Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12

Detalhes bibliográficos
Autor(a) principal: Portal, Thayara Morais
Data de Publicação: 2016
Outros Autores: Siqueira, Jones Anderson Monteiro, Costa, Larissa Cristina Prado das Neves, Lima, Ian Carlos Gomes de, Lucena, Maria Silvia Sousa de, Bandeira, Renato da Silva, Linhares, Alexandre da Costa, Luz, Claudia Regina Nunes Eloi da, Gabbay, Yvone Benchimol, Resque, Hugo Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2480
Resumo: Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.
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spelling Portal, Thayara MoraisSiqueira, Jones Anderson MonteiroCosta, Larissa Cristina Prado das NevesLima, Ian Carlos Gomes deLucena, Maria Silvia Sousa deBandeira, Renato da SilvaLinhares, Alexandre da CostaLuz, Claudia Regina Nunes Eloi daGabbay, Yvone BenchimolResque, Hugo Reis2017-04-11T17:25:31Z2017-04-11T17:25:31Z2016PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016.1678-4405https://patua.iec.gov.br/handle/iec/248010.1016/j.bjm.2016.04.008Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Faculdade Integrada Brasil Amazônia. Belém, Pará, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Maranhão. São Luís, Maranhão, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
title Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
spellingShingle Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
Portal, Thayara Morais
Sapovirus / genética
Fezes / virologia
Genótipo
Norovirus / genética
Variação Genética
Diarreia Infantil / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Brasil (BR)
title_short Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
title_full Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
title_fullStr Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
title_full_unstemmed Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
title_sort Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
author Portal, Thayara Morais
author_facet Portal, Thayara Morais
Siqueira, Jones Anderson Monteiro
Costa, Larissa Cristina Prado das Neves
Lima, Ian Carlos Gomes de
Lucena, Maria Silvia Sousa de
Bandeira, Renato da Silva
Linhares, Alexandre da Costa
Luz, Claudia Regina Nunes Eloi da
Gabbay, Yvone Benchimol
Resque, Hugo Reis
author_role author
author2 Siqueira, Jones Anderson Monteiro
Costa, Larissa Cristina Prado das Neves
Lima, Ian Carlos Gomes de
Lucena, Maria Silvia Sousa de
Bandeira, Renato da Silva
Linhares, Alexandre da Costa
Luz, Claudia Regina Nunes Eloi da
Gabbay, Yvone Benchimol
Resque, Hugo Reis
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Portal, Thayara Morais
Siqueira, Jones Anderson Monteiro
Costa, Larissa Cristina Prado das Neves
Lima, Ian Carlos Gomes de
Lucena, Maria Silvia Sousa de
Bandeira, Renato da Silva
Linhares, Alexandre da Costa
Luz, Claudia Regina Nunes Eloi da
Gabbay, Yvone Benchimol
Resque, Hugo Reis
dc.subject.decsPrimary.pt_BR.fl_str_mv Sapovirus / genética
Fezes / virologia
Genótipo
Norovirus / genética
Variação Genética
Diarreia Infantil / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Brasil (BR)
topic Sapovirus / genética
Fezes / virologia
Genótipo
Norovirus / genética
Variação Genética
Diarreia Infantil / virologia
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Brasil (BR)
description Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-04-11T17:25:31Z
dc.date.available.fl_str_mv 2017-04-11T17:25:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2480
dc.identifier.issn.-.fl_str_mv 1678-4405
dc.identifier.doi.-.fl_str_mv 10.1016/j.bjm.2016.04.008
identifier_str_mv PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016.
1678-4405
10.1016/j.bjm.2016.04.008
url https://patua.iec.gov.br/handle/iec/2480
dc.language.iso.fl_str_mv eng
language eng
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dc.format.none.fl_str_mv application/pdf
dc.coverage.temporalragefrom.-.fl_str_mv 1997
dc.coverage.temporalrageupto.-.fl_str_mv 1999
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
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