A critical evaluation of the expression of parasitemia in experimental Chagas' disease
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29075 |
Resumo: | This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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A critical evaluation of the expression of parasitemia in experimental Chagas' disease Estudo crítico sobre a expressão da parasitemia na doença de Chagas experimental Trypanosoma cruziParasitemiaCritical evaluation This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used. O objetivo deste trabalho foi o de estudar a melhor maneira de expressar a parasitemia de animais experimentalmente infectados com T. cruzi, visto que os animais podem apresentar grande variabilidade nos dados de parasitemia e que não é expressa nos resultados da maioria dos autores. Nós usamos um grupo de 50 ratos que foram infectados com 1x10(6) tripomastigotas da cepa Y de T. cruzi sendo a contagem dos parasitas feita pelo método de BRENER. Os resultados mostraram que a mediana pode impedir falsos resultados devido a valores muito altos ou muito baixos na parasitemia, porém não contém propriedades matemáticas que permita usá-la em inferencia estatística. As médias, com dados originais e transformados, foram comparadas entre si através dos respectivos coeficientes de variação (CV). Os resultados mostraram que a média logarítmica (Mlog) tem os menores valores de CV, sendo o melhor parâmetro para expressar a parasitemia quando os dados apresentam grande variabilidade. Estudamos também a influência do número de animais sôbre a variabilidade de dados usando a Mlog e o CVlog de grupos formados por 3, 5, 8 e 10 ratos; a análise estatística feita pelo teste de KRUSKAL-WALLIS não mostrou diferenças significantes entre os grupos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1993-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29075Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 5 (1993); 395-398 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29075/30932Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSogayar, RobertoKipnis, Tereza LiebermanCuri, Paulo Roberto2012-07-02T01:36:01Zoai:revistas.usp.br:article/29075Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:49.942816Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease Estudo crítico sobre a expressão da parasitemia na doença de Chagas experimental |
title |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
spellingShingle |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease Sogayar, Roberto Trypanosoma cruzi Parasitemia Critical evaluation |
title_short |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
title_full |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
title_fullStr |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
title_full_unstemmed |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
title_sort |
A critical evaluation of the expression of parasitemia in experimental Chagas' disease |
author |
Sogayar, Roberto |
author_facet |
Sogayar, Roberto Kipnis, Tereza Lieberman Curi, Paulo Roberto |
author_role |
author |
author2 |
Kipnis, Tereza Lieberman Curi, Paulo Roberto |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Sogayar, Roberto Kipnis, Tereza Lieberman Curi, Paulo Roberto |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Parasitemia Critical evaluation |
topic |
Trypanosoma cruzi Parasitemia Critical evaluation |
description |
This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used. |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29075 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29075/30932 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 5 (1993); 395-398 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951640510758912 |