SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Einstein (São Paulo) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082014000200191 |
Resumo: | Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only to LightCycler™ SeptiFast and one to different pathogens found by each test. Infections with microorganisms detected only using blood culture reassured the need to perform both tests. The mean time to results for blood culture was 5 days for negative and 3.5 days for positive results. LightCycler™ SeptiFast results were achieved in less than 8 hours.Conclusion LightCycler™ SeptiFast showed a high potential as a test to be carried out concomitantly with blood culture for sepsis diagnosis in severely ill patients. This test allowed a faster diagnosis of bacterial and fungal infections that helped to reduce hospital stay and to control the use of antibiotics. LightCycler™ SeptiFast can also eventually detect microorganism and infections that are hardly detected by blood culture, especiallyCandidanon-albicans infections. |
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SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospitalSepsis/diagnosisMultiplex polymerase chain reactionReal-time polymerase chain reactionObjective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only to LightCycler™ SeptiFast and one to different pathogens found by each test. Infections with microorganisms detected only using blood culture reassured the need to perform both tests. The mean time to results for blood culture was 5 days for negative and 3.5 days for positive results. LightCycler™ SeptiFast results were achieved in less than 8 hours.Conclusion LightCycler™ SeptiFast showed a high potential as a test to be carried out concomitantly with blood culture for sepsis diagnosis in severely ill patients. This test allowed a faster diagnosis of bacterial and fungal infections that helped to reduce hospital stay and to control the use of antibiotics. LightCycler™ SeptiFast can also eventually detect microorganism and infections that are hardly detected by blood culture, especiallyCandidanon-albicans infections.Instituto Israelita de Ensino e Pesquisa Albert Einstein2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082014000200191einstein (São Paulo) v.12 n.2 2014reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/S1679-45082014AO2932info:eu-repo/semantics/openAccessSitnik,RobertaMarra,Alexandre RodriguesPetroni,Roberta CardosoRamos,Ozires Pereira SantosMartino,Marinês Dalla VallePasternak,JacyrSantos,Oscar Fernando Pavão dosMangueira,Cristóvão Luis PitangueiraPinho,João Renato Rebelloeng2015-09-10T00:00:00Zoai:scielo:S1679-45082014000200191Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2015-09-10T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false |
dc.title.none.fl_str_mv |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
title |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
spellingShingle |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital Sitnik,Roberta Sepsis/diagnosis Multiplex polymerase chain reaction Real-time polymerase chain reaction |
title_short |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
title_full |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
title_fullStr |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
title_full_unstemmed |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
title_sort |
SeptiFast for diagnosis of sepsis in severely ill patients from a Brazilian hospital |
author |
Sitnik,Roberta |
author_facet |
Sitnik,Roberta Marra,Alexandre Rodrigues Petroni,Roberta Cardoso Ramos,Ozires Pereira Santos Martino,Marinês Dalla Valle Pasternak,Jacyr Santos,Oscar Fernando Pavão dos Mangueira,Cristóvão Luis Pitangueira Pinho,João Renato Rebello |
author_role |
author |
author2 |
Marra,Alexandre Rodrigues Petroni,Roberta Cardoso Ramos,Ozires Pereira Santos Martino,Marinês Dalla Valle Pasternak,Jacyr Santos,Oscar Fernando Pavão dos Mangueira,Cristóvão Luis Pitangueira Pinho,João Renato Rebello |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sitnik,Roberta Marra,Alexandre Rodrigues Petroni,Roberta Cardoso Ramos,Ozires Pereira Santos Martino,Marinês Dalla Valle Pasternak,Jacyr Santos,Oscar Fernando Pavão dos Mangueira,Cristóvão Luis Pitangueira Pinho,João Renato Rebello |
dc.subject.por.fl_str_mv |
Sepsis/diagnosis Multiplex polymerase chain reaction Real-time polymerase chain reaction |
topic |
Sepsis/diagnosis Multiplex polymerase chain reaction Real-time polymerase chain reaction |
description |
Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well as to compare the results with conventional blood culture.Methods A total of 114 consecutive patients with clinical evidence of sepsis were submitted to blood culture and LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) were detected using the LightCycler™ SeptiFast than the blood culture (17; 14.9%), with an agreement of 86.8%. Discordant results were seen in four patients positive only to blood culture, ten positive only to LightCycler™ SeptiFast and one to different pathogens found by each test. Infections with microorganisms detected only using blood culture reassured the need to perform both tests. The mean time to results for blood culture was 5 days for negative and 3.5 days for positive results. LightCycler™ SeptiFast results were achieved in less than 8 hours.Conclusion LightCycler™ SeptiFast showed a high potential as a test to be carried out concomitantly with blood culture for sepsis diagnosis in severely ill patients. This test allowed a faster diagnosis of bacterial and fungal infections that helped to reduce hospital stay and to control the use of antibiotics. LightCycler™ SeptiFast can also eventually detect microorganism and infections that are hardly detected by blood culture, especiallyCandidanon-albicans infections. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082014000200191 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082014000200191 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1679-45082014AO2932 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
dc.source.none.fl_str_mv |
einstein (São Paulo) v.12 n.2 2014 reponame:Einstein (São Paulo) instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) instacron:IIEPAE |
instname_str |
Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
instacron_str |
IIEPAE |
institution |
IIEPAE |
reponame_str |
Einstein (São Paulo) |
collection |
Einstein (São Paulo) |
repository.name.fl_str_mv |
Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
repository.mail.fl_str_mv |
||revista@einstein.br |
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1752129907440222208 |