THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/53549 |
Resumo: | This study investigated the sero-conversion period in which dogs from endemic areas test positive for visceral leishmaniasis (VL) as well as the early post-infection period in which renal alterations are observed. Dogs that were initially negative for Canine Visceral Leishmaniasis (CVL) were clinically evaluated every three months by serological, parasitological and biochemical tests until sero-conversion was confirmed, and six months later a subsequent evaluation was performed. Samples of kidney tissues were processed and stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS) and Masson’s trichrome stain and lesions were classified based on the WHO criteria. Of the 40 dogs that initially tested negative for VL, 25 (62.5%) exhibited positive serological tests during the study period. Of these 25 dogs, 15 (60%) tested positive within three months, five (20%) tested positive within six months and five (20%) tested positive within nine months. The dogs exhibited antibody titers between 1:40 and 1:80 and 72% of the dogs exhibited clinical symptoms. The Leishmania antigen was present in the kidneys of recently infected dogs. We found higher levels of total protein and globulin as well as lower levels of albumin in the infected dogs when compared to the control dogs. Additionally, infected dogs presented levels of urea and creatinine that were higher than those of the uninfected dogs. Glomerulonephritis was detected in some of the dogs examined in this study. These data suggest that in Teresina, the sero-conversion for VL occurs quickly and showed that the infected dogs presented abnormal serum proteins, as well as structural and functional alterations in the kidneys during the early post-infection period. |
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THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasiSoro-conversão e avaliação das alterações renais em cães infectados por Leishmania (Infantum) chagasiVisceral LeishmaniasisSeroconversionDogsKidneyThis study investigated the sero-conversion period in which dogs from endemic areas test positive for visceral leishmaniasis (VL) as well as the early post-infection period in which renal alterations are observed. Dogs that were initially negative for Canine Visceral Leishmaniasis (CVL) were clinically evaluated every three months by serological, parasitological and biochemical tests until sero-conversion was confirmed, and six months later a subsequent evaluation was performed. Samples of kidney tissues were processed and stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS) and Masson’s trichrome stain and lesions were classified based on the WHO criteria. Of the 40 dogs that initially tested negative for VL, 25 (62.5%) exhibited positive serological tests during the study period. Of these 25 dogs, 15 (60%) tested positive within three months, five (20%) tested positive within six months and five (20%) tested positive within nine months. The dogs exhibited antibody titers between 1:40 and 1:80 and 72% of the dogs exhibited clinical symptoms. The Leishmania antigen was present in the kidneys of recently infected dogs. We found higher levels of total protein and globulin as well as lower levels of albumin in the infected dogs when compared to the control dogs. Additionally, infected dogs presented levels of urea and creatinine that were higher than those of the uninfected dogs. Glomerulonephritis was detected in some of the dogs examined in this study. These data suggest that in Teresina, the sero-conversion for VL occurs quickly and showed that the infected dogs presented abnormal serum proteins, as well as structural and functional alterations in the kidneys during the early post-infection period.Este estudo investigou o período em que o cão torna-se positivo para leishmaniose visceral (VL) em área endêmica e as alterações renais no período recente pós-infecção. Cães negativos para VL foram avaliados clinicamente a cada três meses por testes sorológicos, parasitológicos e bioquímicos até a soro-conversão e seis meses após. Foram colhidos tecido renal de seis cães, submetidos a processamento de rotina e corados com HE, PAS e Masson e as lesões foram classificadas com base nos critérios da OMS. Dos 40 cães nagativos para VL, 25 (62,5%) apresentaram sorologia positiva durante o estudo. Desses, 15 (60%) tornaram-se positiva nos primeiros três meses, cinco (20%) tornaram-se positivas dentro de seis meses e cinco (20%) tornaram-se positivas dentro de nove meses. Os cães apresentavam títulos de anticorpos entre 1:40 e 1:80, e 72% mostraram sinais clínicos. Antígeno de Leishmania estava presente no rim. Foram encontrados níveis mais elevados de proteína total e globulina, e menores níveis de albumina em cães infectados quando comparados aos controles. Além disso, os cães infectados apresentaram níveis de uréia e creatinina maior do que os cães controles. Glomerulonefrite foi observada em cinco cães. Os resultados sugerem que em Teresina a soro-conversão para VL ocorre rapidamente e os cães apresentam proteínas séricas anormais e alterações na estrutura e função dos rins em um período recente pós-infecção.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/53549Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 2 (2013); 105-112Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 2 (2013); 105-112Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 2 (2013); 105-1121678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/53549/77129Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessAlves, Georgia Brenda BarrosSilva, Lucilene dos SantosBatista, Joilson FerreiraCampos, Ângela PiauilinoPrianti, Maria das GraçasCosta, Francisco Assis Lima2014-01-30T12:29:10Zoai:revistas.usp.br:article/53549Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:12.382309Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi Soro-conversão e avaliação das alterações renais em cães infectados por Leishmania (Infantum) chagasi |
title |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
spellingShingle |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi Alves, Georgia Brenda Barros Visceral Leishmaniasis Seroconversion Dogs Kidney |
title_short |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
title_full |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
title_fullStr |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
title_full_unstemmed |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
title_sort |
THE SERO-CONVERSION AND EVALUATION OF RENAL ALTERATIONS IN DOGS INFECTED BY Leishmania (Infantum) chagasi |
author |
Alves, Georgia Brenda Barros |
author_facet |
Alves, Georgia Brenda Barros Silva, Lucilene dos Santos Batista, Joilson Ferreira Campos, Ângela Piauilino Prianti, Maria das Graças Costa, Francisco Assis Lima |
author_role |
author |
author2 |
Silva, Lucilene dos Santos Batista, Joilson Ferreira Campos, Ângela Piauilino Prianti, Maria das Graças Costa, Francisco Assis Lima |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Georgia Brenda Barros Silva, Lucilene dos Santos Batista, Joilson Ferreira Campos, Ângela Piauilino Prianti, Maria das Graças Costa, Francisco Assis Lima |
dc.subject.por.fl_str_mv |
Visceral Leishmaniasis Seroconversion Dogs Kidney |
topic |
Visceral Leishmaniasis Seroconversion Dogs Kidney |
description |
This study investigated the sero-conversion period in which dogs from endemic areas test positive for visceral leishmaniasis (VL) as well as the early post-infection period in which renal alterations are observed. Dogs that were initially negative for Canine Visceral Leishmaniasis (CVL) were clinically evaluated every three months by serological, parasitological and biochemical tests until sero-conversion was confirmed, and six months later a subsequent evaluation was performed. Samples of kidney tissues were processed and stained with Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS) and Masson’s trichrome stain and lesions were classified based on the WHO criteria. Of the 40 dogs that initially tested negative for VL, 25 (62.5%) exhibited positive serological tests during the study period. Of these 25 dogs, 15 (60%) tested positive within three months, five (20%) tested positive within six months and five (20%) tested positive within nine months. The dogs exhibited antibody titers between 1:40 and 1:80 and 72% of the dogs exhibited clinical symptoms. The Leishmania antigen was present in the kidneys of recently infected dogs. We found higher levels of total protein and globulin as well as lower levels of albumin in the infected dogs when compared to the control dogs. Additionally, infected dogs presented levels of urea and creatinine that were higher than those of the uninfected dogs. Glomerulonephritis was detected in some of the dogs examined in this study. These data suggest that in Teresina, the sero-conversion for VL occurs quickly and showed that the infected dogs presented abnormal serum proteins, as well as structural and functional alterations in the kidneys during the early post-infection period. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/53549 |
url |
https://www.revistas.usp.br/rimtsp/article/view/53549 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/53549/77129 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 2 (2013); 105-112 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 2 (2013); 105-112 Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 2 (2013); 105-112 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951648585842688 |