Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2511 |
Resumo: | Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana. |
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Gomes, Margarete do Socorro MendonçaVieira, José Luiz FernandesCassiano, Gustavo CapattiMusset, LiseLegrand, EricNacher, MathieuCouto, Vanja Suely Calvosa D’AlmeidaMachado, Ricardo Luiz DantasCouto, Álvaro Augusto Ribeiro D’Almeida2017-02-14T11:06:19Z2017-02-14T11:06:19Z201620170214GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016.1678-9946https://patua.iec.gov.br/handle/iec/251110.1590/S1678-9946201658072Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana.The work reported in this manuscript was funded by the National Scientific and Technological Development in the Research Program for Health System Unique (CNPq PPSUS Public Notice No. 00077/2008/ MCT/CNPq/SETEC, Process No. 35.000.162/2009), and Government of the State of Amapá, and also, Central Public Health Laboratory (LACEN) of AmapáSecretaria de Estado da Saúde do Amapá. Laboratório Central. Macapá, AP, Brazil / Universidade Federal do Pará. Belém, PA, Brazil.Universidade Federal do Pará. Belém, PA, Brazil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, SP, Brazil.Secretaria de Estado da Saúde do Amapá. Macapá, AP, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Pará. Belém, PA, Brazil / Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto, SP, Brazil.Universidade Federal do Pará. Belém, PA, Brazil.Institut Pasteur de la Guyane. Centre National de Référence du Paludisme. Laboratoire de Parasitologie. Cayenne, French Guiana.Institut Pasteur de la Guyane. Centre National de Référence du Paludisme. Laboratoire de Parasitologie. Cayenne, French Guiana.Université des Antilles et de la Guyane. Cayenne, French Guiana.engUniversidade de São PauloEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guianainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária / transmissãoPlasmodium vivaxMapeamento CromossômicoOiapoque (AP)Brasil (BR)Guiana Francesa (FR)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgEvaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgGenerated Thumbnailimage/jpeg1587https://patua.iec.gov.br/bitstreams/43af4ce9-433d-494c-bc8f-d4ec290449fa/downloadfadac5c0aa7a0472ea5cfb10266abbdcMD55Evaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.jpgGenerated Thumbnailimage/jpeg5468https://patua.iec.gov.br/bitstreams/9f2f7732-3f07-4104-a20e-e29890452a43/download870d0c3d032bc58291fa180e60e47025MD58ORIGINALEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdfEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdfapplication/pdf213767https://patua.iec.gov.br/bitstreams/037f7dcc-afc2-4f51-b5f6-e05c13b00e9f/download3af30746ca23cf02925fa771d7419e76MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/f6e96723-f662-4c28-94b9-8d3875e2ce37/download52f1732ea66fbd1123abe39f5373b797MD52TEXTevaluation1678-9946-rimtsp-58-00072.pdf.txtevaluation1678-9946-rimtsp-58-00072.pdf.txtExtracted texttext/plain22873https://patua.iec.gov.br/bitstreams/27189bfa-2d65-4fd6-9283-c847daf9e5bf/downloadbd02db0e1114522ae199f100d5274335MD53Evaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.txtEvaluation of circumsporozoite protein of Plasmodium vivax to stimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana.pdf.txtExtracted texttext/plain22963https://patua.iec.gov.br/bitstreams/f249bbb3-10bd-498c-8fc6-49c007481f0c/downloadbb43671872476c601e834cc3e445faeeMD57iec/25112022-10-20 21:28:27.737oai:patua.iec.gov.br:iec/2511https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:28:27Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
title |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
spellingShingle |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana Gomes, Margarete do Socorro Mendonça Malária / transmissão Plasmodium vivax Mapeamento Cromossômico Oiapoque (AP) Brasil (BR) Guiana Francesa (FR) |
title_short |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
title_full |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
title_fullStr |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
title_full_unstemmed |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
title_sort |
Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana |
author |
Gomes, Margarete do Socorro Mendonça |
author_facet |
Gomes, Margarete do Socorro Mendonça Vieira, José Luiz Fernandes Cassiano, Gustavo Capatti Musset, Lise Legrand, Eric Nacher, Mathieu Couto, Vanja Suely Calvosa D’Almeida Machado, Ricardo Luiz Dantas Couto, Álvaro Augusto Ribeiro D’Almeida |
author_role |
author |
author2 |
Vieira, José Luiz Fernandes Cassiano, Gustavo Capatti Musset, Lise Legrand, Eric Nacher, Mathieu Couto, Vanja Suely Calvosa D’Almeida Machado, Ricardo Luiz Dantas Couto, Álvaro Augusto Ribeiro D’Almeida |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes, Margarete do Socorro Mendonça Vieira, José Luiz Fernandes Cassiano, Gustavo Capatti Musset, Lise Legrand, Eric Nacher, Mathieu Couto, Vanja Suely Calvosa D’Almeida Machado, Ricardo Luiz Dantas Couto, Álvaro Augusto Ribeiro D’Almeida |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária / transmissão Plasmodium vivax Mapeamento Cromossômico Oiapoque (AP) Brasil (BR) Guiana Francesa (FR) |
topic |
Malária / transmissão Plasmodium vivax Mapeamento Cromossômico Oiapoque (AP) Brasil (BR) Guiana Francesa (FR) |
description |
Malaria is a major health problem for people who live on the border between Brazil and French Guiana. Here we discuss Plasmodium vivax distribution pattern in the town of Oiapoque, Amapá State using the circumsporozoite (CS) gene as a marker. Ninety-one peripheral blood samples from P. vivax patients have been studied. Of these, 64 individuals were from the municipality of Oiapoque (Amapá State, Brazil) and 27 patients from French Guiana (August to December 2011). DNA extraction was performed, and a fragment of the P. vivax CS gene was subsequently analyzed using PCR/RFLP. The VK210 genotype was the most common in both countries (48.36% in Brazil and 14.28% in French Guiana), followed by the P. vivax-like (1.10% in both Brazil and French Guiana) and VK247 (1.10% only in Brazil) in single infections. We were able to detect all three CS genotypes simultaneously in mixed infections. There were no statistically significant differences either regarding infection site or parasitaemia among individuals with different genotypes. These results suggest that the same genotypes circulating in French Guiana are found in the municipality of Oiapoque in Brazil. These findings suggest that there may be a dispersion of parasitic populations occurring between the two countries. Most likely, this distribution is associated with prolonged and/or more complex transmission patterns of these genotypes in Brazil, bordering French Guiana. |
publishDate |
2016 |
dc.date.iso.-.fl_str_mv |
20170214 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-02-14T11:06:19Z |
dc.date.available.fl_str_mv |
2017-02-14T11:06:19Z |
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GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2511 |
dc.identifier.issn.-.fl_str_mv |
1678-9946 |
dc.identifier.doi.-.fl_str_mv |
10.1590/S1678-9946201658072 |
identifier_str_mv |
GOMES, Margarete do Socorro Mendonça et al. Evaluation of circumsporozoite protein of Plasmodium vivax to estimate its prevalence in Oiapoque, Amapá State, Brazil, bordering French Guiana. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, p. 72, 2016. 1678-9946 10.1590/S1678-9946201658072 |
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https://patua.iec.gov.br/handle/iec/2511 |
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Universidade de São Paulo |
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Universidade de São Paulo |
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