Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil

• Autor(a) principal: Leão, Sylvia Cardoso
• Data de Publicação: 2010
• Outros Autores: Viana Niero, Cristina, Matsumoto, Cristianne Kayoko, Lima, Karla Valeria Batista, Lopes, Maria Luiza, Palaci, Moisés, Hadad, David Jamil, Vinhas, Solange, Duarte, Rafael Silva, Lourenço, Maria Cristina Silva, Kipnis, André, Neves, Zilah Cândida das, Gabardo, Betina Mendez Alcântara, Ribeiro, Marta Osório, Baethgen, Ludmila, Assis, Denise Brandão de, Madalosso, Geraldine, Chimara, Erica, Dalcolmo, Margareth Pretti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2735
• topic: Infecções por Mycobacterium / epidemiologia; Mycobacterium / classificação; Infecção Hospitalar; Impressões Digitais de DNA; Infecção da Ferida Operatória; Reação em Cadeia da Polimerase / métodos; Brasil / epidemiologia

Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.