Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi

Detalhes bibliográficos
Autor(a) principal: Gomes, Ana Amélia Domingues [UNESP]
Data de Publicação: 2012
Outros Autores: Laurenti, Márcia Dalastra, Ferraro, Gisela Cristiane [UNESP], de Camargo, Mauro Henrique Bueno [UNESP], Costa, Denis Carvalho [UNESP], Machado, Gisele Fabrino [UNESP], Perri, Silvia Helena Venturoli [UNESP], Marcondes, Mary [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf
http://hdl.handle.net/11449/73720
Resumo: Although canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis.
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spelling Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasiHistopathologyImmunohistochemistryMuscleVisceral leishmaniasisCanis familiarisLeishmania infantumAlthough canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis.Agricultural Science Center Federal University of São Francisco Valley, Petrolina, PEUniversity of São Paulo Medical School, São Paulo, SPSão Paulo State University School of Veterinary Medicine, Jaboticabal, São PauloSão Paulo State University School of Veterinary Medicine, Araçatuba, São PauloSão Paulo State University School of Veterinary Medicine, Jaboticabal, São PauloSão Paulo State University School of Veterinary Medicine, Araçatuba, São PauloFederal University of São Francisco ValleyUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Gomes, Ana Amélia Domingues [UNESP]Laurenti, Márcia DalastraFerraro, Gisela Cristiane [UNESP]de Camargo, Mauro Henrique Bueno [UNESP]Costa, Denis Carvalho [UNESP]Machado, Gisele Fabrino [UNESP]Perri, Silvia Helena Venturoli [UNESP]Marcondes, Mary [UNESP]2014-05-27T11:27:08Z2014-05-27T11:27:08Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-115http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdfBrazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012.1983-0246http://hdl.handle.net/11449/737202-s2.0-84870935347031040555812563408108375982026341817946671090010Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathology0,162info:eu-repo/semantics/openAccess2024-09-04T19:16:19Zoai:repositorio.unesp.br:11449/73720Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
title Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
spellingShingle Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
Gomes, Ana Amélia Domingues [UNESP]
Histopathology
Immunohistochemistry
Muscle
Visceral leishmaniasis
Canis familiaris
Leishmania infantum
title_short Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
title_full Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
title_fullStr Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
title_full_unstemmed Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
title_sort Subclinical muscle injuries in dogs infected with Leishmania (Leishmania) infantum chagasi
author Gomes, Ana Amélia Domingues [UNESP]
author_facet Gomes, Ana Amélia Domingues [UNESP]
Laurenti, Márcia Dalastra
Ferraro, Gisela Cristiane [UNESP]
de Camargo, Mauro Henrique Bueno [UNESP]
Costa, Denis Carvalho [UNESP]
Machado, Gisele Fabrino [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Marcondes, Mary [UNESP]
author_role author
author2 Laurenti, Márcia Dalastra
Ferraro, Gisela Cristiane [UNESP]
de Camargo, Mauro Henrique Bueno [UNESP]
Costa, Denis Carvalho [UNESP]
Machado, Gisele Fabrino [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Marcondes, Mary [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of São Francisco Valley
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gomes, Ana Amélia Domingues [UNESP]
Laurenti, Márcia Dalastra
Ferraro, Gisela Cristiane [UNESP]
de Camargo, Mauro Henrique Bueno [UNESP]
Costa, Denis Carvalho [UNESP]
Machado, Gisele Fabrino [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Marcondes, Mary [UNESP]
dc.subject.por.fl_str_mv Histopathology
Immunohistochemistry
Muscle
Visceral leishmaniasis
Canis familiaris
Leishmania infantum
topic Histopathology
Immunohistochemistry
Muscle
Visceral leishmaniasis
Canis familiaris
Leishmania infantum
description Although canine visceral leishmaniasis (CVL) has been extensively studied, muscular damage due to Leishmania (Leishmania) infantum chagasi infection remains to be fully established. The aim of this study was to describe the electromyographic and histological changes, as well as search for the presence of amastigote forms of Leishmania spp, CD3+ T-lymphocytes, macrophages and IgG in skeletal muscles of dogs with visceral leishmaniasis (VL). Four muscles (triceps brachial, extensor carpi radialis, biceps femoris and gastrocnemius) from a total of 17 naturally infected and six healthy dogs were used in this study. Electromyographic alterations such as fibrillation potentials, positive sharp waves and complex repetitive discharges were observed in, at least, three muscles from all infected dogs. Myocyte necrosis and degeneration were the most frequent muscular injury seen, followed by inflammatory reaction, fibrosis and variation in muscle fibers size. Immunohistochemistry in muscle samples revealed amastigote forms in 4/17 (23. 53%), IgG in 12/17 (70. 58%), CD3+ T-lymphocytes in 16/17 (94. 12%) and macrophages in 17/17 (100%) dogs. Statistically positive correlation was observed between: inflammatory infiltrate (p=0. 0305) and CD3+ immunoreaction (p=0. 0307) in relation to the number of amastigote forms; inflammatory infiltrate (p=0. 0101) and macrophage immunoreaction (p=0. 0127) in relation to the amount of CD3+; and inflammatory infiltrate (p=0. 0044) and degeneration/necrosis (p<0. 0001) in relation to the presence of macrophages. Our results suggest that different mechanisms contribute to the development of myocytotoxicity, including celular and humoral immune responses and direct muscular injury by the parasite. Nevertheless, the catabolic nature of the disease can probably interact with other factors, but cannot be incriminated as the only responsible for myositis.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
2014-05-27T11:27:08Z
2014-05-27T11:27:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf
Brazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012.
1983-0246
http://hdl.handle.net/11449/73720
2-s2.0-84870935347
0310405558125634
0810837598202634
1817946671090010
url http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-24-20881_2012_11_30_40_58.pdf
http://hdl.handle.net/11449/73720
identifier_str_mv Brazilian Journal of Veterinary Pathology, v. 5, n. 3, p. 108-115, 2012.
1983-0246
2-s2.0-84870935347
0310405558125634
0810837598202634
1817946671090010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Veterinary Pathology
0,162
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 108-115
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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