Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2480 |
Resumo: | Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão. |
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Portal, Thayara MoraisSiqueira, Jones Anderson MonteiroCosta, Larissa Cristina Prado das NevesLima, Ian Carlos Gomes deLucena, Maria Silvia Sousa deBandeira, Renato da SilvaLinhares, Alexandre da CostaLuz, Claudia Regina Nunes Eloi daGabbay, Yvone BenchimolResque, Hugo Reis2017-04-11T17:25:31Z2017-04-11T17:25:31Z2016PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016.1678-4405https://patua.iec.gov.br/handle/iec/248010.1016/j.bjm.2016.04.008Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão.Universidade do Estado do Pará. Programa de Pós-Graduação em Biologia Parasitária na Amazônia. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Faculdade Integrada Brasil Amazônia. Belém, Pará, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Maranhão. São Luís, Maranhão, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.application/pdfengSociedade Brasileira de MicrobiologiaCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19971999Sapovirus / genéticaFezes / virologiaGenótipoNorovirus / genéticaVariação GenéticaDiarreia Infantil / virologiaEnsaio de Imunoadsorção Enzimática / métodosReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosBrasil (BR)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12.pdfapplication/pdf930963https://patua.iec.gov.br/bitstreams/ddc88c82-49eb-484d-a7fd-d0f621b37bca/downloadd2d48543d05e5eb24750aa4889761291MD51TEXTCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12.pdf.txtCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12.pdf.txtExtracted texttext/plain100307https://patua.iec.gov.br/bitstreams/16ca7a14-e27c-420e-9cd6-79a3a1c991a7/download3c1aca54d511921c3307c63cf4d0d056MD55THUMBNAILCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12.pdf.jpgCaliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12.pdf.jpgGenerated Thumbnailimage/jpeg5784https://patua.iec.gov.br/bitstreams/722c249c-8529-4fc6-bd8a-0a7493ae0364/downloadb36ae69082bc27c15f1390e97fd01f89MD56LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
title |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
spellingShingle |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 Portal, Thayara Morais Sapovirus / genética Fezes / virologia Genótipo Norovirus / genética Variação Genética Diarreia Infantil / virologia Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Brasil (BR) |
title_short |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
title_full |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
title_fullStr |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
title_full_unstemmed |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
title_sort |
Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12 |
author |
Portal, Thayara Morais |
author_facet |
Portal, Thayara Morais Siqueira, Jones Anderson Monteiro Costa, Larissa Cristina Prado das Neves Lima, Ian Carlos Gomes de Lucena, Maria Silvia Sousa de Bandeira, Renato da Silva Linhares, Alexandre da Costa Luz, Claudia Regina Nunes Eloi da Gabbay, Yvone Benchimol Resque, Hugo Reis |
author_role |
author |
author2 |
Siqueira, Jones Anderson Monteiro Costa, Larissa Cristina Prado das Neves Lima, Ian Carlos Gomes de Lucena, Maria Silvia Sousa de Bandeira, Renato da Silva Linhares, Alexandre da Costa Luz, Claudia Regina Nunes Eloi da Gabbay, Yvone Benchimol Resque, Hugo Reis |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Portal, Thayara Morais Siqueira, Jones Anderson Monteiro Costa, Larissa Cristina Prado das Neves Lima, Ian Carlos Gomes de Lucena, Maria Silvia Sousa de Bandeira, Renato da Silva Linhares, Alexandre da Costa Luz, Claudia Regina Nunes Eloi da Gabbay, Yvone Benchimol Resque, Hugo Reis |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Sapovirus / genética Fezes / virologia Genótipo Norovirus / genética Variação Genética Diarreia Infantil / virologia Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Brasil (BR) |
topic |
Sapovirus / genética Fezes / virologia Genótipo Norovirus / genética Variação Genética Diarreia Infantil / virologia Ensaio de Imunoadsorção Enzimática / métodos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Brasil (BR) |
description |
Gastroenteritis is one of the most common diseases during childhood, with norovirus (NoV) and sapovirus (SaV) being two of its main causes. This study reports for the first time the incidence of these viruses in hospitalized children with and without gastroenteritis in São Luís, Maranhão. A total of 136 fecal samples were tested by enzyme immunoassays (EIA) for the detection of NoV and by reverse transcription-polymerase chain reaction (RT-PCR) for detection of both NoV and SaV. Positive samples for both agents were subjected to sequencing. The overall frequency of NoV as detected by EIA and RT-PCR was 17.6 percent (24/136) and 32.6 percent (15/46), respectively in diarrheic patients and 10.0 percent (9/90) in non-diarrheic patients (p < 0.01). Of the diarrheic patients, 17 percent had fever, vomiting and anorexia, and 13 percent developed fever, vomiting and abdominal pain. Of the 24 NoV-positive samples, 50 percent (12/24) were sequenced and classified as genotypes GII.3 (n = 1), GII.4 (6), GII.5 (1), GII.7 (2), GII.12 (1) and GII.16 (1). SaV frequency was 9.8 percent (11/112), with 22.6 percent (7/31) in diarrheic patients and 4.9 percent (4/81) in nondiarrheic (p = 0.04) ones. In diarrheic cases, 27.3 percent had fever, vomiting and anorexia, whereas 18.2 percent had fever, anorexia and abdominal pain. One SaV-positive sample was sequenced and classified as GII.1. These results show a high genetic diversity of NoV and higher prevalence of NoV compared to SaV. Our data highlight the importance of NoV and SaV as enteropathogens in São Luís, Maranhão. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-04-11T17:25:31Z |
dc.date.available.fl_str_mv |
2017-04-11T17:25:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2480 |
dc.identifier.issn.-.fl_str_mv |
1678-4405 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.bjm.2016.04.008 |
identifier_str_mv |
PORTAL, Thayara Morais et al. Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999 : detection of norovirus GII.12. Brazilian Journal of Microbiology, v. 47, . 3, p. 724–730, July - Sept. 2016. 1678-4405 10.1016/j.bjm.2016.04.008 |
url |
https://patua.iec.gov.br/handle/iec/2480 |
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eng |
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eng |
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openAccess |
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dc.coverage.temporalrageupto.-.fl_str_mv |
1999 |
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Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
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