MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/78678 |
Resumo: | SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans. |
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MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS Tipagem molecular de Candida albicans isoladas de pacientes hospitalizados SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans. RESUMO Introdução: A maioria das infecções fúngicas hospitalares são causadas por Candida spp. e C. albicans é a espécie mais comumente identificada. Métodos moleculares são ferramentas importantes para a avaliação da origem das leveduras isoladas em hospitais. Métodos: Este é um estudo sobre o perfil genético de 39 isolados clínicos nosocomiais de C. albicans através das técnicas de RAPD e microssatélite, foram usados dois diferentes iniciadores para cada técnica. Resultados: RAPD forneceu 10 e 11 diferentes perfis com valores de SAB 0,84 ± 0,126 e 0,88 ± 0,08 para os primers M2 e P4, respectivamente. A análise de microssatélites, usando os marcadores CDC3 e HIS3 permitiu a observação de seis e sete diferentes alelos respectivamente, com poder discriminatório combinado de 0,91. Conclusões: Embora seja clara a variabilidade genética, foi possível identificar alta similaridade, sugerindo origem comum para pelo menos parte deles. É importante enfatizar que foi comprovada origem comum de leveduras isoladas de colonização (urina, cateter ou secreção orotraqueal) e hemocultura do mesmo paciente, indicando que a candidemia deve ter iniciado a partir de um sítio de colonização. A combinação das técnicas RAPD e microssatélites fornece uma análise rápida e eficiente para investigação de similaridade entre isolados nosocomiais de C. albicans. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/78678Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 6 (2013); 385-391Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 6 (2013); 385-391Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 6 (2013); 385-3911678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/78678/82730Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBonfim-Mendonca, Patricia de Souza Fiorini, Adriana Shinobu-Mesquita, Cristiane Suemi Baeza, Lilian Cristiane Fernandez, Maria Aparecida Svidzinski, Terezinha Inez Estivalet 2014-04-03T18:01:14Zoai:revistas.usp.br:article/78678Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:15.055204Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS Tipagem molecular de Candida albicans isoladas de pacientes hospitalizados |
title |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
spellingShingle |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS Bonfim-Mendonca, Patricia de Souza |
title_short |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
title_full |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
title_fullStr |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
title_full_unstemmed |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
title_sort |
MOLECULAR TYPING OF Candida albicans ISOLATES FROM HOSPITALIZED PATIENTS |
author |
Bonfim-Mendonca, Patricia de Souza |
author_facet |
Bonfim-Mendonca, Patricia de Souza Fiorini, Adriana Shinobu-Mesquita, Cristiane Suemi Baeza, Lilian Cristiane Fernandez, Maria Aparecida Svidzinski, Terezinha Inez Estivalet |
author_role |
author |
author2 |
Fiorini, Adriana Shinobu-Mesquita, Cristiane Suemi Baeza, Lilian Cristiane Fernandez, Maria Aparecida Svidzinski, Terezinha Inez Estivalet |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bonfim-Mendonca, Patricia de Souza Fiorini, Adriana Shinobu-Mesquita, Cristiane Suemi Baeza, Lilian Cristiane Fernandez, Maria Aparecida Svidzinski, Terezinha Inez Estivalet |
description |
SUMMARY Introduction: The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals. Methods: This is a study on the genetic profifiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplifified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used. Results: RAPD provided 10 and 11 different profiles with values for SAB of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory power of 0.91. Conclusions: Although genetic variability is clear, it was possible to identify high similarity, suggesting a common origin for at least a part of isolates. It is important to emphasize that common origin was proven from yeasts isolated from colonization (urine, catheter or endotracheal secretions) and blood culture from the same patient, indicating that the candidemia must have started from a site of colonization. The combination of RAPD and microsatellite provides a quick and efficient analysis for investigation of similarity among nosocomial isolates of C. albicans. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/78678 |
url |
https://www.revistas.usp.br/rimtsp/article/view/78678 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/78678/82730 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 6 (2013); 385-391 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 6 (2013); 385-391 Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 6 (2013); 385-391 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
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