Molecular typing of IberoAmerican Cryptococcus neoformans isolates

Detalhes bibliográficos
Autor(a) principal: Meyer, Wieland
Data de Publicação: 2003
Outros Autores: Castañeda, Alexandra, Jackson, Stuart, Huynh, Matthew, Castañeda, Elizabeth, Arechavala, Alicia, Davel, Graciela, Rodero, Laura, Perrotta, Diego, Lazera, Marcia, Pereira-Igreja, Ricardo, Wanke, Bodo, Mendes-Giannini, Maria José Soares [UNESP], Melhem, Marcia S.C., Henning-Vainstein, Marlene, Diaz, Maria Cristina, Restrepo, Angela, Huérfano, Sandra, Samayoa, Blanca, Logeman, Heidi, Martirez, Rubén López, Olivares, Laura Rocio Castañon, Contreras-Peres, Cuadberto, Tovar, José Francisco Valenzuela, Bustamante, Beatriz, Torres-Rodriquez, Joseph, Morera, Yolanda, Calvo, Belinda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm
http://hdl.handle.net/11449/67195
Resumo: A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
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spelling Molecular typing of IberoAmerican Cryptococcus neoformans isolatescontrolled studyCryptococcus neoformansDNA fingerprintingDNA polymorphismfungal genefungus isolationgenetic analysisgeographic distributionmolecular typingnonhumanpolymerase chain reactionrestriction fragment length polymorphismserotypeura5 genevirus geneAdultAgedAnimalsBacterial Typing TechniquesCentral AmericaCryptococcosisDNA FingerprintingEpidemiology, MolecularFemaleGoatsHumansOrotate PhosphoribosyltransferaseParrotsPhylogenyPolymorphism, GeneticPolymorphism, Restriction Fragment LengthSouth AmericaSpainA network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.University of Sydney, Sydney, NSWInstituto Nacional de Salud, BogotaUniversity of Western Sydney, Campbelltown, NSWMolecular Mycology Laboratory CIDM Westmead Hospital, Darcy Road, Westmead, NSW 2145Hosp. de Infecc. Francisco J. Muniz, Buenos AiresDepartamento Micologia Inst. Nac. Enferm. I. Dr. C.G.M., Buenos AiresLaboratorio de Micologia Medica Hospital Evandro Chagas FundaVao Oswaldo Cruz, Rio de JaneiroFaculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), AraraquaraAdolfo Lutz Inst. SeVao de Micologia, São PauloCentro de Biotechnologia (UFRGS), Porto AlegreProg. de Microbiologia y Micologia Universidad de Chile, SantiagoCorp. para Invest. Biologicas, MedellinInstituto Nacional de Salud, BogotáHospital San Juan de Dios, Guatemala CityUniversidad de San Carlos, Guatemala CityDept. de Microbiologia/Parasitologia Facultad de Medicina Univ. Nacional Autonoma de Mexico, México CityInst. Nac. de Diagn./Ref. Epidemiol., Mexico CityInst. de Med. Trop. A. Humboldt, LimaGrup Recerca en Micologia Exp./Clin. Inst. Munic. d'Investigacio Medica Universitat Autonoma de Barcelona, BarcelonaUniversidad del Zulia, MaracaiboFaculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP), AraraquaraUniversity of SydneyInstituto Nacional de SaludUniversity of Western SydneyWestmead HospitalHosp. de Infecc. Francisco J. MunizInst. Nac. Enferm. I. Dr. C.G.M.FundaVao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Instituto Adolfo Lutz (IAL)Universidade Federal do Rio Grande do Sul (UFRGS)Universidad de ChileCorp. para Invest. BiologicasHospital San Juan de DiosUniversidad de San CarlosUniv. Nacional Autonoma de MexicoInst. Nac. de Diagn./Ref. Epidemiol.Inst. de Med. Trop. A. HumboldtUniversitat Autonoma de BarcelonaUniversidad del ZuliaMeyer, WielandCastañeda, AlexandraJackson, StuartHuynh, MatthewCastañeda, ElizabethArechavala, AliciaDavel, GracielaRodero, LauraPerrotta, DiegoLazera, MarciaPereira-Igreja, RicardoWanke, BodoMendes-Giannini, Maria José Soares [UNESP]Melhem, Marcia S.C.Henning-Vainstein, MarleneDiaz, Maria CristinaRestrepo, AngelaHuérfano, SandraSamayoa, BlancaLogeman, HeidiMartirez, Rubén LópezOlivares, Laura Rocio CastañonContreras-Peres, CuadbertoTovar, José Francisco ValenzuelaBustamante, BeatrizTorres-Rodriquez, JosephMorera, YolandaCalvo, Belinda2014-05-27T11:20:36Z2014-05-27T11:20:36Z2003-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article189-195application/pdfhttp://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htmEmerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003.1080-6040http://hdl.handle.net/11449/671952-s2.0-03450741172-s2.0-0345074117.pdf0000-0002-8059-0826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEmerging Infectious Diseases7.4223,278info:eu-repo/semantics/openAccess2024-06-21T15:18:56Zoai:repositorio.unesp.br:11449/67195Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:24:09.949971Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular typing of IberoAmerican Cryptococcus neoformans isolates
title Molecular typing of IberoAmerican Cryptococcus neoformans isolates
spellingShingle Molecular typing of IberoAmerican Cryptococcus neoformans isolates
Meyer, Wieland
controlled study
Cryptococcus neoformans
DNA fingerprinting
DNA polymorphism
fungal gene
fungus isolation
genetic analysis
geographic distribution
molecular typing
nonhuman
polymerase chain reaction
restriction fragment length polymorphism
serotype
ura5 gene
virus gene
Adult
Aged
Animals
Bacterial Typing Techniques
Central America
Cryptococcosis
DNA Fingerprinting
Epidemiology, Molecular
Female
Goats
Humans
Orotate Phosphoribosyltransferase
Parrots
Phylogeny
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
South America
Spain
title_short Molecular typing of IberoAmerican Cryptococcus neoformans isolates
title_full Molecular typing of IberoAmerican Cryptococcus neoformans isolates
title_fullStr Molecular typing of IberoAmerican Cryptococcus neoformans isolates
title_full_unstemmed Molecular typing of IberoAmerican Cryptococcus neoformans isolates
title_sort Molecular typing of IberoAmerican Cryptococcus neoformans isolates
author Meyer, Wieland
author_facet Meyer, Wieland
Castañeda, Alexandra
Jackson, Stuart
Huynh, Matthew
Castañeda, Elizabeth
Arechavala, Alicia
Davel, Graciela
Rodero, Laura
Perrotta, Diego
Lazera, Marcia
Pereira-Igreja, Ricardo
Wanke, Bodo
Mendes-Giannini, Maria José Soares [UNESP]
Melhem, Marcia S.C.
Henning-Vainstein, Marlene
Diaz, Maria Cristina
Restrepo, Angela
Huérfano, Sandra
Samayoa, Blanca
Logeman, Heidi
Martirez, Rubén López
Olivares, Laura Rocio Castañon
Contreras-Peres, Cuadberto
Tovar, José Francisco Valenzuela
Bustamante, Beatriz
Torres-Rodriquez, Joseph
Morera, Yolanda
Calvo, Belinda
author_role author
author2 Castañeda, Alexandra
Jackson, Stuart
Huynh, Matthew
Castañeda, Elizabeth
Arechavala, Alicia
Davel, Graciela
Rodero, Laura
Perrotta, Diego
Lazera, Marcia
Pereira-Igreja, Ricardo
Wanke, Bodo
Mendes-Giannini, Maria José Soares [UNESP]
Melhem, Marcia S.C.
Henning-Vainstein, Marlene
Diaz, Maria Cristina
Restrepo, Angela
Huérfano, Sandra
Samayoa, Blanca
Logeman, Heidi
Martirez, Rubén López
Olivares, Laura Rocio Castañon
Contreras-Peres, Cuadberto
Tovar, José Francisco Valenzuela
Bustamante, Beatriz
Torres-Rodriquez, Joseph
Morera, Yolanda
Calvo, Belinda
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Sydney
Instituto Nacional de Salud
University of Western Sydney
Westmead Hospital
Hosp. de Infecc. Francisco J. Muniz
Inst. Nac. Enferm. I. Dr. C.G.M.
FundaVao Oswaldo Cruz
Universidade Estadual Paulista (Unesp)
Instituto Adolfo Lutz (IAL)
Universidade Federal do Rio Grande do Sul (UFRGS)
Universidad de Chile
Corp. para Invest. Biologicas
Hospital San Juan de Dios
Universidad de San Carlos
Univ. Nacional Autonoma de Mexico
Inst. Nac. de Diagn./Ref. Epidemiol.
Inst. de Med. Trop. A. Humboldt
Universitat Autonoma de Barcelona
Universidad del Zulia
dc.contributor.author.fl_str_mv Meyer, Wieland
Castañeda, Alexandra
Jackson, Stuart
Huynh, Matthew
Castañeda, Elizabeth
Arechavala, Alicia
Davel, Graciela
Rodero, Laura
Perrotta, Diego
Lazera, Marcia
Pereira-Igreja, Ricardo
Wanke, Bodo
Mendes-Giannini, Maria José Soares [UNESP]
Melhem, Marcia S.C.
Henning-Vainstein, Marlene
Diaz, Maria Cristina
Restrepo, Angela
Huérfano, Sandra
Samayoa, Blanca
Logeman, Heidi
Martirez, Rubén López
Olivares, Laura Rocio Castañon
Contreras-Peres, Cuadberto
Tovar, José Francisco Valenzuela
Bustamante, Beatriz
Torres-Rodriquez, Joseph
Morera, Yolanda
Calvo, Belinda
dc.subject.por.fl_str_mv controlled study
Cryptococcus neoformans
DNA fingerprinting
DNA polymorphism
fungal gene
fungus isolation
genetic analysis
geographic distribution
molecular typing
nonhuman
polymerase chain reaction
restriction fragment length polymorphism
serotype
ura5 gene
virus gene
Adult
Aged
Animals
Bacterial Typing Techniques
Central America
Cryptococcosis
DNA Fingerprinting
Epidemiology, Molecular
Female
Goats
Humans
Orotate Phosphoribosyltransferase
Parrots
Phylogeny
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
South America
Spain
topic controlled study
Cryptococcus neoformans
DNA fingerprinting
DNA polymorphism
fungal gene
fungus isolation
genetic analysis
geographic distribution
molecular typing
nonhuman
polymerase chain reaction
restriction fragment length polymorphism
serotype
ura5 gene
virus gene
Adult
Aged
Animals
Bacterial Typing Techniques
Central America
Cryptococcosis
DNA Fingerprinting
Epidemiology, Molecular
Female
Goats
Humans
Orotate Phosphoribosyltransferase
Parrots
Phylogeny
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
South America
Spain
description A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
publishDate 2003
dc.date.none.fl_str_mv 2003-02-01
2014-05-27T11:20:36Z
2014-05-27T11:20:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm
Emerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003.
1080-6040
http://hdl.handle.net/11449/67195
2-s2.0-0345074117
2-s2.0-0345074117.pdf
0000-0002-8059-0826
url http://wwwnc.cdc.gov/eid/article/9/2/02-0246_article.htm
http://hdl.handle.net/11449/67195
identifier_str_mv Emerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003.
1080-6040
2-s2.0-0345074117
2-s2.0-0345074117.pdf
0000-0002-8059-0826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Emerging Infectious Diseases
7.422
3,278
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 189-195
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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