Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies
Autor(a) principal: | |
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Data de Publicação: | 1987 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28508 |
Resumo: | Nine samples of Plasmodium falciparum were collected from migrant workers who were infected in the State of Rondônia, Brazil. These samples were maintained in culture for characterization by enzyme typing using acetate cellulose electrophoresis, drug susceptibility to chlo-roquine, amodiaquine, mefloquine and quinine and antigenic diversity analysis using strain specific monoclonal antibodies. The results obtained have confirmed major variation among all samples studied: it was found consistent resistance to chloroquine moderated resistance to amodiaquine and quinine and susceptibility to low levels of mefloquine; only two isolates have shown susceptibility to all drugs in the in vitra assays. Enzyme typing showed presence of both GPI 1 and 2 and ADA 1 and 2 parasites, while for PEP and LDH all samples were type 1. Se-rotyping with PSA monoclonal antibodies showed presence of three different serotypes (II, III and IV). These results confirm: a) variation among samples for markers analysed; b) in this area and for the small number of samples studied, no significant differences or new types of parasites were seen. |
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Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies Caracterização de cepas de plasmodium falciparum do Estado de Rondônia, Brasil, utilizando microtestes de sensibilidade aos antimaláricos, tipificação enzimática e anticorpos monoclonais MaláriaCaracterização de cepasP. falciparum Nine samples of Plasmodium falciparum were collected from migrant workers who were infected in the State of Rondônia, Brazil. These samples were maintained in culture for characterization by enzyme typing using acetate cellulose electrophoresis, drug susceptibility to chlo-roquine, amodiaquine, mefloquine and quinine and antigenic diversity analysis using strain specific monoclonal antibodies. The results obtained have confirmed major variation among all samples studied: it was found consistent resistance to chloroquine moderated resistance to amodiaquine and quinine and susceptibility to low levels of mefloquine; only two isolates have shown susceptibility to all drugs in the in vitra assays. Enzyme typing showed presence of both GPI 1 and 2 and ADA 1 and 2 parasites, while for PEP and LDH all samples were type 1. Se-rotyping with PSA monoclonal antibodies showed presence of three different serotypes (II, III and IV). These results confirm: a) variation among samples for markers analysed; b) in this area and for the small number of samples studied, no significant differences or new types of parasites were seen. Nove amostras de Plasmodium falciparum foram coletadas de migrantes infectados no Estado de Rondônia. Estas amostras foram mantidas em cultivo para caracterização por tipificação enzimática em acetato de celulose, sensibilidade á cloroquina, amodiaquina, mefloquina e quinino e análise da diversidade antigê-nica através de anticorpos monoclonais específicos. Os resultados obtidos mostraram variação entre todas as amostras estudadas: encontrou-se resistência crescente à cloroquina, resistência intermediária à amodiaquina e quinino e sensibilidade a baixos níveis de mefloquina; apenas dois isolados mostraram sensibilidade a todas as drogas. A tipificação enzimática mostrou presença de parasitas GPI 1 e 2 e ADA 1 e 2, enquanto que para PEP e LDH todas as amostras foram do tipo 1. Sorotipagem com anticorpos monoclonais PSA mostrou presença de três sorotipos diferentes (II, III e IV). Estes resultados mostraram: a) variação entre as amostras para os marcadores analisados; b) nesta região, para o pequeno número de amostras analisadas, não foram observadas diferenças significativas ou novos tipos de parasitas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1987-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28508Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 3 (1987); 142-147 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 3 (1987); 142-147 Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 3 (1987); 142-147 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28508/30361Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDi Santi, S. M.Boulos, M.Vasconcelos, M. A.Oliveira, S.Couto, A.Rosario, V. E.2012-07-02T00:57:23Zoai:revistas.usp.br:article/28508Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:14.277336Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies Caracterização de cepas de plasmodium falciparum do Estado de Rondônia, Brasil, utilizando microtestes de sensibilidade aos antimaláricos, tipificação enzimática e anticorpos monoclonais |
title |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
spellingShingle |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies Di Santi, S. M. Malária Caracterização de cepas P. falciparum |
title_short |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
title_full |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
title_fullStr |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
title_full_unstemmed |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
title_sort |
Characterization of Plasmodium falciparum isolates from Rondônia State, Brazil, by drug susceptibility, enzyme typing and monoclonal antibodies |
author |
Di Santi, S. M. |
author_facet |
Di Santi, S. M. Boulos, M. Vasconcelos, M. A. Oliveira, S. Couto, A. Rosario, V. E. |
author_role |
author |
author2 |
Boulos, M. Vasconcelos, M. A. Oliveira, S. Couto, A. Rosario, V. E. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Di Santi, S. M. Boulos, M. Vasconcelos, M. A. Oliveira, S. Couto, A. Rosario, V. E. |
dc.subject.por.fl_str_mv |
Malária Caracterização de cepas P. falciparum |
topic |
Malária Caracterização de cepas P. falciparum |
description |
Nine samples of Plasmodium falciparum were collected from migrant workers who were infected in the State of Rondônia, Brazil. These samples were maintained in culture for characterization by enzyme typing using acetate cellulose electrophoresis, drug susceptibility to chlo-roquine, amodiaquine, mefloquine and quinine and antigenic diversity analysis using strain specific monoclonal antibodies. The results obtained have confirmed major variation among all samples studied: it was found consistent resistance to chloroquine moderated resistance to amodiaquine and quinine and susceptibility to low levels of mefloquine; only two isolates have shown susceptibility to all drugs in the in vitra assays. Enzyme typing showed presence of both GPI 1 and 2 and ADA 1 and 2 parasites, while for PEP and LDH all samples were type 1. Se-rotyping with PSA monoclonal antibodies showed presence of three different serotypes (II, III and IV). These results confirm: a) variation among samples for markers analysed; b) in this area and for the small number of samples studied, no significant differences or new types of parasites were seen. |
publishDate |
1987 |
dc.date.none.fl_str_mv |
1987-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28508 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28508 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28508/30361 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 3 (1987); 142-147 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 3 (1987); 142-147 Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 3 (1987); 142-147 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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