Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Einstein (São Paulo) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100202 |
Resumo: | ABSTRACT Objective To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. Methods Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. Results Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. Conclusion Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects. |
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Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat dietBombyx moriObesity/drug therapySericins/therapeutic useLiverIntestine, smallJejunumDiet, high-fatMiceABSTRACT Objective To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. Methods Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. Results Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. Conclusion Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects.Instituto Israelita de Ensino e Pesquisa Albert Einstein2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100202einstein (São Paulo) v.18 2020reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.31744/einstein_journal/2020ao4876info:eu-repo/semantics/openAccessKunz,Regina InêsCapelassi,Angélica NoviAlegre-Maller,Ana Cláudia PaivaBonfleur,Maria LúciaRibeiro,Lucinéia de Fátima ChaskoCosta,Rose MeireNatali,Maria Raquel Marçaleng2019-09-23T00:00:00Zoai:scielo:S1679-45082020000100202Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2019-09-23T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false |
dc.title.none.fl_str_mv |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
title |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
spellingShingle |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet Kunz,Regina Inês Bombyx mori Obesity/drug therapy Sericins/therapeutic use Liver Intestine, small Jejunum Diet, high-fat Mice |
title_short |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
title_full |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
title_fullStr |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
title_full_unstemmed |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
title_sort |
Sericin as treatment of obesity: morphophysiological effects in obese mice fed with high-fat diet |
author |
Kunz,Regina Inês |
author_facet |
Kunz,Regina Inês Capelassi,Angélica Novi Alegre-Maller,Ana Cláudia Paiva Bonfleur,Maria Lúcia Ribeiro,Lucinéia de Fátima Chasko Costa,Rose Meire Natali,Maria Raquel Marçal |
author_role |
author |
author2 |
Capelassi,Angélica Novi Alegre-Maller,Ana Cláudia Paiva Bonfleur,Maria Lúcia Ribeiro,Lucinéia de Fátima Chasko Costa,Rose Meire Natali,Maria Raquel Marçal |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Kunz,Regina Inês Capelassi,Angélica Novi Alegre-Maller,Ana Cláudia Paiva Bonfleur,Maria Lúcia Ribeiro,Lucinéia de Fátima Chasko Costa,Rose Meire Natali,Maria Raquel Marçal |
dc.subject.por.fl_str_mv |
Bombyx mori Obesity/drug therapy Sericins/therapeutic use Liver Intestine, small Jejunum Diet, high-fat Mice |
topic |
Bombyx mori Obesity/drug therapy Sericins/therapeutic use Liver Intestine, small Jejunum Diet, high-fat Mice |
description |
ABSTRACT Objective To investigate the effects of sericin extracted from silkworm Bombyx mori cocoon on morphophysiological parameters in mice with obesity induced by high-fat diet. Methods Male C57Bl6 mice aged 9 weeks were allocated to one of two groups - Control and Obese, and fed a standard or high-fat diet for 10 weeks, respectively. Mice were then further subdivided into four groups with seven mice each, as follows: Control, Control-Sericin, Obese, and Obese-Sericin. The standard or high fat diet was given for 4 more weeks; sericin (1,000mg/kg body weight) was given orally to mice in the Control-Sericin and Obese-Sericin Groups during this period. Weight gain, food intake, fecal weight, fecal lipid content, gut motility and glucose tolerance were monitored. At the end of experimental period, plasma was collected for biochemical analysis. Samples of white adipose tissue, liver and jejunum were collected and processed for light microscopy analysis; liver fragments were used for lipid content determination. Results Obese mice experienced significantly greater weight gain and fat accumulation and had higher total cholesterol and glucose levels compared to controls. Retroperitoneal and periepididymal adipocyte hypertrophy, development of hepatic steatosis, increased cholesterol and triglyceride levels and morphometric changes in the jejunal wall were observed. Conclusion Physiological changes induced by obesity were not fully reverted by sericin; however, sericin treatment restored jejunal morphometry and increased lipid excretion in feces in obese mice, suggesting potential anti-obesity effects. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100202 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100202 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.31744/einstein_journal/2020ao4876 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
dc.source.none.fl_str_mv |
einstein (São Paulo) v.18 2020 reponame:Einstein (São Paulo) instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) instacron:IIEPAE |
instname_str |
Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
instacron_str |
IIEPAE |
institution |
IIEPAE |
reponame_str |
Einstein (São Paulo) |
collection |
Einstein (São Paulo) |
repository.name.fl_str_mv |
Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
repository.mail.fl_str_mv |
||revista@einstein.br |
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