Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf http://hdl.handle.net/11449/73720 |
Resumo: | Although canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis. |
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Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasiHistopathologyImmunohistochemistryMuscleVisceral leishmaniasisCanis familiarisLeishmania infantumAlthough canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis.Agricultural Science Center Federal University of São Francisco Valley, Petrolina, PEUniversity of São Paulo Medical School, São Paulo, SPSão Paulo State University School of Veterinary Medicine, Jaboticabal, São PauloSão Paulo State University School of Veterinary Medicine, Araçatuba, São PauloSão Paulo State University School of Veterinary Medicine, Jaboticabal, São PauloSão Paulo State University School of Veterinary Medicine, Araçatuba, São PauloFederal University of São Francisco ValleyUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Gomes, Ana Amélia Domingues [UNESP]Laurenti, Márcia DalastraFerraro, Gisela Cristiane [UNESP]de Camargo, Mauro Henrique Bueno [UNESP]Costa, Denis Carvalho [UNESP]Machado, Gisele Fabrino [UNESP]Perri, Silvia Helena Venturoli [UNESP]Marcondes, Mary [UNESP]2014-05-27T11:27:08Z2014-05-27T11:27:08Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-115http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdfBrazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012.1983-0246http://hdl.handle.net/11449/737202-s2.0-84870935347031040555812563408108375982026341817946671090010Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathology0,162info:eu-repo/semantics/openAccess2024-09-04T19:16:19Zoai:repositorio.unesp.br:11449/73720Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
title |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
spellingShingle |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi Gomes, Ana Amélia Domingues [UNESP] Histopathology Immunohistochemistry Muscle Visceral leishmaniasis Canis familiaris Leishmania infantum |
title_short |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
title_full |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
title_fullStr |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
title_full_unstemmed |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
title_sort |
Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi |
author |
Gomes, Ana Amélia Domingues [UNESP] |
author_facet |
Gomes, Ana Amélia Domingues [UNESP] Laurenti, Márcia Dalastra Ferraro, Gisela Cristiane [UNESP] de Camargo, Mauro Henrique Bueno [UNESP] Costa, Denis Carvalho [UNESP] Machado, Gisele Fabrino [UNESP] Perri, Silvia Helena Venturoli [UNESP] Marcondes, Mary [UNESP] |
author_role |
author |
author2 |
Laurenti, Márcia Dalastra Ferraro, Gisela Cristiane [UNESP] de Camargo, Mauro Henrique Bueno [UNESP] Costa, Denis Carvalho [UNESP] Machado, Gisele Fabrino [UNESP] Perri, Silvia Helena Venturoli [UNESP] Marcondes, Mary [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of São Francisco Valley Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes, Ana Amélia Domingues [UNESP] Laurenti, Márcia Dalastra Ferraro, Gisela Cristiane [UNESP] de Camargo, Mauro Henrique Bueno [UNESP] Costa, Denis Carvalho [UNESP] Machado, Gisele Fabrino [UNESP] Perri, Silvia Helena Venturoli [UNESP] Marcondes, Mary [UNESP] |
dc.subject.por.fl_str_mv |
Histopathology Immunohistochemistry Muscle Visceral leishmaniasis Canis familiaris Leishmania infantum |
topic |
Histopathology Immunohistochemistry Muscle Visceral leishmaniasis Canis familiaris Leishmania infantum |
description |
Although canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2014-05-27T11:27:08Z 2014-05-27T11:27:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf Brazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012. 1983-0246 http://hdl.handle.net/11449/73720 2-s2.0-84870935347 0310405558125634 0810837598202634 1817946671090010 |
url |
http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf http://hdl.handle.net/11449/73720 |
identifier_str_mv |
Brazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012. 1983-0246 2-s2.0-84870935347 0310405558125634 0810837598202634 1817946671090010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Pathology 0,162 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
108-115 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021424753016832 |