037f7dcc-afc2-4f51-b5f6-e05c13b00e9f/download3af30746ca23cf02925fa771d7419e76MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/f6e96723-f662-4c28-94b9-8d3875e2ce37/download52f1732ea66fbd1123abe39f5373b797MD52TEXTevaluation1678-9946-rimtsp-58-00072.pdf.txtevaluation1678-9946-rimtsp-58-00072.pdf.txtExtracted texttext/plain22873https://patua.iec.gov.br/bitstreams/27189bfa-2d65-4fd6-9283-c847daf9e5bf/downloadbd02db0e1114522ae199f100d5274335MD53Evaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.txtEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.txtExtracted texttext/plain22963https://patua.iec.gov.br/bitstreams/f249bbb3-10bd-498c-8fc6-49c007481f0c/downloadbb43671872476c601e834cc3e445faeeMD57iec/25112022-10-20 21:28:27.737oai:patua.iec.gov.br:iec/2511https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:28:27Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
title Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
spellingShingle Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
Gomes, Margarete do Socorro Mendonça
Malária / transmissão
Plasmodium vivax
Mapeamento Cromossômico
Oiapoque (AP)
Brasil (BR)
Guiana Francesa (FR)
title_short Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
title_full Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
title_fullStr Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
title_full_unstemmed Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
title_sort Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
author Gomes, Margarete do Socorro Mendonça
author_facet Gomes, Margarete do Socorro Mendonça
Vieira, José Luiz Fernandes
Cassiano, Gustavo Capatti
Musset, Lise
Legrand, Eric
Nacher, Mathieu
Couto, Vanja Suely Calvosa D’Almeida
Machado, Ricardo Luiz Dantas
Couto, Álvaro Augusto Ribeiro D’Almeida
author_role author
author2 Vieira, José Luiz Fernandes
Cassiano, Gustavo Capatti
Musset, Lise
Legrand, Eric
Nacher, Mathieu
Couto, Vanja Suely Calvosa D’Almeida
Machado, Ricardo Luiz Dantas
Couto, Álvaro Augusto Ribeiro D’Almeida
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Margarete do Socorro Mendonça
Vieira, José Luiz Fernandes
Cassiano, Gustavo Capatti
Musset, Lise
Legrand, Eric
Nacher, Mathieu
Couto, Vanja Suely Calvosa D’Almeida
Machado, Ricardo Luiz Dantas
Couto, Álvaro Augusto Ribeiro D’Almeida
dc.subject.decsPrimary.pt_BR.fl_str_mv Malária / transmissão
Plasmodium vivax
Mapeamento Cromossômico
Oiapoque (AP)
Brasil (BR)
Guiana Francesa (FR)
topic Malária / transmissão
Plasmodium vivax
Mapeamento Cromossômico
Oiapoque (AP)
Brasil (BR)
Guiana Francesa (FR)
description Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana.
publishDate 2016
dc.date.iso.-.fl_str_mv 20170214
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-14T11:06:19Z
dc.date.available.fl_str_mv 2017-02-14T11:06:19Z
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dc.identifier.citation.fl_str_mv GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2511
dc.identifier.issn.-.fl_str_mv 1678-9946
dc.identifier.doi.-.fl_str_mv 10.1590/S1678-9946201658072
identifier_str_mv GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016.
1678-9946
10.1590/S1678-9946201658072
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