Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Einstein (São Paulo) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013 |
Resumo: | OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis. |
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Isolation, cultivation and characterization of CD133+ stem cells from human glioblastomaGlioblastomaCell cultureNeoplastic stem cellsAntigensOBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.Instituto Israelita de Ensino e Pesquisa Albert Einstein2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013einstein (São Paulo) v.10 n.2 2012reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/S1679-45082012000200013info:eu-repo/semantics/openAccessPavon,Lorena FavaroMarti,Luciana CavalheiroSibov,Tatiana TaisMiyaki,Liza Aya MabuchiMalheiros,Suzana Maria FleuryMamani,Javier BustamanteBrandt,Reynaldo AndreRibas,Guilherme CarvalhalPagura,Jorge RobertoJoaquim,Marcos Augusto StavaleFeres Junior,HallinGamarra,Lionel Ferneleng2012-09-20T00:00:00Zoai:scielo:S1679-45082012000200013Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2012-09-20T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false |
dc.title.none.fl_str_mv |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
title |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
spellingShingle |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma Pavon,Lorena Favaro Glioblastoma Cell culture Neoplastic stem cells Antigens |
title_short |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
title_full |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
title_fullStr |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
title_full_unstemmed |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
title_sort |
Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma |
author |
Pavon,Lorena Favaro |
author_facet |
Pavon,Lorena Favaro Marti,Luciana Cavalheiro Sibov,Tatiana Tais Miyaki,Liza Aya Mabuchi Malheiros,Suzana Maria Fleury Mamani,Javier Bustamante Brandt,Reynaldo Andre Ribas,Guilherme Carvalhal Pagura,Jorge Roberto Joaquim,Marcos Augusto Stavale Feres Junior,Hallin Gamarra,Lionel Fernel |
author_role |
author |
author2 |
Marti,Luciana Cavalheiro Sibov,Tatiana Tais Miyaki,Liza Aya Mabuchi Malheiros,Suzana Maria Fleury Mamani,Javier Bustamante Brandt,Reynaldo Andre Ribas,Guilherme Carvalhal Pagura,Jorge Roberto Joaquim,Marcos Augusto Stavale Feres Junior,Hallin Gamarra,Lionel Fernel |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pavon,Lorena Favaro Marti,Luciana Cavalheiro Sibov,Tatiana Tais Miyaki,Liza Aya Mabuchi Malheiros,Suzana Maria Fleury Mamani,Javier Bustamante Brandt,Reynaldo Andre Ribas,Guilherme Carvalhal Pagura,Jorge Roberto Joaquim,Marcos Augusto Stavale Feres Junior,Hallin Gamarra,Lionel Fernel |
dc.subject.por.fl_str_mv |
Glioblastoma Cell culture Neoplastic stem cells Antigens |
topic |
Glioblastoma Cell culture Neoplastic stem cells Antigens |
description |
OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1679-45082012000200013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
dc.source.none.fl_str_mv |
einstein (São Paulo) v.10 n.2 2012 reponame:Einstein (São Paulo) instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) instacron:IIEPAE |
instname_str |
Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
instacron_str |
IIEPAE |
institution |
IIEPAE |
reponame_str |
Einstein (São Paulo) |
collection |
Einstein (São Paulo) |
repository.name.fl_str_mv |
Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
repository.mail.fl_str_mv |
||revista@einstein.br |
_version_ |
1752129906616041472 |