Temporal and spatial distribution of the variants of merozoite surface protein-1 (MSP-1) in Plasmodium falciparum populations in Brazil

• Autor(a) principal: Silva, N. S
• Data de Publicação: 2000
• Outros Autores: Silveira, L. A, Machado, Ricardo Luiz Dantas, Póvoa, Marinete Marins, Ferreira, Marcelo Urbano
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/1054
• topic: Proteína 1 de Superfície de Merozoito / análise; Proteína 1 de Superfície de Merozoito / provisão & distribuição; Plasmodium falciparum; Brazil (BR)

The polymorphic, merozoite surface protein-l (MSP-l) of Plasmodium falciparum, an antigen of the parasite's asexual blood-stages, is a major malaria-vaccine candidate. Nucleotide sequences of each variable domain or block of this antigen may be grouped into one of three possible allelic types (Kl, MAD20 and RO33), and 24 major types of the msp-J gene may be defined, as unique combinations of allelic types in these variable blocks. Isolates collected from the Brazilian Amazon, over a period of 14 years, have now bcen invcstigated, by PCR-based typing of the msp-J gene. Thirteen of the 24 possible gene-types were identified, and 336 P. falciparum cloncs were fully typed among 239 isolates. Most parasites (87%) belonged to one of the seven most frequent gene-types. Marked temporal variation in the distribution of msp-J variants was found when comparing parasites samplcd in the same sites at intervals of at least 5 years. Spatial variations were also found when comparing parasites from both neighbouring and distant sites within the Amazon Basin. The between-population variance in the frequencies of msp-J allelic types found in Brazil, as estimated by Wright's FST statistic, is of similar magnitude to that found in previous world-wide comparisons. The potential implications of these findings for the development of an MSP-lbased, multivalent malaria vaccine are discussed.