Molecular typing of IberoAmerican Cryptococcus neoformans isolates
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm http://hdl.handle.net/11449/67195 |
Resumo: | A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates. |
id |
UNSP_fe3adadea4dac66597e16e26aebc90bf |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/67195 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Molecular typing of IberoAmerican Cryptococcus neoformans isolatescontrolled studyCryptococcus neoformansDNA fingerprintingDNA polymorphismfungal genefungus isolationgenetic analysisgeographic distributionmolecular typingnonhumanpolymerase chain reactionrestriction fragment length polymorphismserotypeura5 genevirus geneAdultAgedAnimalsBacterial Typing TechniquesCentral AmericaCryptococcosisDNA FingerprintingEpidemiology, MolecularFemaleGoatsHumansOrotate PhosphoribosyltransferaseParrotsPhylogenyPolymorphism, GeneticPolymorphism, Restriction Fragment LengthSouth AmericaSpainA network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.University of Sydney, Sydney, NSWInstituto Nacional de Salud, BogotaUniversity of Western Sydney, Campbelltown, NSWMolecular Mycology Laboratory CIDM Westmead Hospital, Darcy Road, Westmead, NSW 2145Hosp. de Infecc. Francisco J. Muniz, Buenos AiresDepartamento Micologia Inst. Nac. Enferm. I. Dr. C.G.M., Buenos AiresLaboratorio de Micologia Medica Hospital Evandro Chagas FundaVao Oswaldo Cruz, Rio de JaneiroFaculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), AraraquaraAdolfo Lutz Inst. SeVao de Micologia, São PauloCentro de Biotechnologia (UFRGS), Porto AlegreProg. de Microbiologia y Micologia Universidad de Chile, SantiagoCorp. para Invest. Biologicas, MedellinInstituto Nacional de Salud, BogotáHospital San Juan de Dios, Guatemala CityUniversidad de San Carlos, Guatemala CityDept. de Microbiologia/Parasitologia Facultad de Medicina Univ. Nacional Autonoma de Mexico, México CityInst. Nac. de Diagn./Ref. Epidemiol., Mexico CityInst. de Med. Trop. A. Humboldt, LimaGrup Recerca en Micologia Exp./Clin. Inst. Munic. d'Investigacio Medica Universitat Autonoma de Barcelona, BarcelonaUniversidad del Zulia, MaracaiboFaculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), AraraquaraUniversity of SydneyInstituto Nacional de SaludUniversity of Western SydneyWestmead HospitalHosp. de Infecc. Francisco J. MunizInst. Nac. Enferm. I. Dr. C.G.M.FundaVao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade Federal do Rio Grande do Sul (UFRGS)Universidad de ChileCorp. para Invest. BiologicasHospital San Juan de DiosUniversidad de San CarlosUniv. Nacional Autonoma de MexicoInst. Nac. de Diagn./Ref. Epidemiol.Inst. de Med. Trop. A. HumboldtUniversitat Autonoma de BarcelonaUniversidad del ZuliaMeyer, WielandCastañeda, AlexandraJackson, StuartHuynh, MatthewCastañeda, ElizabethArechavala, AliciaDavel, GracielaRodero, LauraPerrotta, DiegoLazera, MarciaPereira-Igreja, RicardoWanke, BodoMendes-Giannini, Maria José Soares [UNESP]Melhem, Marcia S.C.Henning-Vainstein, MarleneDiaz, Maria CristinaRestrepo, AngelaHuérfano, SandraSamayoa, BlancaLogeman, HeidiMartirez, Rubén LópezOlivares, Laura Rocio CastañonContreras-Peres, CuadbertoTovar, José Francisco ValenzuelaBustamante, BeatrizTorres-Rodriquez, JosephMorera, YolandaCalvo, Belinda2014-05-27T11:20:36Z2014-05-27T11:20:36Z2003-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article189-195application/pdfhttp://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htmEmerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003.1080-6040http://hdl.handle.net/11449/671952-s2.0-03450741172-s2.0-0345074117.pdf0000-0002-8059-0826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEmerging Infectious Diseases7.4223,278info:eu-repo/semantics/openAccess2024-06-21T15:18:56Zoai:repositorio.unesp.br:11449/67195Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:24:09.949971Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
title |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
spellingShingle |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates Meyer, Wieland controlled study Cryptococcus neoformans DNA fingerprinting DNA polymorphism fungal gene fungus isolation genetic analysis geographic distribution molecular typing nonhuman polymerase chain reaction restriction fragment length polymorphism serotype ura5 gene virus gene Adult Aged Animals Bacterial Typing Techniques Central America Cryptococcosis DNA Fingerprinting Epidemiology, Molecular Female Goats Humans Orotate Phosphoribosyltransferase Parrots Phylogeny Polymorphism, Genetic Polymorphism, Restriction Fragment Length South America Spain |
title_short |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
title_full |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
title_fullStr |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
title_full_unstemmed |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
title_sort |
Molecular typing of IberoAmerican Cryptococcus neoformans isolates |
author |
Meyer, Wieland |
author_facet |
Meyer, Wieland Castañeda, Alexandra Jackson, Stuart Huynh, Matthew Castañeda, Elizabeth Arechavala, Alicia Davel, Graciela Rodero, Laura Perrotta, Diego Lazera, Marcia Pereira-Igreja, Ricardo Wanke, Bodo Mendes-Giannini, Maria José Soares [UNESP] Melhem, Marcia S.C. Henning-Vainstein, Marlene Diaz, Maria Cristina Restrepo, Angela Huérfano, Sandra Samayoa, Blanca Logeman, Heidi Martirez, Rubén López Olivares, Laura Rocio Castañon Contreras-Peres, Cuadberto Tovar, José Francisco Valenzuela Bustamante, Beatriz Torres-Rodriquez, Joseph Morera, Yolanda Calvo, Belinda |
author_role |
author |
author2 |
Castañeda, Alexandra Jackson, Stuart Huynh, Matthew Castañeda, Elizabeth Arechavala, Alicia Davel, Graciela Rodero, Laura Perrotta, Diego Lazera, Marcia Pereira-Igreja, Ricardo Wanke, Bodo Mendes-Giannini, Maria José Soares [UNESP] Melhem, Marcia S.C. Henning-Vainstein, Marlene Diaz, Maria Cristina Restrepo, Angela Huérfano, Sandra Samayoa, Blanca Logeman, Heidi Martirez, Rubén López Olivares, Laura Rocio Castañon Contreras-Peres, Cuadberto Tovar, José Francisco Valenzuela Bustamante, Beatriz Torres-Rodriquez, Joseph Morera, Yolanda Calvo, Belinda |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Sydney Instituto Nacional de Salud University of Western Sydney Westmead Hospital Hosp. de Infecc. Francisco J. Muniz Inst. Nac. Enferm. I. Dr. C.G.M. FundaVao Oswaldo Cruz Universidade Estadual Paulista (Unesp) Instituto Adolfo Lutz (IAL) Universidade Federal do Rio Grande do Sul (UFRGS) Universidad de Chile Corp. para Invest. Biologicas Hospital San Juan de Dios Universidad de San Carlos Univ. Nacional Autonoma de Mexico Inst. Nac. de Diagn./Ref. Epidemiol. Inst. de Med. Trop. A. Humboldt Universitat Autonoma de Barcelona Universidad del Zulia |
dc.contributor.author.fl_str_mv |
Meyer, Wieland Castañeda, Alexandra Jackson, Stuart Huynh, Matthew Castañeda, Elizabeth Arechavala, Alicia Davel, Graciela Rodero, Laura Perrotta, Diego Lazera, Marcia Pereira-Igreja, Ricardo Wanke, Bodo Mendes-Giannini, Maria José Soares [UNESP] Melhem, Marcia S.C. Henning-Vainstein, Marlene Diaz, Maria Cristina Restrepo, Angela Huérfano, Sandra Samayoa, Blanca Logeman, Heidi Martirez, Rubén López Olivares, Laura Rocio Castañon Contreras-Peres, Cuadberto Tovar, José Francisco Valenzuela Bustamante, Beatriz Torres-Rodriquez, Joseph Morera, Yolanda Calvo, Belinda |
dc.subject.por.fl_str_mv |
controlled study Cryptococcus neoformans DNA fingerprinting DNA polymorphism fungal gene fungus isolation genetic analysis geographic distribution molecular typing nonhuman polymerase chain reaction restriction fragment length polymorphism serotype ura5 gene virus gene Adult Aged Animals Bacterial Typing Techniques Central America Cryptococcosis DNA Fingerprinting Epidemiology, Molecular Female Goats Humans Orotate Phosphoribosyltransferase Parrots Phylogeny Polymorphism, Genetic Polymorphism, Restriction Fragment Length South America Spain |
topic |
controlled study Cryptococcus neoformans DNA fingerprinting DNA polymorphism fungal gene fungus isolation genetic analysis geographic distribution molecular typing nonhuman polymerase chain reaction restriction fragment length polymorphism serotype ura5 gene virus gene Adult Aged Animals Bacterial Typing Techniques Central America Cryptococcosis DNA Fingerprinting Epidemiology, Molecular Female Goats Humans Orotate Phosphoribosyltransferase Parrots Phylogeny Polymorphism, Genetic Polymorphism, Restriction Fragment Length South America Spain |
description |
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-02-01 2014-05-27T11:20:36Z 2014-05-27T11:20:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm Emerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003. 1080-6040 http://hdl.handle.net/11449/67195 2-s2.0-0345074117 2-s2.0-0345074117.pdf 0000-0002-8059-0826 |
url |
http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm http://hdl.handle.net/11449/67195 |
identifier_str_mv |
Emerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003. 1080-6040 2-s2.0-0345074117 2-s2.0-0345074117.pdf 0000-0002-8059-0826 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Emerging Infectious Diseases 7.422 3,278 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
189-195 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128928010731520 |