Deregulation of the SRC family tyrosine kinases in gastric arcinogenesis in Non-human Primates

• Autor(a) principal: Costa, Joana de Fátima Ferreira Borges da
• Data de Publicação: 2018
• Outros Autores: Sant'anna, Carla de Castro, Muniz, José Augusto Pereira Carneiro, Rocha, Carlos Alberto Machado da, Lamarão, Letícia Martins, Nunes, Caroline de Fátima Aquino Moreira, Assumpção, Paulo Pimentel de, Burbano, Rommel Mário Rodriguez
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4639; https://doi.org/10.21873/anticanres.12988
• topic: Primatas / anatomia & histologia; Neoplasias Intestinais / veterinária; Tirosina; Quinases da Família src; Carcinogênese / patologia

Background/aim: The evolution of gastric carcinogenesis remains largely unknown, as the regulatory mechanisms involved in the aggressiveness of gastric cancer are still poorly understood. Kinases are downstream modulators and effectors of various cell signaling cascades and play key roles in the development of neoplastic diseases. The objective of this study was to evaluate the expression of genes and proteins of the SRC family, including FYN, YES, BLK, FGR, LYN and SRC, in a model of intestinal gastric carcinogenesis generated by treating Cebus apella, a New World non-human primate, with N-methyl nitrosourea (MNU). Materials and methods: mRNA expression of genes was measured by real-time reverse transcription quantitative PCR (RT-qPCR) and protein expression was measured by western blotting in six Cebus apella treated with N-methyl-nitrosourea (MNU) for about 2.5 years. Results: Elevated mRNA and protein expression mainly of the SRC and LYN kinases was observed. Their expression was gradually increasing as non-atrophic gastritis was evolving to gastric cancer. Conclusion: SRC family kinases play a key role in tumor progression and metastasis and may be a promising target for the treatment of gastric cancer