A critical evaluation of the expression of parasitemia in experimental Chagas' disease

Detalhes bibliográficos
Autor(a) principal: Sogayar, Roberto
Data de Publicação: 1993
Outros Autores: Kipnis, Tereza Lieberman, Curi, Paulo Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29075
Resumo: This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used.
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spelling A critical evaluation of the expression of parasitemia in experimental Chagas' disease Estudo crítico sobre a expressão da parasitemia na doença de Chagas experimental Trypanosoma cruziParasitemiaCritical evaluation This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used. O objetivo deste trabalho foi o de estudar a melhor maneira de expressar a parasitemia de animais experimentalmente infectados com T. cruzi, visto que os animais podem apresentar grande variabilidade nos dados de parasitemia e que não é expressa nos resultados da maioria dos autores. Nós usamos um grupo de 50 ratos que foram infectados com 1x10(6) tripomastigotas da cepa Y de T. cruzi sendo a contagem dos parasitas feita pelo método de BRENER. Os resultados mostraram que a mediana pode impedir falsos resultados devido a valores muito altos ou muito baixos na parasitemia, porém não contém propriedades matemáticas que permita usá-la em inferencia estatística. As médias, com dados originais e transformados, foram comparadas entre si através dos respectivos coeficientes de variação (CV). Os resultados mostraram que a média logarítmica (Mlog) tem os menores valores de CV, sendo o melhor parâmetro para expressar a parasitemia quando os dados apresentam grande variabilidade. Estudamos também a influência do número de animais sôbre a variabilidade de dados usando a Mlog e o CVlog de grupos formados por 3, 5, 8 e 10 ratos; a análise estatística feita pelo teste de KRUSKAL-WALLIS não mostrou diferenças significantes entre os grupos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1993-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29075Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 5 (1993); 395-398 Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 5 (1993); 395-398 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29075/30932Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSogayar, RobertoKipnis, Tereza LiebermanCuri, Paulo Roberto2012-07-02T01:36:01Zoai:revistas.usp.br:article/29075Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:49.942816Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv A critical evaluation of the expression of parasitemia in experimental Chagas' disease
Estudo crítico sobre a expressão da parasitemia na doença de Chagas experimental
title A critical evaluation of the expression of parasitemia in experimental Chagas' disease
spellingShingle A critical evaluation of the expression of parasitemia in experimental Chagas' disease
Sogayar, Roberto
Trypanosoma cruzi
Parasitemia
Critical evaluation
title_short A critical evaluation of the expression of parasitemia in experimental Chagas' disease
title_full A critical evaluation of the expression of parasitemia in experimental Chagas' disease
title_fullStr A critical evaluation of the expression of parasitemia in experimental Chagas' disease
title_full_unstemmed A critical evaluation of the expression of parasitemia in experimental Chagas' disease
title_sort A critical evaluation of the expression of parasitemia in experimental Chagas' disease
author Sogayar, Roberto
author_facet Sogayar, Roberto
Kipnis, Tereza Lieberman
Curi, Paulo Roberto
author_role author
author2 Kipnis, Tereza Lieberman
Curi, Paulo Roberto
author2_role author
author
dc.contributor.author.fl_str_mv Sogayar, Roberto
Kipnis, Tereza Lieberman
Curi, Paulo Roberto
dc.subject.por.fl_str_mv Trypanosoma cruzi
Parasitemia
Critical evaluation
topic Trypanosoma cruzi
Parasitemia
Critical evaluation
description This paper aims to study the best way to express the parasitemia of Trypanosoma cruzi's experimentally infected animals. Individual scores may have a great variability, not emphasized by the majority of the authors. A group of 50 rats infected with 1x10(6) trypomastigotes of T. cruzi Y strain was used and the parasitemia was estimated by BRENER' s method. The results showed that the median can avoid false results due to very high or low parasitemias but it does not have the mathematic properties necessary for analysis of variance. The comparison of the means of the original and transformed data, with their respective coefficients of variability (CV), showed that the logarithmic mean (Mlog) have the minor value of CV. Therefore, the Mlog is the best way to express the parasitemia when the data show great variability. The number of the animal for group did not affect the variability of data when the Mlog and CV were used.
publishDate 1993
dc.date.none.fl_str_mv 1993-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29075
url https://www.revistas.usp.br/rimtsp/article/view/29075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29075/30932
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 No. 5 (1993); 395-398
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 35 Núm. 5 (1993); 395-398
Revista do Instituto de Medicina Tropical de São Paulo; v. 35 n. 5 (1993); 395-398
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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