In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms
Autor(a) principal: | |
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Data de Publicação: | 1991 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28790 |
Resumo: | The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media. |
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In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms Ação "in vitro" de alguns desinfetantes sobre formas leveduriformes de Paracoccidioides brasiliensis Paracoccidioides brasiliensisDisinfectantsFluorescein diacetate-ethidium bromideYeast to mycelial germinationRadiometric methodSodium hypochlorite The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media. Descreveu-se a ação fungicida do hipoclorito de sódio (0,3; 1; 2,5; 5 e 10%); do formaldeído (em solução aquosa a 2,5 e 10%); e álcool etílico a 70,0% sobre formas leveduriformes de 2 cepas de Paraccoccidioides brasiliensis: Pb 18 e cepa Goiana, recentemente isolada. A incubação do fungo e desinfetantes foi realizada à temperatura ambiente por períodos de 1, 2, 24, 48 e 72 horas. A viabilidade foi avaliada pelo tratamento com diacetato de fluoresceína-brometo de etídeo; pela cultura em meios sólidos e líquidos a 36ºC e 26ºC; transformação de levedura em micélio à temperatura ambiente; e estudo radiométrico da atividade metabólica. Todas as concentrações de todos os desinfetantes estudados foram capazes de inativar ambas as cepas, exceto na incubação com formaldeído a 2% por 1 hora, em que o tratamento por diacetato de fluoresceína-brometo de etídeo revelou 40% e 27% de células viáveis, respectivamente, para a cepa Pb 18 e Goiana. A transformação de levedura em micélio foi considerada um método rápido, com resultados semelhantes ao cultivo em meios sólidos e líquidos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28790Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 1 (1991); 37-43 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 1 (1991); 37-43 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 1 (1991); 37-43 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28790/30643Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessShikanai-Yasuda, M. A.Taguchi, R. T.Sato, M. K.Melo, N. T.Assis, C. M.Nigro, R. C.Camargo, E. E.Lacaz, C. S.Amato Neto, V.Sesso, A.2012-07-02T01:29:49Zoai:revistas.usp.br:article/28790Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:33.132674Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms Ação "in vitro" de alguns desinfetantes sobre formas leveduriformes de Paracoccidioides brasiliensis |
title |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
spellingShingle |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms Shikanai-Yasuda, M. A. Paracoccidioides brasiliensis Disinfectants Fluorescein diacetate-ethidium bromide Yeast to mycelial germination Radiometric method Sodium hypochlorite |
title_short |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
title_full |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
title_fullStr |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
title_full_unstemmed |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
title_sort |
In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms |
author |
Shikanai-Yasuda, M. A. |
author_facet |
Shikanai-Yasuda, M. A. Taguchi, R. T. Sato, M. K. Melo, N. T. Assis, C. M. Nigro, R. C. Camargo, E. E. Lacaz, C. S. Amato Neto, V. Sesso, A. |
author_role |
author |
author2 |
Taguchi, R. T. Sato, M. K. Melo, N. T. Assis, C. M. Nigro, R. C. Camargo, E. E. Lacaz, C. S. Amato Neto, V. Sesso, A. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Shikanai-Yasuda, M. A. Taguchi, R. T. Sato, M. K. Melo, N. T. Assis, C. M. Nigro, R. C. Camargo, E. E. Lacaz, C. S. Amato Neto, V. Sesso, A. |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis Disinfectants Fluorescein diacetate-ethidium bromide Yeast to mycelial germination Radiometric method Sodium hypochlorite |
topic |
Paracoccidioides brasiliensis Disinfectants Fluorescein diacetate-ethidium bromide Yeast to mycelial germination Radiometric method Sodium hypochlorite |
description |
The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinfectants were found to be effective in inactivating Pb 18 and Goiana strains, except for the 1-hour contact with 2% formaldehyde, in which fluorescein diacetate-ethidium bromide treatment was found to reveal 40 and 27% of viable cells, respectively. The yeast to mycelial germination method was considered to reveal faster and similar results as compared to culture in solid and liquid media. |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28790 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28790 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28790/30643 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 1 (1991); 37-43 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 1 (1991); 37-43 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 1 (1991); 37-43 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951638837231616 |