In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation

Detalhes bibliográficos
Autor(a) principal: Ghei, S.K.
Data de Publicação: 1980
Outros Autores: Sengupta, U., Desikan, K.V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hansenologia Internationalis (Online)
Texto Completo: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105
Resumo: A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.
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spelling In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformationDiamino-difenil-sulfonaFitohemaglutininaTransformação de linfócitosDiamino diphenyl sulphonePhytohaemagglutininLymphocyte transformation testA study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.Estudou-se o efeito do DDS. sobre a transformação linfocitária estimulada pela PHA, in vitro, utilizando-se concentrações variáveis de DDS. Os linfócitos foram doados por voluntários, divididos em três grupos, e o estudo foi desenvolvido em três fases. Foram empregadas 4 concentrações diferentes de DDS para 106 de linfócitos em cultura de tecidos além de 0,02 ml de PHA. Foi observada uma diminuição estatisticamente significativa (P<0,05) no percentual de formação de células blásticas induzida pela PHA com todas as concentrações de DDS, exceto com a concentração mais baixa (0,01 eg de DDS). Enquanto que a diminuição observada na primeira e na terceira fases mostrou-se dependente da dose de DDS, na segunda fase não se encontrou correlação significativa entre a concentração de DDS e a depressão da blastogênese induzida pela PHA. Discute-se a significância destas observações.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1980-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3610510.47878/hi.1980.v5.36105Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 2 (1980); 112-118Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 2 (1980); 112-1181982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLenghttps://periodicos.saude.sp.gov.br/hansenologia/article/view/36105/34415https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGhei, S.K.Sengupta, U.Desikan, K.V.2023-11-29T14:01:34Zoai:ojs.periodicos.saude.sp.gov.br:article/36105Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2023-11-29T14:01:34Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false
dc.title.none.fl_str_mv In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
title In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
spellingShingle In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
Ghei, S.K.
Diamino-difenil-sulfona
Fitohemaglutinina
Transformação de linfócitos
Diamino diphenyl sulphone
Phytohaemagglutinin
Lymphocyte transformation test
title_short In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
title_full In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
title_fullStr In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
title_full_unstemmed In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
title_sort In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation
author Ghei, S.K.
author_facet Ghei, S.K.
Sengupta, U.
Desikan, K.V.
author_role author
author2 Sengupta, U.
Desikan, K.V.
author2_role author
author
dc.contributor.author.fl_str_mv Ghei, S.K.
Sengupta, U.
Desikan, K.V.
dc.subject.por.fl_str_mv Diamino-difenil-sulfona
Fitohemaglutinina
Transformação de linfócitos
Diamino diphenyl sulphone
Phytohaemagglutinin
Lymphocyte transformation test
topic Diamino-difenil-sulfona
Fitohemaglutinina
Transformação de linfócitos
Diamino diphenyl sulphone
Phytohaemagglutinin
Lymphocyte transformation test
description A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.
publishDate 1980
dc.date.none.fl_str_mv 1980-11-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105
10.47878/hi.1980.v5.36105
url https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105
identifier_str_mv 10.47878/hi.1980.v5.36105
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/36105/34415
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
dc.source.none.fl_str_mv Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 2 (1980); 112-118
Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 2 (1980); 112-118
1982-5161
reponame:Hansenologia Internationalis (Online)
instname:Instituto Lauro de Souza Lima (ILSL)
instacron:ILSL
instname_str Instituto Lauro de Souza Lima (ILSL)
instacron_str ILSL
institution ILSL
reponame_str Hansenologia Internationalis (Online)
collection Hansenologia Internationalis (Online)
repository.name.fl_str_mv Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)
repository.mail.fl_str_mv hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br
_version_ 1796797580532776960

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