Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842014000200489 |
Resumo: | Parkia pendula (Willd.) Walp. (Fabaceae) is a neotropical species of the genus Parkia more abundantly distributed in Central to South America. From the seeds of P. pendula a glucose/mannose specific lectin (PpeL) was isolated that has been characterised and used as a biotechnological tool but until now this is the first manuscript to analyse P. pendula mRNA expression in seedlings. For this porpoise a Differential display reverse transcription polimerase chain reaction (DDRT-PCR) was used to evaluate the expression of P. pendula lectin mRNAs in non-rooted seedlings. No bands were observed in the agarose gel, indicating the absence of mRNA of PpeL seedlings. our findings confirm that lectins mRNAs are differently regulated among species even if they are grouped in the same class. |
id |
IIE-1_8545eb0ec8195a44f9cec76af4998aac |
---|---|
oai_identifier_str |
oai:scielo:S1519-69842014000200489 |
network_acronym_str |
IIE-1 |
network_name_str |
Brazilian Journal of Biology |
repository_id_str |
|
spelling |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlingsPpeLDDRT-PCRseedlingsParkia pendula (Willd.) Walp. (Fabaceae) is a neotropical species of the genus Parkia more abundantly distributed in Central to South America. From the seeds of P. pendula a glucose/mannose specific lectin (PpeL) was isolated that has been characterised and used as a biotechnological tool but until now this is the first manuscript to analyse P. pendula mRNA expression in seedlings. For this porpoise a Differential display reverse transcription polimerase chain reaction (DDRT-PCR) was used to evaluate the expression of P. pendula lectin mRNAs in non-rooted seedlings. No bands were observed in the agarose gel, indicating the absence of mRNA of PpeL seedlings. our findings confirm that lectins mRNAs are differently regulated among species even if they are grouped in the same class.Instituto Internacional de Ecologia2014-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842014000200489Brazilian Journal of Biology v.74 n.2 2014reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.18512info:eu-repo/semantics/openAccessRêgo,MJBM.Santos,PB.Carvalho-Junior,LB.Stirling,J.Beltrão,EIC.eng2015-11-24T00:00:00Zoai:scielo:S1519-69842014000200489Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2015-11-24T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
title |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
spellingShingle |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings Rêgo,MJBM. PpeL DDRT-PCR seedlings |
title_short |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
title_full |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
title_fullStr |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
title_full_unstemmed |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
title_sort |
Evaluation of Parkia pendula lectin mRNA differentially expressed in seedlings |
author |
Rêgo,MJBM. |
author_facet |
Rêgo,MJBM. Santos,PB. Carvalho-Junior,LB. Stirling,J. Beltrão,EIC. |
author_role |
author |
author2 |
Santos,PB. Carvalho-Junior,LB. Stirling,J. Beltrão,EIC. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Rêgo,MJBM. Santos,PB. Carvalho-Junior,LB. Stirling,J. Beltrão,EIC. |
dc.subject.por.fl_str_mv |
PpeL DDRT-PCR seedlings |
topic |
PpeL DDRT-PCR seedlings |
description |
Parkia pendula (Willd.) Walp. (Fabaceae) is a neotropical species of the genus Parkia more abundantly distributed in Central to South America. From the seeds of P. pendula a glucose/mannose specific lectin (PpeL) was isolated that has been characterised and used as a biotechnological tool but until now this is the first manuscript to analyse P. pendula mRNA expression in seedlings. For this porpoise a Differential display reverse transcription polimerase chain reaction (DDRT-PCR) was used to evaluate the expression of P. pendula lectin mRNAs in non-rooted seedlings. No bands were observed in the agarose gel, indicating the absence of mRNA of PpeL seedlings. our findings confirm that lectins mRNAs are differently regulated among species even if they are grouped in the same class. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842014000200489 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842014000200489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1519-6984.18512 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.74 n.2 2014 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129880385912832 |