Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100105 |
Resumo: | Abstract High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment. |
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Brazilian Journal of Biology |
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spelling |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazildrug resistancesewagephenotypehospitalsAbstract High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment.Instituto Internacional de Ecologia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100105Brazilian Journal of Biology v.82 2022reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.234471info:eu-repo/semantics/openAccessZagui,G. S.Tonani,K. A. A.Fregonesi,B. M.Machado,G. P.Silva,T. V.Andrade,L. N.Andrade,D.Segura-Muñoz,S. I.eng2021-05-26T00:00:00Zoai:scielo:S1519-69842022000100105Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2021-05-26T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
title |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
spellingShingle |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil Zagui,G. S. drug resistance sewage phenotype hospitals |
title_short |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
title_full |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
title_fullStr |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
title_full_unstemmed |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
title_sort |
Tertiary hospital sewage as reservoir of bacteria expressing MDR phenotype in Brazil |
author |
Zagui,G. S. |
author_facet |
Zagui,G. S. Tonani,K. A. A. Fregonesi,B. M. Machado,G. P. Silva,T. V. Andrade,L. N. Andrade,D. Segura-Muñoz,S. I. |
author_role |
author |
author2 |
Tonani,K. A. A. Fregonesi,B. M. Machado,G. P. Silva,T. V. Andrade,L. N. Andrade,D. Segura-Muñoz,S. I. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Zagui,G. S. Tonani,K. A. A. Fregonesi,B. M. Machado,G. P. Silva,T. V. Andrade,L. N. Andrade,D. Segura-Muñoz,S. I. |
dc.subject.por.fl_str_mv |
drug resistance sewage phenotype hospitals |
topic |
drug resistance sewage phenotype hospitals |
description |
Abstract High doses of antibiotics used in hospitals can affect the microbial composition of sewers, selecting resistant bacteria. In this sense, we evaluated the antibiotic resistance profile and the multiresistant phenotype of bacteria isolated in sewage from a tertiary hospital in the interior São Paulo state, Brazil. For bacteria isolation, 10 µL of sewage samples were sown in selective culture media and the isolates were identified using VITEK-2 automatized system. The antibiotic sensitivity test was performed by disk diffusion. High percentages of resistance were found for amoxicillin, ampicillin, ceftazidime, clindamycin, vancomycin and the multidrug-resistant phenotype (MDR) was attributed to 60.7% of the isolates. Our results show bacteria classified as critical/high priority by WHO List of Priority Pathogens (Enterococcus and Staphylococcus aureus resistant to vancomycin and Enterobacteriaceae resistant to carbapenems) in hospital sewage. Therefore, the implementation of disinfection technologies for hospital sewage would reduce the bacterial load in the sewage that will reach urban wastewater treatment plants, minimizing superficial water contamination and bacterial resistance spread in the environment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100105 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1519-6984.234471 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Biology v.82 2022 reponame:Brazilian Journal of Biology instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Brazilian Journal of Biology |
collection |
Brazilian Journal of Biology |
repository.name.fl_str_mv |
Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
bjb@bjb.com.br||bjb@bjb.com.br |
_version_ |
1752129888393887744 |