Biological effects of kojic acid on human monocytes in vitro
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3067 |
Resumo: | Monocytes are mononuclear phagocytes in peripheral blood that can differentiate into macrophages and dendritic cells. Macrophages play a specific role in the inflammatory process and are essential for the innate response. Given the important role of monocytes/macrophages in the immune response, this study aimed to evaluate the activity of kojic acid (KA), a natural product of certain fungal species, on human peripheral blood monocytes in vitro. Purified monocytes isolated from human blood were incubated with KA (50 μg/mL for 48 h) and analyzed by light microscopy, scanning electron microscopy, transmission electron microscopy and flow cytometry. Host cell cytotoxicity was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. KA treatment induced morphological alterations in monocytes, such as increased cell size, as well as numerous cellular projections. Furthermore, flow cytometry revealed increased labeling of cell surface EMR1-F4/80 but decreased labeling of CD11b and CD14. KA also promoted increased IL-6 cytokine production but did not cause cytotoxic effects in monocytes. In conclusion, our results show that KA promotes the differentiation of monocytes into macrophages and can act as an immunomodulatory agent. |
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Costa, J. P. daRodrigues, Ana Paula DrummondFarias, L. H. SFrade, P. C. RSilva, B. J. M. daNascimento, J. L. M. doSilva, E. O2018-03-02T11:56:53Z2018-03-02T11:56:53Z2018COSTA, J. P. da et al. Biological effects of kojic acid on human monocytes in vitro. Biomedicine and Pharmacotherapy, v. 101, p. 100-106, May 2018. DOI: https://doi.org/10.1016/j.biopha.2018.02.0360753-3322https://patua.iec.gov.br/handle/iec/306710.1016/j.biopha.2018.02.036Monocytes are mononuclear phagocytes in peripheral blood that can differentiate into macrophages and dendritic cells. Macrophages play a specific role in the inflammatory process and are essential for the innate response. Given the important role of monocytes/macrophages in the immune response, this study aimed to evaluate the activity of kojic acid (KA), a natural product of certain fungal species, on human peripheral blood monocytes in vitro. Purified monocytes isolated from human blood were incubated with KA (50 μg/mL for 48 h) and analyzed by light microscopy, scanning electron microscopy, transmission electron microscopy and flow cytometry. Host cell cytotoxicity was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. KA treatment induced morphological alterations in monocytes, such as increased cell size, as well as numerous cellular projections. Furthermore, flow cytometry revealed increased labeling of cell surface EMR1-F4/80 but decreased labeling of CD11b and CD14. KA also promoted increased IL-6 cytokine production but did not cause cytotoxic effects in monocytes. In conclusion, our results show that KA promotes the differentiation of monocytes into macrophages and can act as an immunomodulatory agent.Financial support for this study was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PROCAD NF/CAPES), PROPESP-UFPA and the Instituto Nacional de Biologia Estrutural e Bioimagem-INBEB (CNPq - grant number 465395/2014).Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia Eletrônica. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Neurochemistry Molecular and Cellular. Belém, PA, Brazil / University CEUMA. Neuroscience Research Group. São Luís, MA, Brazil / National Institute of Science and Technology in Neuroimmunomodulation. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Structural Biology. Belém, PA, Brazil / National Institute of Science and Technology in Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.engElsevierBiological effects of kojic acid on human monocytes in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAutofagia / imunologiaFagócitos / metabolismoFagocitoseMicroscopia Eletrônica de Varredura / utilizaçãoMicroscopia Eletrônica de Transmissão e Varredura / utilizaçãoMicroscopia Eletrônica de Transmissão / utilizaçãoCitometria de Fluxo / utilizaçãoÁcido Kójico / utilizaçãoinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/737b8169-a696-48a6-aa94-6cd4a86131fe/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/0b88c117-9ce9-46f2-a345-f66a5106fba9/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD56TEXTBiological effects of kojic acid on human monocytes in vitro.pdf.txtBiological effects of kojic acid on human monocytes in vitro.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/fdb07492-98c9-4730-8a2e-9b0e94c034fa/downloade1c06d85ae7b8b032bef47e42e4c08f9MD59Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/3a2ac215-aeb2-42bc-ba77-0b18f8c334c0/downloade1c06d85ae7b8b032bef47e42e4c08f9MD511THUMBNAILBiological effects of kojic acid on human monocytes in vitro.pdf.jpgBiological effects of kojic acid on human monocytes in vitro.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/badf8b08-795d-454b-bc1a-306fa2885e15/download71859d578212107f7f8c49a4ce09d9eeMD510Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/3c79dfa9-d208-46ba-a51e-86fbc3dac19a/download71859d578212107f7f8c49a4ce09d9eeMD512iec/30672023-05-24 11:50:39.641oai:patua.iec.gov.br:iec/3067https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-05-24T11:50:39Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Biological effects of kojic acid on human monocytes in vitro |
title |
Biological effects of kojic acid on human monocytes in vitro |
spellingShingle |
Biological effects of kojic acid on human monocytes in vitro Costa, J. P. da Autofagia / imunologia Fagócitos / metabolismo Fagocitose Microscopia Eletrônica de Varredura / utilização Microscopia Eletrônica de Transmissão e Varredura / utilização Microscopia Eletrônica de Transmissão / utilização Citometria de Fluxo / utilização Ácido Kójico / utilização |
title_short |
Biological effects of kojic acid on human monocytes in vitro |
title_full |
Biological effects of kojic acid on human monocytes in vitro |
title_fullStr |
Biological effects of kojic acid on human monocytes in vitro |
title_full_unstemmed |
Biological effects of kojic acid on human monocytes in vitro |
title_sort |
Biological effects of kojic acid on human monocytes in vitro |
author |
Costa, J. P. da |
author_facet |
Costa, J. P. da Rodrigues, Ana Paula Drummond Farias, L. H. S Frade, P. C. R Silva, B. J. M. da Nascimento, J. L. M. do Silva, E. O |
author_role |
author |
author2 |
Rodrigues, Ana Paula Drummond Farias, L. H. S Frade, P. C. R Silva, B. J. M. da Nascimento, J. L. M. do Silva, E. O |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, J. P. da Rodrigues, Ana Paula Drummond Farias, L. H. S Frade, P. C. R Silva, B. J. M. da Nascimento, J. L. M. do Silva, E. O |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Autofagia / imunologia Fagócitos / metabolismo Fagocitose Microscopia Eletrônica de Varredura / utilização Microscopia Eletrônica de Transmissão e Varredura / utilização Microscopia Eletrônica de Transmissão / utilização Citometria de Fluxo / utilização Ácido Kójico / utilização |
topic |
Autofagia / imunologia Fagócitos / metabolismo Fagocitose Microscopia Eletrônica de Varredura / utilização Microscopia Eletrônica de Transmissão e Varredura / utilização Microscopia Eletrônica de Transmissão / utilização Citometria de Fluxo / utilização Ácido Kójico / utilização |
description |
Monocytes are mononuclear phagocytes in peripheral blood that can differentiate into macrophages and dendritic cells. Macrophages play a specific role in the inflammatory process and are essential for the innate response. Given the important role of monocytes/macrophages in the immune response, this study aimed to evaluate the activity of kojic acid (KA), a natural product of certain fungal species, on human peripheral blood monocytes in vitro. Purified monocytes isolated from human blood were incubated with KA (50 μg/mL for 48 h) and analyzed by light microscopy, scanning electron microscopy, transmission electron microscopy and flow cytometry. Host cell cytotoxicity was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. KA treatment induced morphological alterations in monocytes, such as increased cell size, as well as numerous cellular projections. Furthermore, flow cytometry revealed increased labeling of cell surface EMR1-F4/80 but decreased labeling of CD11b and CD14. KA also promoted increased IL-6 cytokine production but did not cause cytotoxic effects in monocytes. In conclusion, our results show that KA promotes the differentiation of monocytes into macrophages and can act as an immunomodulatory agent. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-03-02T11:56:53Z |
dc.date.available.fl_str_mv |
2018-03-02T11:56:53Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
COSTA, J. P. da et al. Biological effects of kojic acid on human monocytes in vitro. Biomedicine and Pharmacotherapy, v. 101, p. 100-106, May 2018. DOI: https://doi.org/10.1016/j.biopha.2018.02.036 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3067 |
dc.identifier.issn.-.fl_str_mv |
0753-3322 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.biopha.2018.02.036 |
identifier_str_mv |
COSTA, J. P. da et al. Biological effects of kojic acid on human monocytes in vitro. Biomedicine and Pharmacotherapy, v. 101, p. 100-106, May 2018. DOI: https://doi.org/10.1016/j.biopha.2018.02.036 0753-3322 10.1016/j.biopha.2018.02.036 |
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https://patua.iec.gov.br/handle/iec/3067 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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