PCR - based diagnosis to evaluate the performance of malaria reference centers
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30821 |
Resumo: | Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively. |
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PCR - based diagnosis to evaluate the performance of malaria reference centers Diagnóstico baseado em PCR para avaliar o desempenho de centros de referência em malária MalariaDiagnosisPlasmodiumPCRssrRNAThick blood smear Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively. Embora a gota espessa corada por Giemsa (GTS) permaneça o padrão ouro para o diagnóstico de malária, métodos moleculares são mais sensíveis e específicos para detectar parasitas e podem ser utilizados em centros de referência para avaliar o desempenho da microscopia. A descrição das seqüências dos genes ssrRNA de Plasmodium falciparum, P. vivax, P. malariae e P. ovale permitiu o desenvolvimento de uma reação em cadeia da polimerase (PCR) que tem sido utilizada para diferenciar as quatro espécies. O objetivo deste estudo foi determinar as espécies de Plasmodium através de PCR em 190 lâminas positivas de pacientes para verificar a qualidade do diagnóstico realizado no Laboratório de Malária da SUCEN. Considerando somente os 131 resultados positivos em ambas as técnicas, GTS detectou 4,6% de infecções mistas e 3,1% de P. malariae enquanto o PCR identificou 19,1% e 13,8%, respectivamente. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30821Revista do Instituto de Medicina Tropical de São Paulo; Vol. 46 No. 4 (2004); 183-187 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 46 Núm. 4 (2004); 183-187 Revista do Instituto de Medicina Tropical de São Paulo; v. 46 n. 4 (2004); 183-187 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30821/32705Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDi Santi, Silvia MariaKirchgatter, KarinBrunialti, Karen Cristina Sant'AnnaOliveira, Alessandra MotaFerreira, Sergio Roberto SantosBoulos, Marcos2012-07-07T18:28:51Zoai:revistas.usp.br:article/30821Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:34.090692Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
PCR - based diagnosis to evaluate the performance of malaria reference centers Diagnóstico baseado em PCR para avaliar o desempenho de centros de referência em malária |
title |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
spellingShingle |
PCR - based diagnosis to evaluate the performance of malaria reference centers Di Santi, Silvia Maria Malaria Diagnosis Plasmodium PCR ssrRNA Thick blood smear |
title_short |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
title_full |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
title_fullStr |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
title_full_unstemmed |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
title_sort |
PCR - based diagnosis to evaluate the performance of malaria reference centers |
author |
Di Santi, Silvia Maria |
author_facet |
Di Santi, Silvia Maria Kirchgatter, Karin Brunialti, Karen Cristina Sant'Anna Oliveira, Alessandra Mota Ferreira, Sergio Roberto Santos Boulos, Marcos |
author_role |
author |
author2 |
Kirchgatter, Karin Brunialti, Karen Cristina Sant'Anna Oliveira, Alessandra Mota Ferreira, Sergio Roberto Santos Boulos, Marcos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Di Santi, Silvia Maria Kirchgatter, Karin Brunialti, Karen Cristina Sant'Anna Oliveira, Alessandra Mota Ferreira, Sergio Roberto Santos Boulos, Marcos |
dc.subject.por.fl_str_mv |
Malaria Diagnosis Plasmodium PCR ssrRNA Thick blood smear |
topic |
Malaria Diagnosis Plasmodium PCR ssrRNA Thick blood smear |
description |
Although the Giemsa-stained thick blood smear (GTS) remains the gold standard for the diagnosis of malaria, molecular methods are more sensitive and specific to detect parasites and can be used at reference centers to evaluate the performance of microscopy. The description of the Plasmodium falciparum, P. vivax, P. malariae and P. ovale ssrRNA gene sequences allowed the development of a polymerase chain reaction (PCR) that had been used to differentiate the four species. The objective of this study was to determine Plasmodium species through PCR in 190 positive smears from patients in order to verify the quality of diagnosis at SUCEN's Malaria Laboratory. Considering only the 131 positive results in both techniques, GTS detected 4.6% of mixed and 3.1% of P. malariae infections whereas PCR identified 19.1% and 13.8%, respectively. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30821 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30821 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30821/32705 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 46 No. 4 (2004); 183-187 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 46 Núm. 4 (2004); 183-187 Revista do Instituto de Medicina Tropical de São Paulo; v. 46 n. 4 (2004); 183-187 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951644575039488 |