Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842019000200180 |
Resumo: | Abstract Synthetic polyploids are key breeding materials for watermelon. Compared with diploid watermelon, the tetraploid watermelon often exhibit wide phenotypic differences and differential gene expression. Digital gene expression (DGE) profile technique was performed in this study to present gene expression patterns in an autotetraploid and its progenitor diploid watermelon, and deferentially expressed genes (DEGs) related to the abiotic and biotic stress were also addressed. Altogether, 4,985 DEGs were obtained in the autotetraploid against its progenitor diploid, and 66.02% DEGs is up-regulated. GO analysis shows that these DEGs mainly distributed in ‘metabolic process’, ‘cell’ and ‘catalytic activity’. KEGG analysis revealed that these DEGs mainly cover ‘metabolic pathways’, ‘secondary metabolites’ and ‘ribosome’. Moreover, 134 tolerance related DEGs were identified which cover osmotic adjustment substance, protective enzymes/protein, signaling proteins and pathogenesis-related proteins. This study present the differential expression of stress related genes and global gene expression patterns at background level in autotetraploid watermelons. These new evidences could supplement the molecular theoretical basis for the better resistance after the genome doubling in the gourd family. |
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oai:scielo:S1519-69842019000200180 |
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Brazilian Journal of Biology |
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Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profilediploidyautotetraploidywatermelonDGE profileDEGsAbstract Synthetic polyploids are key breeding materials for watermelon. Compared with diploid watermelon, the tetraploid watermelon often exhibit wide phenotypic differences and differential gene expression. Digital gene expression (DGE) profile technique was performed in this study to present gene expression patterns in an autotetraploid and its progenitor diploid watermelon, and deferentially expressed genes (DEGs) related to the abiotic and biotic stress were also addressed. Altogether, 4,985 DEGs were obtained in the autotetraploid against its progenitor diploid, and 66.02% DEGs is up-regulated. GO analysis shows that these DEGs mainly distributed in ‘metabolic process’, ‘cell’ and ‘catalytic activity’. KEGG analysis revealed that these DEGs mainly cover ‘metabolic pathways’, ‘secondary metabolites’ and ‘ribosome’. Moreover, 134 tolerance related DEGs were identified which cover osmotic adjustment substance, protective enzymes/protein, signaling proteins and pathogenesis-related proteins. This study present the differential expression of stress related genes and global gene expression patterns at background level in autotetraploid watermelons. These new evidences could supplement the molecular theoretical basis for the better resistance after the genome doubling in the gourd family.Instituto Internacional de Ecologia2019-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842019000200180Brazilian Journal of Biology v.79 n.2 2019reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.174475info:eu-repo/semantics/openAccessLong,Y. L.Qiao,F.Jiang,X. F.Cong,H. Q.Sun,M. L.Xu,Z. J.eng2019-05-21T00:00:00Zoai:scielo:S1519-69842019000200180Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2019-05-21T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
title |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
spellingShingle |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile Long,Y. L. diploidy autotetraploidy watermelon DGE profile DEGs |
title_short |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
title_full |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
title_fullStr |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
title_full_unstemmed |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
title_sort |
Screening and analysis on the differentially expression genes between diploid and autotetraploid watermelon by using of digital gene expression profile |
author |
Long,Y. L. |
author_facet |
Long,Y. L. Qiao,F. Jiang,X. F. Cong,H. Q. Sun,M. L. Xu,Z. J. |
author_role |
author |
author2 |
Qiao,F. Jiang,X. F. Cong,H. Q. Sun,M. L. Xu,Z. J. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Long,Y. L. Qiao,F. Jiang,X. F. Cong,H. Q. Sun,M. L. Xu,Z. J. |
dc.subject.por.fl_str_mv |
diploidy autotetraploidy watermelon DGE profile DEGs |
topic |
diploidy autotetraploidy watermelon DGE profile DEGs |
description |
Abstract Synthetic polyploids are key breeding materials for watermelon. Compared with diploid watermelon, the tetraploid watermelon often exhibit wide phenotypic differences and differential gene expression. Digital gene expression (DGE) profile technique was performed in this study to present gene expression patterns in an autotetraploid and its progenitor diploid watermelon, and deferentially expressed genes (DEGs) related to the abiotic and biotic stress were also addressed. Altogether, 4,985 DEGs were obtained in the autotetraploid against its progenitor diploid, and 66.02% DEGs is up-regulated. GO analysis shows that these DEGs mainly distributed in ‘metabolic process’, ‘cell’ and ‘catalytic activity’. KEGG analysis revealed that these DEGs mainly cover ‘metabolic pathways’, ‘secondary metabolites’ and ‘ribosome’. Moreover, 134 tolerance related DEGs were identified which cover osmotic adjustment substance, protective enzymes/protein, signaling proteins and pathogenesis-related proteins. This study present the differential expression of stress related genes and global gene expression patterns at background level in autotetraploid watermelons. These new evidences could supplement the molecular theoretical basis for the better resistance after the genome doubling in the gourd family. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842019000200180 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842019000200180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1519-6984.174475 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.79 n.2 2019 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129885777690624 |