Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss

Detalhes bibliográficos
Autor(a) principal: Jan,T.
Data de Publicação: 2023
Outros Autores: Gul,S., Khan,A., Pervez,S., Noor,A., Amin,H., Bibi,S., Nawaz,M. A., Rahim,A., Ahmad,M. S., Azam,R., Ullah,H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100293
Resumo: Abstract Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days’ culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).
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spelling Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Biossagarculture vesselsgrowth regulatorsmacronutrientslamiaceaeAbstract Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days’ culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).Instituto Internacional de Ecologia2023-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100293Brazilian Journal of Biology v.83 2023reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.246904info:eu-repo/semantics/openAccessJan,T.Gul,S.Khan,A.Pervez,S.Noor,A.Amin,H.Bibi,S.Nawaz,M. A.Rahim,A.Ahmad,M. S.Azam,R.Ullah,H.eng2021-10-20T00:00:00Zoai:scielo:S1519-69842023000100293Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2021-10-20T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
title Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
spellingShingle Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
Jan,T.
agar
culture vessels
growth regulators
macronutrients
lamiaceae
title_short Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
title_full Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
title_fullStr Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
title_full_unstemmed Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
title_sort Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss
author Jan,T.
author_facet Jan,T.
Gul,S.
Khan,A.
Pervez,S.
Noor,A.
Amin,H.
Bibi,S.
Nawaz,M. A.
Rahim,A.
Ahmad,M. S.
Azam,R.
Ullah,H.
author_role author
author2 Gul,S.
Khan,A.
Pervez,S.
Noor,A.
Amin,H.
Bibi,S.
Nawaz,M. A.
Rahim,A.
Ahmad,M. S.
Azam,R.
Ullah,H.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jan,T.
Gul,S.
Khan,A.
Pervez,S.
Noor,A.
Amin,H.
Bibi,S.
Nawaz,M. A.
Rahim,A.
Ahmad,M. S.
Azam,R.
Ullah,H.
dc.subject.por.fl_str_mv agar
culture vessels
growth regulators
macronutrients
lamiaceae
topic agar
culture vessels
growth regulators
macronutrients
lamiaceae
description Abstract Hyperhydricity is a serious physiological disorder and affects In vitro propagation of many plants and as well of Salvia santolinifolia. The donor material to initiate the in vitro culture was the callus taken from the in vitro shoots produced on Murashig and Skoogs (MS) medium at 4.0 mg/l BA. This callus formed numerous hyperhydric shoots on culturing upon the medium of the same composition. The aim was to systematically evaluate the effect of cytokinins (Benzyladnine (BA) and N6-(-2-isopentenyl) adenine (2iP), culture vessels magnitude, medium solidification, source of nitrogen and calcium chloride for the alleviation of hyperhydricity. In the tissue cultures of S. santolinifolia BA and 2iP induced severe hyperhydricity, when other factors i.e. culture vessels magnitude and a suitable concentration of agar, ammonium nitrate (NH4NO3), potassium nitrate (KNO3) & calcium chloride (CaCl2.2H2O) were not optimized. After 30 days’ culture, we observed 83.82% hyperhydric shoots at increased level (1.5 mg/l 2iP) and 81.59% at decreased levels (1.0 mg/l 2iP). On the other hand, hyperhydricity percentage at decreased (0.4%) and at increased (0.8%) levels of agar were 72.37% and 39.08%, respectively. MS medium modification with NH4NO3 (412 mg/l), KNO3 (475 mg/l) and CaCl2.2H2O (880 mg/l) was found the best medium to reduced hyperhydricity (23.6%).
publishDate 2023
dc.date.none.fl_str_mv 2023-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100293
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100293
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1519-6984.246904
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Brazilian Journal of Biology v.83 2023
reponame:Brazilian Journal of Biology
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Brazilian Journal of Biology
collection Brazilian Journal of Biology
repository.name.fl_str_mv Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv bjb@bjb.com.br||bjb@bjb.com.br
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