Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/143485 |
Resumo: | Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days. Colonies compatible with mycobacteria were stained by Ziehl-Neelsen to detect acid-fast bacilli, while to confirm the Mycobacterium genus, conventional PCR was performed. Fourteen mycobacterial strains were isolated from 12 cows (8.4%). The hsp65 gene sequencing identified M. engbaekii (n=5), M. arupense (n=4), M. nonchromogenicum (n=3), and M. heraklionense (n=2) species belong to the Mycobacterium terrae complex. Despite the absence of M. tuberculosis complex species in the milk samples, identification of these mycobacteria highlights the risk of pathogen transmission from bovines to humans throughout milk or dairy products, since many of mycobacterial species described here have been reported in pulmonary and extrapulmonary diseases both in immunocompetent and immunocompromised people. |
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Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infectionsM. terrae complexBovineIntradermal tuberculin testsSequencing Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days. Colonies compatible with mycobacteria were stained by Ziehl-Neelsen to detect acid-fast bacilli, while to confirm the Mycobacterium genus, conventional PCR was performed. Fourteen mycobacterial strains were isolated from 12 cows (8.4%). The hsp65 gene sequencing identified M. engbaekii (n=5), M. arupense (n=4), M. nonchromogenicum (n=3), and M. heraklionense (n=2) species belong to the Mycobacterium terrae complex. Despite the absence of M. tuberculosis complex species in the milk samples, identification of these mycobacteria highlights the risk of pathogen transmission from bovines to humans throughout milk or dairy products, since many of mycobacterial species described here have been reported in pulmonary and extrapulmonary diseases both in immunocompetent and immunocompromised people.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/143485Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e6Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e6Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e61678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/143485/138164https://www.revistas.usp.br/rimtsp/article/view/143485/148509Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessBolaños, Carmen Alicia DazaFranco, Marília Masello JunqueiraSouza Filho, Antonio FranciscoIkuta, Cássia YumiBurbano-Rosero, Edith MarielaFerreira Neto, José SoaresHeinemann, Marcos BryanMotta, Rodrigo GarciaPaula, Carolina Lechinski deMorais, Amanda Bonalume Cordeiro deGuerra, Simony TrevizanAlves, Ana CarolinaListoni, Fernando José PaganiniRibeiro, Márcio Garcia2018-07-12T16:41:34Zoai:revistas.usp.br:article/143485Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2018-07-12T16:41:34Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false |
dc.title.none.fl_str_mv |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
title |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
spellingShingle |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections Bolaños, Carmen Alicia Daza M. terrae complex Bovine Intradermal tuberculin tests Sequencing |
title_short |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
title_full |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
title_fullStr |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
title_full_unstemmed |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
title_sort |
Nontuberculous mycobacteria in milk from positive cows in the intradermal comparative cervical tuberculin test: implications for human tuberculosis infections |
author |
Bolaños, Carmen Alicia Daza |
author_facet |
Bolaños, Carmen Alicia Daza Franco, Marília Masello Junqueira Souza Filho, Antonio Francisco Ikuta, Cássia Yumi Burbano-Rosero, Edith Mariela Ferreira Neto, José Soares Heinemann, Marcos Bryan Motta, Rodrigo Garcia Paula, Carolina Lechinski de Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Alves, Ana Carolina Listoni, Fernando José Paganini Ribeiro, Márcio Garcia |
author_role |
author |
author2 |
Franco, Marília Masello Junqueira Souza Filho, Antonio Francisco Ikuta, Cássia Yumi Burbano-Rosero, Edith Mariela Ferreira Neto, José Soares Heinemann, Marcos Bryan Motta, Rodrigo Garcia Paula, Carolina Lechinski de Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Alves, Ana Carolina Listoni, Fernando José Paganini Ribeiro, Márcio Garcia |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bolaños, Carmen Alicia Daza Franco, Marília Masello Junqueira Souza Filho, Antonio Francisco Ikuta, Cássia Yumi Burbano-Rosero, Edith Mariela Ferreira Neto, José Soares Heinemann, Marcos Bryan Motta, Rodrigo Garcia Paula, Carolina Lechinski de Morais, Amanda Bonalume Cordeiro de Guerra, Simony Trevizan Alves, Ana Carolina Listoni, Fernando José Paganini Ribeiro, Márcio Garcia |
dc.subject.por.fl_str_mv |
M. terrae complex Bovine Intradermal tuberculin tests Sequencing |
topic |
M. terrae complex Bovine Intradermal tuberculin tests Sequencing |
description |
Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days. Colonies compatible with mycobacteria were stained by Ziehl-Neelsen to detect acid-fast bacilli, while to confirm the Mycobacterium genus, conventional PCR was performed. Fourteen mycobacterial strains were isolated from 12 cows (8.4%). The hsp65 gene sequencing identified M. engbaekii (n=5), M. arupense (n=4), M. nonchromogenicum (n=3), and M. heraklionense (n=2) species belong to the Mycobacterium terrae complex. Despite the absence of M. tuberculosis complex species in the milk samples, identification of these mycobacteria highlights the risk of pathogen transmission from bovines to humans throughout milk or dairy products, since many of mycobacterial species described here have been reported in pulmonary and extrapulmonary diseases both in immunocompetent and immunocompromised people. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/143485 |
url |
https://www.revistas.usp.br/rimtsp/article/view/143485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/143485/138164 https://www.revistas.usp.br/rimtsp/article/view/143485/148509 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e6 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 60 (2018); e6 Revista do Instituto de Medicina Tropical de São Paulo; v. 60 (2018); e6 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1752129959370948608 |