Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

Detalhes bibliográficos
Autor(a) principal: Pavon,Lorena Favaro
Data de Publicação: 2012
Outros Autores: Marti,Luciana Cavalheiro, Sibov,Tatiana Tais, Miyaki,Liza Aya Mabuchi, Malheiros,Suzana Maria Fleury, Mamani,Javier Bustamante, Brandt,Reynaldo Andre, Ribas,Guilherme Carvalhal, Pagura,Jorge Roberto, Joaquim,Marcos Augusto Stavale, Feres Junior,Hallin, Gamarra,Lionel Fernel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Einstein (São Paulo)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013
Resumo: OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.
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spelling Isolation, cultivation and characterization of CD133+ stem cells from human glioblastomaGlioblastomaCell cultureNeoplastic stem cellsAntigensOBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.Instituto Israelita de Ensino e Pesquisa Albert Einstein2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013einstein (São Paulo) v.10 n.2 2012reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/S1679-45082012000200013info:eu-repo/semantics/openAccessPavon,Lorena FavaroMarti,Luciana CavalheiroSibov,Tatiana TaisMiyaki,Liza Aya MabuchiMalheiros,Suzana Maria FleuryMamani,Javier BustamanteBrandt,Reynaldo AndreRibas,Guilherme CarvalhalPagura,Jorge RobertoJoaquim,Marcos Augusto StavaleFeres Junior,HallinGamarra,Lionel Ferneleng2012-09-20T00:00:00Zoai:scielo:S1679-45082012000200013Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2012-09-20T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false
dc.title.none.fl_str_mv Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
title Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
spellingShingle Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
Pavon,Lorena Favaro
Glioblastoma
Cell culture
Neoplastic stem cells
Antigens
title_short Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
title_full Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
title_fullStr Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
title_full_unstemmed Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
title_sort Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
author Pavon,Lorena Favaro
author_facet Pavon,Lorena Favaro
Marti,Luciana Cavalheiro
Sibov,Tatiana Tais
Miyaki,Liza Aya Mabuchi
Malheiros,Suzana Maria Fleury
Mamani,Javier Bustamante
Brandt,Reynaldo Andre
Ribas,Guilherme Carvalhal
Pagura,Jorge Roberto
Joaquim,Marcos Augusto Stavale
Feres Junior,Hallin
Gamarra,Lionel Fernel
author_role author
author2 Marti,Luciana Cavalheiro
Sibov,Tatiana Tais
Miyaki,Liza Aya Mabuchi
Malheiros,Suzana Maria Fleury
Mamani,Javier Bustamante
Brandt,Reynaldo Andre
Ribas,Guilherme Carvalhal
Pagura,Jorge Roberto
Joaquim,Marcos Augusto Stavale
Feres Junior,Hallin
Gamarra,Lionel Fernel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pavon,Lorena Favaro
Marti,Luciana Cavalheiro
Sibov,Tatiana Tais
Miyaki,Liza Aya Mabuchi
Malheiros,Suzana Maria Fleury
Mamani,Javier Bustamante
Brandt,Reynaldo Andre
Ribas,Guilherme Carvalhal
Pagura,Jorge Roberto
Joaquim,Marcos Augusto Stavale
Feres Junior,Hallin
Gamarra,Lionel Fernel
dc.subject.por.fl_str_mv Glioblastoma
Cell culture
Neoplastic stem cells
Antigens
topic Glioblastoma
Cell culture
Neoplastic stem cells
Antigens
description OBJECTIVE: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. METHODS: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133+) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. RESULTS: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. CONCLUSION: These findings may contribute to a better understanding of the process of gliomagenesis.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000200013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1679-45082012000200013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Israelita de Ensino e Pesquisa Albert Einstein
publisher.none.fl_str_mv Instituto Israelita de Ensino e Pesquisa Albert Einstein
dc.source.none.fl_str_mv einstein (São Paulo) v.10 n.2 2012
reponame:Einstein (São Paulo)
instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
instacron:IIEPAE
instname_str Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
instacron_str IIEPAE
institution IIEPAE
reponame_str Einstein (São Paulo)
collection Einstein (São Paulo)
repository.name.fl_str_mv Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
repository.mail.fl_str_mv ||revista@einstein.br
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