Alternative method for quantification of alfa-amylase activity
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000200024 |
Resumo: | A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amylase from barley at the concentrations of 2.4; 60; 300 and 1,500 U.mL-1. The proposed method turned out to be simpler, faster, less expensive and able to determine on a macro-scale α-amylase over a wide range (2.4 to 7,500 U.mL-1) in scientific investigation as well as in teaching laboratory activities. |
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Brazilian Journal of Biology |
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Alternative method for quantification of alfa-amylase activityalfa-amylasealternative methodquantificationA modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amylase from barley at the concentrations of 2.4; 60; 300 and 1,500 U.mL-1. The proposed method turned out to be simpler, faster, less expensive and able to determine on a macro-scale α-amylase over a wide range (2.4 to 7,500 U.mL-1) in scientific investigation as well as in teaching laboratory activities.Instituto Internacional de Ecologia2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000200024Brazilian Journal of Biology v.70 n.2 2010reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S1519-69842010005000008info:eu-repo/semantics/openAccessFarias,DF.Carvalho,AFU.Oliveira,CC.Sousa,NM.Rocha-Bezerrra,LCB.Ferreira,PMP.Lima,GPG.Hissa,DC.eng2010-06-14T00:00:00Zoai:scielo:S1519-69842010000200024Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2010-06-14T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Alternative method for quantification of alfa-amylase activity |
title |
Alternative method for quantification of alfa-amylase activity |
spellingShingle |
Alternative method for quantification of alfa-amylase activity Farias,DF. alfa-amylase alternative method quantification |
title_short |
Alternative method for quantification of alfa-amylase activity |
title_full |
Alternative method for quantification of alfa-amylase activity |
title_fullStr |
Alternative method for quantification of alfa-amylase activity |
title_full_unstemmed |
Alternative method for quantification of alfa-amylase activity |
title_sort |
Alternative method for quantification of alfa-amylase activity |
author |
Farias,DF. |
author_facet |
Farias,DF. Carvalho,AFU. Oliveira,CC. Sousa,NM. Rocha-Bezerrra,LCB. Ferreira,PMP. Lima,GPG. Hissa,DC. |
author_role |
author |
author2 |
Carvalho,AFU. Oliveira,CC. Sousa,NM. Rocha-Bezerrra,LCB. Ferreira,PMP. Lima,GPG. Hissa,DC. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Farias,DF. Carvalho,AFU. Oliveira,CC. Sousa,NM. Rocha-Bezerrra,LCB. Ferreira,PMP. Lima,GPG. Hissa,DC. |
dc.subject.por.fl_str_mv |
alfa-amylase alternative method quantification |
topic |
alfa-amylase alternative method quantification |
description |
A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amylase from barley at the concentrations of 2.4; 60; 300 and 1,500 U.mL-1. The proposed method turned out to be simpler, faster, less expensive and able to determine on a macro-scale α-amylase over a wide range (2.4 to 7,500 U.mL-1) in scientific investigation as well as in teaching laboratory activities. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000200024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842010000200024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-69842010005000008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.70 n.2 2010 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129878750134272 |