Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Yoshida, N.
Data de Publicação: 1986
Outros Autores: Teixeira, M. M. G., Sbravate, C. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/87468
Resumo: Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.
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spelling Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi Caracterização antigênica de tripomastigotas metacíclicos de Trypanosoma cruzi provenientes do vetor e de cultura Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction. Tripomastigotas metacíclicos da cepa CL de Trypanosoma cruzi, originários de triatomineos e de cultura axênica, foram comparativamente analisados quanto a sua composição antigênica e características ímunogênicas. Os vários parâmetros examinados mostraram que as duas formas metacíclicas sáo semelhantes. Assim, soros de camundongos imunizados com qualquer uma das formas metacíclicas precipitaram uma proteína de superfície de 82Kd de tripomastigotas metacíclicos de cultura marcados com 131I. Soros de camundongos protegidos con trn infecção aguda por T. cruzi, por imunização com formas metacíclicas de cultura inativadas, de uma cepa diferente (G), detectaram por imunoblotting uma proteína de 77Kd em ambas as formas metacíclicas da cepa CL. Um anticorpo monoclonal, produzido contra as for mas metaciclicas da cepa G, e específico para tripomastigotas metacíclicos, reagiu com formas metacíclicas da cepa CL tanto do vetor quanto de cultura. As duas formas metacíclicas mostraram suscetibilidade semelhante à lise por vários soros anti-T. cruzi, em uma reação mediada por complemento. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1986-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/87468Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 No. 2 (1986); 80-86Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 Núm. 2 (1986); 80-86Revista do Instituto de Medicina Tropical de São Paulo; v. 28 n. 2 (1986); 80-861678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/87468/90426Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessYoshida, N.Teixeira, M. M. G.Sbravate, C. A.2014-11-07T18:33:33Zoai:revistas.usp.br:article/87468Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:26.209900Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
Caracterização antigênica de tripomastigotas metacíclicos de Trypanosoma cruzi provenientes do vetor e de cultura
title Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
spellingShingle Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
Yoshida, N.
title_short Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
title_full Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
title_fullStr Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
title_full_unstemmed Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
title_sort Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi
author Yoshida, N.
author_facet Yoshida, N.
Teixeira, M. M. G.
Sbravate, C. A.
author_role author
author2 Teixeira, M. M. G.
Sbravate, C. A.
author2_role author
author
dc.contributor.author.fl_str_mv Yoshida, N.
Teixeira, M. M. G.
Sbravate, C. A.
description Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.
publishDate 1986
dc.date.none.fl_str_mv 1986-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/87468
url https://www.revistas.usp.br/rimtsp/article/view/87468
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/87468/90426
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 No. 2 (1986); 80-86
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 28 Núm. 2 (1986); 80-86
Revista do Instituto de Medicina Tropical de São Paulo; v. 28 n. 2 (1986); 80-86
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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