Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1

Detalhes bibliográficos
Autor(a) principal: Hernandez, Juliana das Merces
Data de Publicação: 2016
Outros Autores: Silva, Luciana Damascena da, Sousa Junior, Edivaldo Costa, Lucena, Maria Silvia Souza de, Soares, Luana da Silva, Mascarenhas, Joana D'Arc Pereira, Gabbay, Yvone Benchimol
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2131
Resumo: Norovirus (NoV) is responsible for outbreaks and sporadic cases of nonbacterial acute gastroenteritis in humans worldwide. The virus consists of small round particles containing a single-stranded RNA genome that is divided into three Open Reading Frames. NoV evolves via mechanisms of antigenic drift and recombination, which lead to the emergence of new strains that are capable of causing global epidemics. Recombination usually occurs in the ORF1/ORF2 overlapping region and generates strains with different genotypes in the polymerase and capsid region. The primary objective of this study was to analyze recombination in positive-NoV samples. Specimens were collected during 2011, 2012 and 2014, from children under two years of age presenting gastrointestinal symptoms such as vomiting and diarrhea. The partial polymerase (B region), capsid (D region) genes and the ORF1-ORF2 overlap regions were sequenced in each sample. The recombinant analyses were performed in the Simplot software v.3.5.1 and RDP4 Beta v. 4.6 program. These analyses showed that GII.Pg/GII.1, GII.P7/GII.6, and GII.P22/GII.5 were recombinant strains. To our knowledge, this is the first time that the GII.P22/GII.5 and GII.Pg/GII.1 strains were described in South America and the GII.P7/GII.6 was detected in Northern of Brazil.
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spelling Hernandez, Juliana das MercesSilva, Luciana Damascena daSousa Junior, Edivaldo CostaLucena, Maria Silvia Souza deSoares, Luana da SilvaMascarenhas, Joana D'Arc PereiraGabbay, Yvone Benchimol2016-06-09T12:04:43Z2016-06-09T12:04:43Z2016HERNANDEZ, Juliana das Merces et al. Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1. Infection, Genetics and Evolution, v. 39, p. 365-371, Apr. 2016. DOI: https://doi.org/10.1016/j.meegid.2016.02.0071567-7257https://patua.iec.gov.br/handle/iec/213110.1016/j.meegid.2016.02.007Norovirus (NoV) is responsible for outbreaks and sporadic cases of nonbacterial acute gastroenteritis in humans worldwide. The virus consists of small round particles containing a single-stranded RNA genome that is divided into three Open Reading Frames. NoV evolves via mechanisms of antigenic drift and recombination, which lead to the emergence of new strains that are capable of causing global epidemics. Recombination usually occurs in the ORF1/ORF2 overlapping region and generates strains with different genotypes in the polymerase and capsid region. The primary objective of this study was to analyze recombination in positive-NoV samples. Specimens were collected during 2011, 2012 and 2014, from children under two years of age presenting gastrointestinal symptoms such as vomiting and diarrhea. The partial polymerase (B region), capsid (D region) genes and the ORF1-ORF2 overlap regions were sequenced in each sample. The recombinant analyses were performed in the Simplot software v.3.5.1 and RDP4 Beta v. 4.6 program. These analyses showed that GII.Pg/GII.1, GII.P7/GII.6, and GII.P22/GII.5 were recombinant strains. To our knowledge, this is the first time that the GII.P22/GII.5 and GII.Pg/GII.1 strains were described in South America and the GII.P7/GII.6 was detected in Northern of Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
dc.title.alternative.pt_BR.fl_str_mv Análise de recombinantes de norovírus incomuns de Manaus, Amazon região, Brasil: GII. P22 / GII. 5, GII. P7 / GII. 6 e GII. Pg / GII. 1
title Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
spellingShingle Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
Hernandez, Juliana das Merces
Norovirus / genética
Fezes / virologia
Filogenia
Reação em Cadeia da Polimerase / métodos
Manaus (AM)
title_short Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
title_full Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
title_fullStr Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
title_full_unstemmed Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
title_sort Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1
author Hernandez, Juliana das Merces
author_facet Hernandez, Juliana das Merces
Silva, Luciana Damascena da
Sousa Junior, Edivaldo Costa
Lucena, Maria Silvia Souza de
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
Gabbay, Yvone Benchimol
author_role author
author2 Silva, Luciana Damascena da
Sousa Junior, Edivaldo Costa
Lucena, Maria Silvia Souza de
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
Gabbay, Yvone Benchimol
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hernandez, Juliana das Merces
Silva, Luciana Damascena da
Sousa Junior, Edivaldo Costa
Lucena, Maria Silvia Souza de
Soares, Luana da Silva
Mascarenhas, Joana D'Arc Pereira
Gabbay, Yvone Benchimol
dc.subject.decsPrimary.pt_BR.fl_str_mv Norovirus / genética
Fezes / virologia
Filogenia
Reação em Cadeia da Polimerase / métodos
Manaus (AM)
topic Norovirus / genética
Fezes / virologia
Filogenia
Reação em Cadeia da Polimerase / métodos
Manaus (AM)
description Norovirus (NoV) is responsible for outbreaks and sporadic cases of nonbacterial acute gastroenteritis in humans worldwide. The virus consists of small round particles containing a single-stranded RNA genome that is divided into three Open Reading Frames. NoV evolves via mechanisms of antigenic drift and recombination, which lead to the emergence of new strains that are capable of causing global epidemics. Recombination usually occurs in the ORF1/ORF2 overlapping region and generates strains with different genotypes in the polymerase and capsid region. The primary objective of this study was to analyze recombination in positive-NoV samples. Specimens were collected during 2011, 2012 and 2014, from children under two years of age presenting gastrointestinal symptoms such as vomiting and diarrhea. The partial polymerase (B region), capsid (D region) genes and the ORF1-ORF2 overlap regions were sequenced in each sample. The recombinant analyses were performed in the Simplot software v.3.5.1 and RDP4 Beta v. 4.6 program. These analyses showed that GII.Pg/GII.1, GII.P7/GII.6, and GII.P22/GII.5 were recombinant strains. To our knowledge, this is the first time that the GII.P22/GII.5 and GII.Pg/GII.1 strains were described in South America and the GII.P7/GII.6 was detected in Northern of Brazil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-09T12:04:43Z
dc.date.available.fl_str_mv 2016-06-09T12:04:43Z
dc.date.issued.fl_str_mv 2016
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dc.identifier.citation.fl_str_mv HERNANDEZ, Juliana das Merces et al. Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1. Infection, Genetics and Evolution, v. 39, p. 365-371, Apr. 2016. DOI: https://doi.org/10.1016/j.meegid.2016.02.007
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2131
dc.identifier.issn.-.fl_str_mv 1567-7257
dc.identifier.doi.-.fl_str_mv 10.1016/j.meegid.2016.02.007
identifier_str_mv HERNANDEZ, Juliana das Merces et al. Analysis of uncommon norovirus recombinants from Manaus, Amazon region, Brazil: GII. P22 / GII. 5, GII. P7 / GII. 6 and GII. Pg / GII. 1. Infection, Genetics and Evolution, v. 39, p. 365-371, Apr. 2016. DOI: https://doi.org/10.1016/j.meegid.2016.02.007
1567-7257
10.1016/j.meegid.2016.02.007
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