In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
Autor(a) principal: | |
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Data de Publicação: | 1980 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Hansenologia Internationalis (Online) |
Texto Completo: | https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105 |
Resumo: | A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed. |
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In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformationDiamino-difenil-sulfonaFitohemaglutininaTransformação de linfócitosDiamino diphenyl sulphonePhytohaemagglutininLymphocyte transformation testA study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.Estudou-se o efeito do DDS. sobre a transformação linfocitária estimulada pela PHA, in vitro, utilizando-se concentrações variáveis de DDS. Os linfócitos foram doados por voluntários, divididos em três grupos, e o estudo foi desenvolvido em três fases. Foram empregadas 4 concentrações diferentes de DDS para 106 de linfócitos em cultura de tecidos além de 0,02 ml de PHA. Foi observada uma diminuição estatisticamente significativa (P<0,05) no percentual de formação de células blásticas induzida pela PHA com todas as concentrações de DDS, exceto com a concentração mais baixa (0,01 eg de DDS). Enquanto que a diminuição observada na primeira e na terceira fases mostrou-se dependente da dose de DDS, na segunda fase não se encontrou correlação significativa entre a concentração de DDS e a depressão da blastogênese induzida pela PHA. Discute-se a significância destas observações.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1980-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3610510.47878/hi.1980.v5.36105Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 2 (1980); 112-118Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 2 (1980); 112-1181982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLenghttps://periodicos.saude.sp.gov.br/hansenologia/article/view/36105/34415https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGhei, S.K.Sengupta, U.Desikan, K.V.2023-11-29T14:01:34Zoai:ojs.periodicos.saude.sp.gov.br:article/36105Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2023-11-29T14:01:34Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false |
dc.title.none.fl_str_mv |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
title |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
spellingShingle |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation Ghei, S.K. Diamino-difenil-sulfona Fitohemaglutinina Transformação de linfócitos Diamino diphenyl sulphone Phytohaemagglutinin Lymphocyte transformation test |
title_short |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
title_full |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
title_fullStr |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
title_full_unstemmed |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
title_sort |
In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation |
author |
Ghei, S.K. |
author_facet |
Ghei, S.K. Sengupta, U. Desikan, K.V. |
author_role |
author |
author2 |
Sengupta, U. Desikan, K.V. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ghei, S.K. Sengupta, U. Desikan, K.V. |
dc.subject.por.fl_str_mv |
Diamino-difenil-sulfona Fitohemaglutinina Transformação de linfócitos Diamino diphenyl sulphone Phytohaemagglutinin Lymphocyte transformation test |
topic |
Diamino-difenil-sulfona Fitohemaglutinina Transformação de linfócitos Diamino diphenyl sulphone Phytohaemagglutinin Lymphocyte transformation test |
description |
A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed. |
publishDate |
1980 |
dc.date.none.fl_str_mv |
1980-11-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105 10.47878/hi.1980.v5.36105 |
url |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105 |
identifier_str_mv |
10.47878/hi.1980.v5.36105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105/34415 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo |
publisher.none.fl_str_mv |
Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo |
dc.source.none.fl_str_mv |
Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 2 (1980); 112-118 Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 2 (1980); 112-118 1982-5161 reponame:Hansenologia Internationalis (Online) instname:Instituto Lauro de Souza Lima (ILSL) instacron:ILSL |
instname_str |
Instituto Lauro de Souza Lima (ILSL) |
instacron_str |
ILSL |
institution |
ILSL |
reponame_str |
Hansenologia Internationalis (Online) |
collection |
Hansenologia Internationalis (Online) |
repository.name.fl_str_mv |
Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL) |
repository.mail.fl_str_mv |
hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br |
_version_ |
1796797580532776960 |