Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil

Detalhes bibliográficos
Autor(a) principal: Leão, Sylvia Cardoso
Data de Publicação: 2010
Outros Autores: Viana Niero, Cristina, Matsumoto, Cristianne Kayoko, Lima, Karla Valeria Batista, Lopes, Maria Luiza, Palaci, Moisés, Hadad, David Jamil, Vinhas, Solange, Duarte, Rafael Silva, Lourenço, Maria Cristina Silva, Kipnis, André, Neves, Zilah Cândida das, Gabardo, Betina Mendez Alcântara, Ribeiro, Marta Osório, Baethgen, Ludmila, Assis, Denise Brandão de, Madalosso, Geraldine, Chimara, Erica, Dalcolmo, Margareth Pretti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2735
Resumo: Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.
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spelling Leão, Sylvia CardosoViana Niero, CristinaMatsumoto, Cristianne KayokoLima, Karla Valeria BatistaLopes, Maria LuizaPalaci, MoisésHadad, David JamilVinhas, SolangeDuarte, Rafael SilvaLourenço, Maria Cristina SilvaKipnis, AndréNeves, Zilah Cândida dasGabardo, Betina Mendez AlcântaraRibeiro, Marta OsórioBaethgen, LudmilaAssis, Denise Brandão deMadalosso, GeraldineChimara, EricaDalcolmo, Margareth Pretti2017-09-13T09:41:53Z2017-09-13T09:41:53Z2010LEÃO, Sylvia Cardoso et al. Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil. Future Microbiology. v. 5, n. 6, p. 971 - 980, June 2010.1746-0921https://patua.iec.gov.br/handle/iec/273510.2217/fmb.10.49.Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.Foundation for Research Support of the State of SaoPaulo (FAPESP – 06/1533-9) and the National Council for Scientific and Technological Development (CNPq – Universal 470594/2006-3 and 475238/2008-7).Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil.Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Rio de Janeiro, RJ, Brazil.Universidade Federal de Goiás. Departamento de Microbiologia, Imunologia, Parasitologia e Patologia. Goiânia, GO, Brazil.Secretaria Municipal de Saúde de Goiânia. Goiânia, GO, Brazil.Secretaria Estadual da Saúde do Paraná. Curitiba, PR, Brazil.Laboratório Central de Saúde Pública. Porto Alegre, RS, Brazil.Laboratório Central de Saúde Pública. Porto Alegre, RS, Brazil.Centro de Vigilância Epidemiológica Professor Alexandre Vranjac. São Paulo, SP, Brazil.Centro de Vigilância Epidemiológica Professor Alexandre Vranjac. São Paulo, SP, Brazil.Instituto Adolfo Lutz. Setor de Micobactérias. São Paulo, SP, Brazil.Centro de Referencia Professor Hélio Fraga. Rio de Janeiro, RJ, Brazil.engFuture MedicineEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20042008Infecções por Mycobacterium / epidemiologiaMycobacterium / classificaçãoInfecção HospitalarImpressões Digitais de DNAInfecção da Ferida OperatóriaReação em Cadeia da Polimerase / métodosBrasil / epidemiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdfEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdfapplication/pdf903195https://patua.iec.gov.br/bitstreams/9f654579-955a-4f11-aafb-427764c1422a/download74b18230fda3b5f4c22d02609ee23e37MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/21ef5b53-c9f5-46ea-ae7d-debe8d0be8d9/download52f1732ea66fbd1123abe39f5373b797MD52TEXTEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdf.txtEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdf.txtExtracted texttext/plain40503https://patua.iec.gov.br/bitstreams/7576b904-a091-4bf3-a006-499d90d2b2ef/download79e7c45ae019b2b2937474b6d6836b0fMD55THUMBNAILEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdf.jpgEpidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5663https://patua.iec.gov.br/bitstreams/35fce00c-c8bc-461c-831c-1202a10845e9/download7b8eea8cbe1ff6271821ca78862ac6ecMD56iec/27352022-10-20 23:31:25.196oai:patua.iec.gov.br:iec/2735https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:31:25Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
title Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
spellingShingle Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
Leão, Sylvia Cardoso
Infecções por Mycobacterium / epidemiologia
Mycobacterium / classificação
Infecção Hospitalar
Impressões Digitais de DNA
Infecção da Ferida Operatória
Reação em Cadeia da Polimerase / métodos
Brasil / epidemiologia
title_short Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
title_full Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
title_fullStr Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
title_full_unstemmed Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
title_sort Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil
author Leão, Sylvia Cardoso
author_facet Leão, Sylvia Cardoso
Viana Niero, Cristina
Matsumoto, Cristianne Kayoko
Lima, Karla Valeria Batista
Lopes, Maria Luiza
Palaci, Moisés
Hadad, David Jamil
Vinhas, Solange
Duarte, Rafael Silva
Lourenço, Maria Cristina Silva
Kipnis, André
Neves, Zilah Cândida das
Gabardo, Betina Mendez Alcântara
Ribeiro, Marta Osório
Baethgen, Ludmila
Assis, Denise Brandão de
Madalosso, Geraldine
Chimara, Erica
Dalcolmo, Margareth Pretti
author_role author
author2 Viana Niero, Cristina
Matsumoto, Cristianne Kayoko
Lima, Karla Valeria Batista
Lopes, Maria Luiza
Palaci, Moisés
Hadad, David Jamil
Vinhas, Solange
Duarte, Rafael Silva
Lourenço, Maria Cristina Silva
Kipnis, André
Neves, Zilah Cândida das
Gabardo, Betina Mendez Alcântara
Ribeiro, Marta Osório
Baethgen, Ludmila
Assis, Denise Brandão de
Madalosso, Geraldine
Chimara, Erica
Dalcolmo, Margareth Pretti
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leão, Sylvia Cardoso
Viana Niero, Cristina
Matsumoto, Cristianne Kayoko
Lima, Karla Valeria Batista
Lopes, Maria Luiza
Palaci, Moisés
Hadad, David Jamil
Vinhas, Solange
Duarte, Rafael Silva
Lourenço, Maria Cristina Silva
Kipnis, André
Neves, Zilah Cândida das
Gabardo, Betina Mendez Alcântara
Ribeiro, Marta Osório
Baethgen, Ludmila
Assis, Denise Brandão de
Madalosso, Geraldine
Chimara, Erica
Dalcolmo, Margareth Pretti
dc.subject.decsPrimary.pt_BR.fl_str_mv Infecções por Mycobacterium / epidemiologia
Mycobacterium / classificação
Infecção Hospitalar
Impressões Digitais de DNA
Infecção da Ferida Operatória
Reação em Cadeia da Polimerase / métodos
Brasil / epidemiologia
topic Infecções por Mycobacterium / epidemiologia
Mycobacterium / classificação
Infecção Hospitalar
Impressões Digitais de DNA
Infecção da Ferida Operatória
Reação em Cadeia da Polimerase / métodos
Brasil / epidemiologia
description Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2017-09-13T09:41:53Z
dc.date.available.fl_str_mv 2017-09-13T09:41:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv LEÃO, Sylvia Cardoso et al. Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil. Future Microbiology. v. 5, n. 6, p. 971 - 980, June 2010.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2735
dc.identifier.issn.-.fl_str_mv 1746-0921
dc.identifier.doi.-.fl_str_mv 10.2217/fmb.10.49.
identifier_str_mv LEÃO, Sylvia Cardoso et al. Epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in Brazil. Future Microbiology. v. 5, n. 6, p. 971 - 980, June 2010.
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