Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

Detalhes bibliográficos
Autor(a) principal: Morais,Glaucia Almeida de
Data de Publicação: 1998
Outros Autores: Takaki,Massanori
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
Resumo: Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
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spelling Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. cariocaα-amylaseβ-amylasedeterminationselective inactivationPhaseolus vulgarisDetermination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.Instituto de Tecnologia do Paraná - Tecpar1998-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003Brazilian Archives of Biology and Technology v.41 n.1 1998reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89131998000100003info:eu-repo/semantics/openAccessMorais,Glaucia Almeida deTakaki,Massanorieng2011-09-21T00:00:00Zoai:scielo:S1516-89131998000100003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-09-21T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
spellingShingle Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Morais,Glaucia Almeida de
α-amylase
β-amylase
determination
selective inactivation
Phaseolus vulgaris
title_short Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_full Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_fullStr Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_full_unstemmed Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
title_sort Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
author Morais,Glaucia Almeida de
author_facet Morais,Glaucia Almeida de
Takaki,Massanori
author_role author
author2 Takaki,Massanori
author2_role author
dc.contributor.author.fl_str_mv Morais,Glaucia Almeida de
Takaki,Massanori
dc.subject.por.fl_str_mv α-amylase
β-amylase
determination
selective inactivation
Phaseolus vulgaris
topic α-amylase
β-amylase
determination
selective inactivation
Phaseolus vulgaris
description Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
publishDate 1998
dc.date.none.fl_str_mv 1998-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89131998000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.41 n.1 1998
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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