Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Einstein (São Paulo) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082010000200187 |
Resumo: | ABSTRACT Objective: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. Methods: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. Results: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). Conclusions: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases. |
id |
IIEPAE-1_1e99a46aae3aeddb1fa932fe13bea5f5 |
---|---|
oai_identifier_str |
oai:scielo:S1679-45082010000200187 |
network_acronym_str |
IIEPAE-1 |
network_name_str |
Einstein (São Paulo) |
repository_id_str |
|
spelling |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samplesHomocysteineChromatography, high pressure liquidChemiluminescences measurementsABSTRACT Objective: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. Methods: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. Results: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). Conclusions: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.Instituto Israelita de Ensino e Pesquisa Albert Einstein2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082010000200187einstein (São Paulo) v.8 n.2 2010reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/s1679-45082010ao1499info:eu-repo/semantics/openAccessGascón,Thais MouraSchindler,FernandaOliveira,Claudia Giorgia Bronzatti deSouza,Fabiola Isabel SuanoHix,SoniaSarni,Roseli Oselka SacardoBacan,Ana Paula ArantesD'Almeida,VaniaGiglio,Auro delFonseca,Fernando Luiz Affonsoeng2017-03-14T00:00:00Zoai:scielo:S1679-45082010000200187Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2017-03-14T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false |
dc.title.none.fl_str_mv |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
title |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
spellingShingle |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples Gascón,Thais Moura Homocysteine Chromatography, high pressure liquid Chemiluminescences measurements |
title_short |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
title_full |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
title_fullStr |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
title_full_unstemmed |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
title_sort |
Evaluation of chemiluminescence method for the analysis of plasma homocysteine and comparison with HPLC method in children samples |
author |
Gascón,Thais Moura |
author_facet |
Gascón,Thais Moura Schindler,Fernanda Oliveira,Claudia Giorgia Bronzatti de Souza,Fabiola Isabel Suano Hix,Sonia Sarni,Roseli Oselka Sacardo Bacan,Ana Paula Arantes D'Almeida,Vania Giglio,Auro del Fonseca,Fernando Luiz Affonso |
author_role |
author |
author2 |
Schindler,Fernanda Oliveira,Claudia Giorgia Bronzatti de Souza,Fabiola Isabel Suano Hix,Sonia Sarni,Roseli Oselka Sacardo Bacan,Ana Paula Arantes D'Almeida,Vania Giglio,Auro del Fonseca,Fernando Luiz Affonso |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gascón,Thais Moura Schindler,Fernanda Oliveira,Claudia Giorgia Bronzatti de Souza,Fabiola Isabel Suano Hix,Sonia Sarni,Roseli Oselka Sacardo Bacan,Ana Paula Arantes D'Almeida,Vania Giglio,Auro del Fonseca,Fernando Luiz Affonso |
dc.subject.por.fl_str_mv |
Homocysteine Chromatography, high pressure liquid Chemiluminescences measurements |
topic |
Homocysteine Chromatography, high pressure liquid Chemiluminescences measurements |
description |
ABSTRACT Objective: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. Methods: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. Results: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). Conclusions: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082010000200187 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082010000200187 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1679-45082010ao1499 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
dc.source.none.fl_str_mv |
einstein (São Paulo) v.8 n.2 2010 reponame:Einstein (São Paulo) instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) instacron:IIEPAE |
instname_str |
Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
instacron_str |
IIEPAE |
institution |
IIEPAE |
reponame_str |
Einstein (São Paulo) |
collection |
Einstein (São Paulo) |
repository.name.fl_str_mv |
Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
repository.mail.fl_str_mv |
||revista@einstein.br |
_version_ |
1752129905805492224 |