Expression pattern of Cdkn2b and its regulators in canine mammary tumors

Detalhes bibliográficos
Autor(a) principal: Faro, Thamirys Aline Silva
Data de Publicação: 2018
Outros Autores: Pinheiro, Danilo do Rosário, Calgagno, Danielle Queiroz, Pereira, Washington Luiz Assunção, Aguirra, Lucien Roberta Valente Miranda de, Burbano, Rommel Rodrigues, Harada, Maria Lúcia, Borges, Bárbara do Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3533
Resumo: Background/Aim: In female dogs, mammary cancer is the most frequent cancer type, accounting for 50% of all tumors affecting these animals. Amongst the commonly altered genes in cancer is the cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b), whose expression is negatively regulated by protein products of BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc) and T-box gene transcription factor 2 (Tbx2) genes. Considering this, the aim of this study was to evaluate the expression pattern of the Cdkn2b gene and these regulators in canine mammary tumors of dogs from Northern Brazil (Belém, Pará). Material and Methods: Gene expression in samples from 33 animals was assessed by real-time polymerase chain reaction. To check the influence of methylation on gene expression, bisulfite sequencing polymerase chain reaction was also performed. Results: All studied genes, except Cdkn2b, were found at increased expression levels in tumor tissue when compared with control samples. No correlation between expression and methylation data was observed. Conclusion: Our results suggest these markers may have a diagnostic value in the veterinary clinic.
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spelling Faro, Thamirys Aline SilvaPinheiro, Danilo do RosárioCalgagno, Danielle QueirozPereira, Washington Luiz AssunçãoAguirra, Lucien Roberta Valente Miranda deBurbano, Rommel RodriguesHarada, Maria LúciaBorges, Bárbara do Nascimento2018-11-22T13:44:01Z2018-11-22T13:44:01Z2018FARO, Thamirys Aline Silva et al. Expression pattern of Cdkn2b and its regulators in canine mammary tumors. Anticancer Research, v. 38, n. 11, p. 6333-6338, Nov. 2018. DOI: https://doi.org/10.21873/anticanres.12991. Disponível em: https://ar.iiarjournals.org/content/anticanres/38/11/6333.full.pdf.0250-7005https://patua.iec.gov.br/handle/iec/353310.21873/anticanres.12991.Background/Aim: In female dogs, mammary cancer is the most frequent cancer type, accounting for 50% of all tumors affecting these animals. Amongst the commonly altered genes in cancer is the cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b), whose expression is negatively regulated by protein products of BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc) and T-box gene transcription factor 2 (Tbx2) genes. Considering this, the aim of this study was to evaluate the expression pattern of the Cdkn2b gene and these regulators in canine mammary tumors of dogs from Northern Brazil (Belém, Pará). Material and Methods: Gene expression in samples from 33 animals was assessed by real-time polymerase chain reaction. To check the influence of methylation on gene expression, bisulfite sequencing polymerase chain reaction was also performed. Results: All studied genes, except Cdkn2b, were found at increased expression levels in tumor tissue when compared with control samples. No correlation between expression and methylation data was observed. Conclusion: Our results suggest these markers may have a diagnostic value in the veterinary clinic.Federal University of Pará. Biological Science Institute. Molecular Biology Laboratory. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.Federal University of Pará. Biological Science Institute. Molecular Biology Laboratory. Belém, PA, Brazil.Federal University of Pará. Oncology Research Center. Belem, PA, Brazil.Federal Rural University of Amazonia. Health and Animal Production Institute. Animal Pathology Laboratory. Belém, PA, Brazil.Federal Rural University of Amazonia. Health and Animal Production Institute. Animal Pathology Laboratory. Belém, PA, Brazil.Ophir Loyola Hospital. Molecular Biology Laboratory. Belém, PA, Brazil / Federal University of Pará. Molecular Biology and Human Cytogenetics Laboratory. Belém, PA, Brazil.Federal University of Pará. Biological Science Institute. Molecular Biology Laboratory. Belém, PA, Brazil.Federal University of Pará. Biological Science Institute. Molecular Biology Laboratory. Belém, PA, Brazil.engInternational Institute of Anticancer ResearchExpression pattern of Cdkn2b and its regulators in canine mammary tumorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeoplasias / patologiaNeoplasias / veterináriaNeoplasias da Mama / veterináriaExpressão Gênica / genéticaRegulação da Expressão Gênicainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/2e3ac4a0-9236-4666-876d-e9ba224c32a2/download52f1732ea66fbd1123abe39f5373b797MD52TEXTExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdf.txtExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdf.txtExtracted texttext/plain27654https://patua.iec.gov.br/bitstreams/d9c6500c-4d95-4810-9a5f-eaaf9f578956/download9b6786c0d1fcedb4fb35d7d95e16fb87MD56THUMBNAILExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdf.jpgExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdf.jpgGenerated Thumbnailimage/jpeg5706https://patua.iec.gov.br/bitstreams/a19dee90-b86b-4f96-b44a-f6aeefe3bd37/download9c751ca8dd38128bd96d51bb718e065cMD57ORIGINALExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdfExpression pattern of Cdkn2b and its regulators in canine mammary tumors.pdfapplication/pdf184366https://patua.iec.gov.br/bitstreams/bb9aaad5-15e8-4262-b3af-9ce63fb02fe3/downloadd9f84ea5b736c525aa5d85ad4ac6dec5MD55iec/35332022-10-20 22:11:33.939oai:patua.iec.gov.br:iec/3533https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:11:33Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Expression pattern of Cdkn2b and its regulators in canine mammary tumors
title Expression pattern of Cdkn2b and its regulators in canine mammary tumors
spellingShingle Expression pattern of Cdkn2b and its regulators in canine mammary tumors
Faro, Thamirys Aline Silva
Neoplasias / patologia
Neoplasias / veterinária
Neoplasias da Mama / veterinária
Expressão Gênica / genética
Regulação da Expressão Gênica
title_short Expression pattern of Cdkn2b and its regulators in canine mammary tumors
title_full Expression pattern of Cdkn2b and its regulators in canine mammary tumors
title_fullStr Expression pattern of Cdkn2b and its regulators in canine mammary tumors
title_full_unstemmed Expression pattern of Cdkn2b and its regulators in canine mammary tumors
title_sort Expression pattern of Cdkn2b and its regulators in canine mammary tumors
author Faro, Thamirys Aline Silva
author_facet Faro, Thamirys Aline Silva
Pinheiro, Danilo do Rosário
Calgagno, Danielle Queiroz
Pereira, Washington Luiz Assunção
Aguirra, Lucien Roberta Valente Miranda de
Burbano, Rommel Rodrigues
Harada, Maria Lúcia
Borges, Bárbara do Nascimento
author_role author
author2 Pinheiro, Danilo do Rosário
Calgagno, Danielle Queiroz
Pereira, Washington Luiz Assunção
Aguirra, Lucien Roberta Valente Miranda de
Burbano, Rommel Rodrigues
Harada, Maria Lúcia
Borges, Bárbara do Nascimento
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Faro, Thamirys Aline Silva
Pinheiro, Danilo do Rosário
Calgagno, Danielle Queiroz
Pereira, Washington Luiz Assunção
Aguirra, Lucien Roberta Valente Miranda de
Burbano, Rommel Rodrigues
Harada, Maria Lúcia
Borges, Bárbara do Nascimento
dc.subject.decsPrimary.pt_BR.fl_str_mv Neoplasias / patologia
Neoplasias / veterinária
Neoplasias da Mama / veterinária
Expressão Gênica / genética
Regulação da Expressão Gênica
topic Neoplasias / patologia
Neoplasias / veterinária
Neoplasias da Mama / veterinária
Expressão Gênica / genética
Regulação da Expressão Gênica
description Background/Aim: In female dogs, mammary cancer is the most frequent cancer type, accounting for 50% of all tumors affecting these animals. Amongst the commonly altered genes in cancer is the cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b), whose expression is negatively regulated by protein products of BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc) and T-box gene transcription factor 2 (Tbx2) genes. Considering this, the aim of this study was to evaluate the expression pattern of the Cdkn2b gene and these regulators in canine mammary tumors of dogs from Northern Brazil (Belém, Pará). Material and Methods: Gene expression in samples from 33 animals was assessed by real-time polymerase chain reaction. To check the influence of methylation on gene expression, bisulfite sequencing polymerase chain reaction was also performed. Results: All studied genes, except Cdkn2b, were found at increased expression levels in tumor tissue when compared with control samples. No correlation between expression and methylation data was observed. Conclusion: Our results suggest these markers may have a diagnostic value in the veterinary clinic.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-22T13:44:01Z
dc.date.available.fl_str_mv 2018-11-22T13:44:01Z
dc.date.issued.fl_str_mv 2018
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dc.identifier.citation.fl_str_mv FARO, Thamirys Aline Silva et al. Expression pattern of Cdkn2b and its regulators in canine mammary tumors. Anticancer Research, v. 38, n. 11, p. 6333-6338, Nov. 2018. DOI: https://doi.org/10.21873/anticanres.12991. Disponível em: https://ar.iiarjournals.org/content/anticanres/38/11/6333.full.pdf.
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dc.identifier.doi.pt_BR.fl_str_mv 10.21873/anticanres.12991.
identifier_str_mv FARO, Thamirys Aline Silva et al. Expression pattern of Cdkn2b and its regulators in canine mammary tumors. Anticancer Research, v. 38, n. 11, p. 6333-6338, Nov. 2018. DOI: https://doi.org/10.21873/anticanres.12991. Disponível em: https://ar.iiarjournals.org/content/anticanres/38/11/6333.full.pdf.
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